Development of a new wound dressing with antimicrobial delivery capability

Abstract

A bilaminar wound dressing composed of an outer membrane and an inner three-dimensional matrix of a fabric or a sponge may be considered to constitute an ideal structure that promotes wound healing: the outer membrane prevents body fluid loss, controls water evaporation, and protects the wound surface from bacterial invasion, and the inner matrix encourages adherence by tissue growth into the matrix. Using this concept, we developed a biosynthetic wound dressing with a drug delivery capability. This medicated wound dressing is composed of a spongy sheet of a chitosane derivative and collagen mixture that is laminated to an antimicrobial-impregnated polyurethane membrane. In this study, a gentamycin sulfate-impregnated wound dressing was prepared and evaluated. The antimicrobial efficacy of this wound dressing was examined on an agar plate seeded with Pseudomonas aeruginosa. Also, the cytotoxicity of an antimicrobial released from this wound dressing was examined in an in vitro system with cultured skin substitutes. Both in vitro tests have shown that this wound dressing is capable of suppressing bacterial growth and minimizing cellular damage. In addition, in the treatment of wounds inflicted on rats and rabbits, this wound dressing was shown to be efficacious in covering full-thickness and split-thickness skin defects. Finally, the efficacy of this wound dressing was evaluated in a nonrandomized open-label study of 31 clinical cases. In 31 cases treated with this wound dressing, good or excellent wound healing was achieved.

Preparation of <i>N</i>-Succinyl-chitosan and Their Physical-Chemical Properties as a Novel Excipient

“…Suc-Chi displays good water soluble property at various pHs. 6) Suc-Chi was initially developed as wound dressing materials, 7) it is currently also applied as cosmetic materials (Moistˆne liquid ). 8) New wound dressings composed of Suc-Chi and gelatin were also developed.…”

Organoselenium Coating on Cellulose Inhibits the Formation of Biofilms byPseudomonas aeruginosaandStaphylococcus aureus

“…Antibiotics, silver, or chitosan, attached to or embedded in gauze, have been shown to be efficacious in preventing wound infection (21,24,26,36). However, the resistance of P. aeruginosa and S. aureus to available antibiotics severely limits the choices for antibiotic treatment (13,40).…”

In Vivo Evaluation of Kumazasa Extract and Chitosan Films Containing the Extract against Deep Skin Ulcer Model in Rats

“…1,2) Various dosage forms have been developed to treat skin ulcers such as pressure ulcers. [3][4][5][6] We also prepared a patch with a tamarind gum-sucrose hydrogel containing silver sulfadiazine, and demonstrated that the formulation displayed a better effect than Geben ® cream. 7,8) Generally, a pressure ulcer goes through the stages of an period of infection and necrosis, an agglutination period, and a proliferation period of granulation and epidermis in that order until cure.…”

“…To each stage, many formulations, including ointments, creams, films and dressings, have been developed. [9][10][11][12][13][14] Ointments containing minocycline, which exhibited controlled release, were reported recently because minocycline is highly effective against pressure ulcer bacteria.15) Many formulations applied to pressure ulcers possess an antibacterial function 6,7,11,12,14,15) or potential to enhance the proliferation of granulation or epidermis. 4,[16][17][18] Namely, substances with such abilities and high safety can be candidates as agents for the treatment of pressure ulcers.…”

“…15) Many formulations applied to pressure ulcers possess an antibacterial function 6,7,11,12,14,15) or potential to enhance the proliferation of granulation or epidermis. 4,[16][17][18] Namely, substances with such abilities and high safety can be candidates as agents for the treatment of pressure ulcers.…”