Development of a Novel Scaffold Based on Basil Seed Gum/Chitosan Hydrogel Containing Quercetin-Loaded Zein Microshphere for Bone Tissue Engineering

Al-Musawi, Mastafa H.; Rashidi, Mohsen; Mohammadzadeh, Vahid; Albukhaty, Salim; Mahmoudi, Elham; Ghorbani, Marjan

doi:10.1007/s10924-023-02913-y

Cited by 13 publications

(1 citation statement)

References 78 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This type of scaffold must have mechanical properties that are as close to the bone as possible [46]. One of the most crucial considerations in manufacturing bone tissue scaffolds is su cient mechanical strength since it promotes tissue organization and enhances cell proliferation on the scaffold surface [47]. A suitable environment for cell adhesion and growth is created by strong scaffolds with a high compressive modulus, as they can withstand the tension and strain of native tissue [48].…”

Section: Mechanical Scaffold Characterizationmentioning

confidence: 99%

Development of 3D-Printed PCL/ Baghdadite Nanocomposite Scaffolds for Bone Tissue Engineering Applications

Emadi,

Baghani,

Khodaei

et al. 2024

J Polym Environ

View full text Add to dashboard Cite

A signi cant obstacle in bone tissue engineering is the creation of biodegradable bone replacements with the requisite mechanical and biological capabilities to treat more severe and intricately shaped injuries.Baghdadite has recently indicated that active biological ions such as silicon (Si 4+ ) and zirconium (Zr 4+ ) have been proven to increase bone growth considerably. In this study, we produced 3D-printed PCL-based scaffolds containing different amounts of Baghdadite using the robocasting solvent technique. Notably, PCL with 40 and 60 wt.% Baghdadite scaffolds (PB40 and PB60) promoted a more biomimetic environment for in vitro bone growth as their proper bioactivity and cell viability results were obtained without the addition of osteoinductive components. The printing process produced 3D scaffolds with a compressive strength of 7.94 MPa and elastic modulus of 29.95 MPa in PB40. According to the analytical prediction models in PB40, the elastic modulus was 24.7 and 26.89 MPa. Also, adding 60 wt.% Baghdadite increased the degradation rate to 5.1% in two months, more than six times that of PCL-based scaffolds. Cell proliferation assay demonstrated that the optical density of MG63 cells after 7 days of culture increased from 1.43 ± 0.03 to 1.82 ± 0.20 in PB40 as compared to pure PCL scaffold. Furthermore, bioactivity evaluation, ion release assessment, and morphological observation results further revealed that incorporating Baghdadite into a 3D-printed PCL-based scaffold could improve bone regeneration.Our ndings demonstrate that the PCL/Baghdadite composite scaffold may be e ciently manufactured using 3D-printing technology and is extremely promising for bone tissue engineering applications.

show abstract

Section: Mechanical Scaffold Characterizationmentioning

confidence: 99%

Development of 3D-Printed PCL/ Baghdadite Nanocomposite Scaffolds for Bone Tissue Engineering Applications

Emadi,

Baghani,

Khodaei

et al. 2024

J Polym Environ

View full text Add to dashboard Cite

show abstract

Microfluidic Droplet‐Based Tailorable Porous GelMA Microspheres for 3D Spatio Controllable Cell Coculture

Wang,

Du,

Hao

et al. 2024

Adv Materials Technologies

View full text Add to dashboard Cite

For the construction of accurate 3D tissue models in vitro, the 3D coculture model formed by spatio‐controlled distribution of multiple cells is essential. Despite the benefits of porous microspheres with low density, large surface area, and high permeability, exact spatio‐controlled distribution fails to appear in 3D coculture models by using porous microspheres as carriers. Herein, a facile method is proposed to construct a 3D coculture model with heterogeneous cell spatio‐tailored distribution using GelMA microspheres with customizable porosity structure and size based on droplet microfluidics in combination with placeholders. ATPS emulsions, containing two biocompatible immiscible aqueous phases of cell/Alg /dextran (Dex) mixture and polyethylene glycol (PEG) solution, are used as the templates to generate the placeholders. The micron‐sized porous GelMA microspheres are prepared by UV crosslinking of droplets prepared in microfluidic devices, followed by chelation of the placeholders. As a proof of concept, the 3D coculture hepatocellular carcinoma (HCC) model with HepG2 encapsulated outside the pores and HUVEC inside the pores is established. Noteworthy, the cells in the 3D coculture HCC model exhibit greater cell activity and proliferation, enhanced functionalities, and drug resistance than the control group. In conclusion, this straightforward method provides a novel approach for constructing 3D coculture models in vitro.

show abstract

Exploring the therapeutic potential of quercetin: A focus on its sirtuin‐mediated benefits

Ungurianu,

Zanfirescu,

Margină

2024

Phytotherapy Research

View full text Add to dashboard Cite

As the global population ages, preventing lifestyle‐ and aging‐related diseases is increasing, necessitating the search for safe and affordable therapeutic interventions. Among nutraceuticals, quercetin, a flavonoid ubiquitously present in various plants, has garnered considerable interest. This review aimed to collate and analyze existing literature on the therapeutic potentials of quercetin, especially its interactions with SIRTs and its clinical applicability based on its bioavailability and safety. This narrative review was based on a literature survey spanning from 2015 to 2023 using PUBMED. The keywords and MeSH terms used were: “quercetin” AND “bioavailability” OR “metabolism” OR “metabolites” as well as “quercetin” AND “SIRTuin” OR “SIRT*” AND “cellular effects” OR “pathway” OR “signaling” OR “neuroprotective” OR “cardioprotective” OR “nephroprotective” OR “antiatherosclerosis” OR “diabetes” OR “antidiabetic” OR “dyslipidemia” AND “mice” OR “rats”. Quercetin demonstrates multiple therapeutic activities, including neuroprotective, cardioprotective, and anti‐atherosclerotic effects. Its antioxidant, anti‐inflammatory, antiviral, and immunomodulatory properties are well‐established. At a molecular level, it majorly interacts with SIRTs, particularly SIRT1 and SIRT6, and modulates numerous signaling pathways, contributing to its therapeutic effects. These pathways play roles in reducing oxidative stress, inflammation, autophagy regulation, mitochondrial biogenesis, glucose utilization, fatty acid oxidation, and genome stability. However, clinical trials on quercetin's effectiveness in humans are scarce. Quercetin exhibits a wide range of SIRT‐mediated therapeutic effects. Despite the compelling preclinical data, more standardized clinical trials are needed to fully understand its therapeutic potential. Future research should focus on addressing its bioavailability and safety concerns.

show abstract

Development of a Novel Scaffold Based on Basil Seed Gum/Chitosan Hydrogel Containing Quercetin-Loaded Zein Microshphere for Bone Tissue Engineering

Cited by 13 publications

References 78 publications

Development of 3D-Printed PCL/ Baghdadite Nanocomposite Scaffolds for Bone Tissue Engineering Applications

Development of 3D-Printed PCL/ Baghdadite Nanocomposite Scaffolds for Bone Tissue Engineering Applications

Microfluidic Droplet‐Based Tailorable Porous GelMA Microspheres for 3D Spatio Controllable Cell Coculture

Exploring the therapeutic potential of quercetin: A focus on its sirtuin‐mediated benefits

Contact Info

Product

Resources

About