Development of a novel high-performance thin-layer chromatographic-densitometric method for the detection of tallow adulteration in cow ghee

De, Subrata; Nariya, Pankaj; Jirankalgikar, Nikhil

doi:10.1556/jpc.26.2013.6.5

Cited by 4 publications

(1 citation statement)

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“…Though vegetable oil is readily detected in ghee at very low levels, [3][4][5] detection of body fat adulteration in ghee is still a challenge [6] as the analysis of physico-chemical characteristics alone is not very much helpful in detecting the presence of animal body fat in ghee. Similarly, some modern analytical techniques like derivative spectroscopy [7] and the TLC-based method [8] could not detect adulteration of ghee with animal body fat at a lower level. In addition to this, the said two techniques have only been used only in detecting the adulteration of cow ghee, whereas in India both cow milk and buffalo milk are commonly used for the preparation of ghee.…”

Section: Introductionmentioning

confidence: 99%

A species specific simplex polymerase chain reaction-based approach for detection of goat tallow in heat clarified milk fat (ghee)

Hazra

Sharma

et al. 2017

International Journal of Food Properties

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The detection of milk fat adulteration with foreign fats/oils continues to be a challenge for the dairy industry as well as food testing laboratories. Recently, various chromatographic methods have been developed to detect a low level of vegetable oil in ghee but detection of animal body fat in ghee is still a challenge. In India, goat body fat (goat tallow) adulteration in ghee is a very serious problem. In the present investigation, a rapid species specific polymerase chain reaction (PCR)-based methodology has been standardised to detect goat tallow in heat clarified milk fat (ghee). This standardised PCR-based protocol did not show any false positive results in the genuine ghee (heat clarified butter fat) samples. Adulteration of ghee (both cow and buffalo) with goat tallow at the 10% level could be detected using the standardised PCR-based protocol. The protocol standardised is rapid and convenient to use. ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 99%