A review of thin layer chromatography methods for determination of authenticity of foods and dietary supplements

Sherma, Joseph; Rabel, Fred

doi:10.1080/10826076.2018.1505637

Cited by 26 publications

(15 citation statements)

References 72 publications

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“…KLT merupakan teknik pemisahan yang digunakan dalam analisis bahan alam, pangan, penentuan alkaloid; antosianidin; antrakuinon; antibiotik; amina, amida, asam amino; karbohidrat; flavonoid; lemak; fenol; pigmen dan pewarna; racun; vitamin; dan jenis aneka senyawa lainnya (Sherma & Rabel, 2018). Kelebihan metode KLT dibandingkan kromatografi lainnya yaitu metodenya sederhana dan memiliki ketelitian yang baik.…”

Section: Pendahuluanunclassified

“…Silika gel yang digunakan merupakan silika gel 60 F254 yang memiliki indikator fluoresensi sehingga dapat bersinar ketika diberikan UV panjang gelombang 254nm dan akan menghasilkan pita pemisahan warna gelap, sedangkan ketika disinari UV 366nm, adsorben ini tidak akan mengalami fluoresensi sehingga latar belakang yang dihasilkan adalah berwarna gelap. Sebaliknya senyawa pada pita mengandung gugus kromofor dan terikat pada ausokrom, mengalami interaksi dengan UV 366nm sehingga menghasilkan pita pemisahan yang bersinar (Sherma & Rabel, 2018…”

Section: 03) a B C D E F G A B C D E F G A B C D E F G (A) (B) (C)unclassified

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PROFIL KROMATOGRAFI LAPIS TIPIS EKSTRAK KUNYIT (Curcuma longa Linn) SEGAR DAN SIMPLISIA DENGAN VARIASI METODE EKSTRAKSI

Kautsari¹,

Purwakusumah

Nurcholis

2021

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Turmeric is a rhizome plant used as traditional Indonesian medicine. Generally, the extraction process influences the efficacy of turmeric in curing a disease. This study compares the thin layer chromatography profile (TLC) of turmeric extract based on variations in the extraction method. The extraction process was carried out using 70% ethanol solvent in fresh rhizomes and turmeric simplicia. The study used maceration, sonication, and microwave assistance techniques. The thin layer chromatography profiles were analyzed using curcuminoid standards in visible and UV light visualization (254 and 366 nm). The results showed higher yields in the fresh turmeric extracts through maceration extraction, sonication, and microwave-assisted techniques, respectively. The thin layer chromatography pattern using eluent chloroform dichloromethane (32.5: 67.5 v / v) showed the extract was separated into 3 -4 compounds, with different curcuminoid thicknesses in each treatment. The thin layer chromatography profile with the highest quality of curcuminoid content was found successively in sonication simplicia, maceration, and microwave simplicia extracts, as well as sonication fresh turmeric extract, maceration, and microwaves. The high yield of turmeric extract does not indicate an increase in the quality of the curcuminoid levels produced.

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Section: Pendahuluanunclassified

Section: 03) a B C D E F G A B C D E F G A B C D E F G (A) (B) (C)unclassified

PROFIL KROMATOGRAFI LAPIS TIPIS EKSTRAK KUNYIT (Curcuma longa Linn) SEGAR DAN SIMPLISIA DENGAN VARIASI METODE EKSTRAKSI

Kautsari¹,

Purwakusumah

Nurcholis

2021

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“…Thin layer chromatography is an easy-to-operate, rapid and cost-effective chromatographic technique which has been widely used in food analysis for several decades [76,77]. Its main advantages compared to column chromatography comprise to its simplicity, minimum demands for sample preparation and solvent consumption and the possibility to simultaneously analyze multiple samples side-by-side.…”

Section: Analytical Techniquesmentioning

confidence: 99%

“…Its main advantages compared to column chromatography comprise to its simplicity, minimum demands for sample preparation and solvent consumption and the possibility to simultaneously analyze multiple samples side-by-side. TLC, coupled with UV, densitometric and mass spectrometric detection, has been used to determine several classes of fruit juice constituents such as carotenoids, vitamins, sugars and polyphenols [76,77]. However, so far, there are only few applications of TLC to fruit juice authenticity assessment and quality control (Table 4).…”

Section: Analytical Techniquesmentioning

confidence: 99%

Quality and Authenticity Control of Fruit Juices-A Review

Dasenaki

Thomaidis

2019

Molecules

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Food fraud, being the act of intentional adulteration of food for financial advantage, has vexed the consumers and the food industry throughout history. According to the European Committee on the Environment, Public Health and Food Safety, fruit juices are included in the top 10 food products that are most at risk of food fraud. Therefore, reliable, efficient, sensitive and cost-effective analytical methodologies need to be developed continuously to guarantee fruit juice quality and safety. This review covers the latest advances in the past ten years concerning the targeted and non-targeted methodologies that have been developed to assure fruit juice authenticity and to preclude adulteration. Emphasis is placed on the use of hyphenated techniques and on the constantly-growing role of MS-based metabolomics in fruit juice quality control area.

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“…Thin layer chromatography (TLC) represents a simple and easily scalable method for the detection of diverse compounds in plant extracts, herbal products and foods [ 17 , 18 ]. When paired with chromatogram densitometry or image analysis for compound quantification, TLC provides a cost-effective alternative to other detection methods (e.g., HPLC, NIS, RS) for high-throughput sample screening [ 18 ]. Marker compound screening using TLC-densitometry/image analysis requires the establishment of a protocol that ensures reliable compound separation and visualization.…”

Section: Introductionmentioning

confidence: 99%

Visualization of Aspalathin in Rooibos (Aspalathus linearis) Plant and Herbal Tea Extracts Using Thin-Layer Chromatography

et al. 2019

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Aspalathin, the main polyphenol of rooibos (Aspalathus linearis), is associated with diverse health promoting properties of the tea. During fermentation, aspalathin is oxidized and concentrations are significantly reduced. Standardized methods for quality control of rooibos products do not investigate aspalathin, since current techniques of aspalathin detection require expensive equipment and expertise. Here, we describe a simple and fast thin-layer chromatography (TLC) method that can reproducibly visualize aspalathin in rooibos herbal tea and plant extracts at a limit of detection (LOD) equal to 178.7 ng and a limit of quantification (LOQ) equal to 541.6 ng. Aspalathin is a rare compound, so far only found in A. linearis and its (rare) sister species A. pendula. Therefore, aspalathin could serve as a marker compound for authentication and quality control of rooibos products, and the described TLC method represents a cost-effective approach for high-throughput screening of plant and herbal tea extracts.

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A review of thin layer chromatography methods for determination of authenticity of foods and dietary supplements

Cited by 26 publications

References 72 publications

PROFIL KROMATOGRAFI LAPIS TIPIS EKSTRAK KUNYIT (Curcuma longa Linn) SEGAR DAN SIMPLISIA DENGAN VARIASI METODE EKSTRAKSI

PROFIL KROMATOGRAFI LAPIS TIPIS EKSTRAK KUNYIT (Curcuma longa Linn) SEGAR DAN SIMPLISIA DENGAN VARIASI METODE EKSTRAKSI

Quality and Authenticity Control of Fruit Juices-A Review

Visualization of Aspalathin in Rooibos (Aspalathus linearis) Plant and Herbal Tea Extracts Using Thin-Layer Chromatography

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