Development of a novel comprehensive analytical method for volatile compounds using supercritical fluid chromatography/mass spectrometry with a highly cross-linked styrene divinylbenzene polymer-based column

A review of retention mechanism studies for packed column supercritical fluid chromatography

“…On these stationary phases, the analyte retention increases when either hydrocarbon molecular volume or solute polarity increases, and the selectivity shows significant changes 105–108 . Fujito et al reported that the retentions of volatile compounds having multiple double bonds or aromatic rings were strong on the highly cross‐linked styrene‐divinylbenzene polymer‐based column with almost the same retention order obtained on the phenyl column 90 . Vera et al reported the differences in polycyclic aromatic hydrocarbon (PAHs) selectivity on two aromatic columns, that are Synergi Polar‐RP column (ether‐linked phenyl phase with proprietary hydrophilic end‐capping) and Cosmosil 5PBB column (with pentabromobenzyl group bonded silica) 108 .…”

“…Another way to avoid non‐specific interactions is using polymer‐based stationary phases. For instance, Fujito et al reported the use of novel highly cross‐linked styrene‐divinylbenzene polymer‐based stationary phase, characterized by hydrophobicity, zero silanol groups, π‐π interaction, high specific surface area, and high durability under SFC conditions for retaining 23 compounds including flavors, aromas, and fragrances 90 . The stationary phase nature also affects the analyte retention 13,53,87,91,92 .…”

“…Whereas, despite the stationary phases and the mobile phase compositions, several types of interactions between the analytes and the stationary phases all contributed to the analyte retention in pSFC. Fujito et al confirmed that the retention behavior on the NH 2 stationary phase was strongly affected by the number of hydroxyl groups (OH − ) 90 . Addition of one OH − on an aromatic acid increases the separation factor on both BEH silica and BEH 2‐EP stationary phases; however, the separation factors on BEH 2‐EP is much better 95 .…”

Recent trends in the chromatographic analysis of volatile flavor and fragrance compounds: Annual review 2020

“…Using this column it was possible to retain compounds like certain esters and non‐polar terpenes that could not be retained on regular silica‐based columns (C 18 , phenyl, and NH 2 ). The authors demonstrated that volatile compounds with a wide range of chemical properties can successfully be analyzed by SFC using the new SDVB column 109 …”

Comparison of Supercritical Fluid Chromatography Hyphenated to an Ultraviolet Detector and Gas Chromatography Hyphenated to a Flame Ionization Detector for Qualitative and Quantitative Analysis of Citrus Essential Oils

“…In 2020, Fujito et al have demonstrated the ability to separate volatile compounds such as α-pinene, limonene, and citral using a styrene-divinyl benzene (SDVB) polymer-based column in SFC mode [22]. The resolution observed on the chromatograms between pinene (k' = 2.3), citral (k' = 3.5), and limonene (k' = 3.2) was quite low.…”