Development of a novel pulsatile bioreactor for tissue culture

Morsi, Yos S.; Yang, William; Owida, Amal; Wong, Cynthia S.

doi:10.1007/s10047-006-0369-5

Cited by 32 publications

(29 citation statements)

References 18 publications

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“…The prevailing view is that the classic in vitro culture environment does not adequately replicate the physical forces and chemical concentration gradients present in vivo. This has led to the development of several alternative culture systems (bioreactors) in which the dynamic cell culture devices allow the simulation of the physiological conditions of an organism [1][2][3]. The goal of an effective bioreactor is to influence and control biological reactions and functions.…”

Section: Introductionmentioning

confidence: 99%

Hydrostatic pressure and shear stress affect endothelin‐1 and nitric oxide release by endothelial cells in bioreactors

Vozzi

Bianchi

Ahluwalia

et al. 2013

Biotechnology Journal

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Abundant experimental evidence demonstrates that endothelial cells are sensitive to flow; however, the effect of fluid pressure or pressure gradients that are used to drive viscous flow is not well understood. There are two principal physical forces exerted on the blood vessel wall by the passage of intra-luminal blood: pressure and shear. To analyze the effects of pressure and shear independently, these two stresses were applied to cultured cells in two different types of bioreactors: a pressure-controlled bioreactor and a laminar flow bioreactor, in which controlled levels of pressure or shear stress, respectively, can be generated. Using these bioreactor systems, endothelin-1 (ET-1) and nitric oxide (NO) release from human umbilical vein endothelial cells were measured under various shear stress and pressure conditions. Compared to the controls, a decrease of ET-1 production by the cells cultured in both bioreactors was observed, whereas NO synthesis was up-regulated in cells under shear stress, but was not modulated by hydrostatic pressure. These results show that the two hemodynamic forces acting on blood vessels affect endothelial cell function in different ways, and that both should be considered when planning in vitro experiments in the presence of flow. Understanding the individual and synergic effects of the two forces could provide important insights into physiological and pathological processes involved in vascular remodeling and adaptation.

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Section: Introductionmentioning

confidence: 99%

Hydrostatic pressure and shear stress affect endothelin‐1 and nitric oxide release by endothelial cells in bioreactors

Vozzi

Bianchi

Ahluwalia

et al. 2013

Biotechnology Journal

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“…8,15,17,20,21,23,24,33 In the majority of pulse reactors varying levels of pressures and fluid waveforms are controlled manually. 8,17 The pulsatile bioreactor developed by Hildebrand et al uses a computer-controlled closed-loop feedback system and generates highly controlled pulsatile waveforms.…”

Section: Discussionmentioning

confidence: 99%

“…These pulse reactors use external flow circuits, which usually occupy a lot of space in the incubator and are complicated to handle for long-term experiments. Pulsatile flow is realized by external pistons, 8 tube compression by pressurized air, 21 a balloon chamber, 24 or by diaphragms fibrillated by fluids 23,33 or pressurized air. 17,23 Usually these systems are designed to test one heart valve at a time.…”

Section: Discussionmentioning

confidence: 99%

Simplified Pulse Reactor for Real-Time Long-Term In Vitro Testing of Biological Heart Valves

Schleicher

Sammler

Schmauder³

et al. 2010

Ann Biomed Eng

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Long-term function of biological heart valve prostheses (BHV) is limited by structural deterioration leading to failure with associated arterial hypertension. The objective of this work was development of an easy to handle real-time pulse reactor for evaluation of biological and tissue engineered heart valves under different pressures and long-term conditions. The pulse reactor was made of medical grade materials for placement in a 37 degrees C incubator. Heart valves were mounted in a housing disc moving horizontally in culture medium within a cylindrical culture reservoir. The microprocessor-controlled system was driven by pressure resulting in a cardiac-like cycle enabling competent opening and closing of the leaflets with adjustable pulse rates and pressures between 0.25 to 2 Hz and up to 180/80 mmHg, respectively. A custom-made imaging system with an integrated high-speed camera and image processing software allow calculation of effective orifice areas during cardiac cycle. This simple pulse reactor design allows reproducible generation of patient-like pressure conditions and data collection during long-term experiments.

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“…1,15,20,22 Mechanical stimulation plays a key role in enhancing cell adhesion and proliferation as well as in stimulating the biosynthesis of the extracellular matrix (ECM) components and their structural architecture. Mechanical stimulation in dynamic bioreactors for VC growth usually relied on shear stress stimulation, 13 strain stimulation, 19,24 or a combination of the two; 8,10,14,21,23 cyclic flexural stimulation was also experimented. 4,5 The so-called diastolic pulsed stimulation (DPS) 18,19 is a particular strain-based mechanical stimulation that is performed by applying a pulsatile trans-valvular pressurization while the VC is kept in its closed configuration.…”

Section: Introductionmentioning

confidence: 99%

“…The data were obtained by setting the phantom VC's compliance (C phan ) in the range of 0.048-0.175 mL mmHg21 . The bars represent the standard deviation.…”

mentioning

confidence: 99%

A Bioreactor with Compliance Monitoring for Heart Valve Grafts

et al. 2009

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The drawbacks of state-of-the-art heart valve prostheses lead researchers to explore the prospect of using tissue-engineered constructs as possible valve substitutes. It is widely accepted that the mechanical properties of the construct are improved with mechanical stimulation during in vitro growth. We designed a new dynamic bioreactor with the perspective of using decellularized valve homografts as scaffolds in order to produce tissue-engineered valve substitutes. The design guidelines were (a) compatibility with the procedures for the treatment of homografts; (b) delivery of finely controlled pulsatile pressure loads, which induce strain stimuli that may drive cells toward repopulation of and integration with the natural scaffold; and (c) monitoring the construct's biomechanical status through a comprehensive index, i.e., its compliance. The handling needs during the set-up of the homograft and the use of the bioreactor were minimized. The bioreactor and its automated control system underwent tests with a compliant phantom valve. The estimated compliances are in good agreement with the measured ones. Tests were also carried out with porcine aortic samples in order to assess the hydrodynamic and biomechanical reliability. In the future, monitoring the construct's compliance might represent a key factor in controlling the recellularization of the valve homografts, which provides awareness of the construct's biomechanical status by real-time, non-destructive, and non-invasive means.

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Development of a novel pulsatile bioreactor for tissue culture

Cited by 32 publications

References 18 publications

Hydrostatic pressure and shear stress affect endothelin‐1 and nitric oxide release by endothelial cells in bioreactors

Hydrostatic pressure and shear stress affect endothelin‐1 and nitric oxide release by endothelial cells in bioreactors

Simplified Pulse Reactor for Real-Time Long-Term In Vitro Testing of Biological Heart Valves

A Bioreactor with Compliance Monitoring for Heart Valve Grafts

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