Glutamic acid‐related hydrazine reagent for the derivatization of carbonyl compounds

Abstract

Chemical derivatization of carbonyl compounds is a prerequisite for their determination in environmental and biological samples by liquid chromatography-tandem mass spectrometry analysis. The main requirements regarding the good derivatization reagent are fast, selective, and quantitative reaction with carbonyl compounds and efficient fragmentation upon collision-induced dissociation. We have prepared glutamic acid-related hydrazine reagent and optimized derivatization procedure with benzaldehyde as a model carbonyl compound. The collision-induced dissociation spectrum of the obtained hydrazone gave rich fragmentation pattern with numerous abundant fragment ions. Structure-specific fragment ions were selected for the development of sensitive multiple reaction monitoring method.

Progress and Challenges in Quantifying Carbonyl-Metabolomic Phenomes with LC-MS/MS

“…Moreover, characteristic neutral losses and fragment ions produced by the CHH derivatives during the CID process can be used to clearly identify the carbonyl compounds. In addition, Brkljacic et al [85] designed a glutamic acid-related hydrazide reagent, which had higher sensitivity than DNPH owing to its higher ionization efficiency. Moreover, the fragmentations of glutamic acid-related hydrazones were structure specific and highly reproducible, which facilitated its use in sensitive MRM analysis.…”