Development of a new set of molecular markers for examining Glu-A1 variants in common wheat and ancestral species

Dong, Zhenying; Yang, Yan; Zhang, Kunpu; Li, Yiwen; Wang, Junjun; Wang, Zhaojun; Liu, Xin; Qin, Huanju; Wang, Daowen

doi:10.1371/journal.pone.0180766

Cited by 5 publications

(2 citation statements)

References 50 publications

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“…Several end-use seed quality markers are diagnostic as they have been established based on the sequences of the relevant genes [51,68]. A new set of DNA markers helped to establish 11 haplotypes at the Glu-A1 locus (H1 to H11) in three wheat species [69]. The major haplotypes in tetraploid wheat (T. turgidum) are H1, H8, and H9, containing both 1Ax and 1Ay subunits.…”

Section: Tetraploid and Hexaploid Triticum Speciesmentioning

confidence: 99%

Allelic Variation of High-Molecular-Weight Glutenin Genes in Triticum Species and Triticale (× Triticosecale Wittmack)

Spetsov,

Daskalova

2024

OBM Genet

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High-molecular-weight glutenin subunits (HMW-GS) encoded by alleles at the Glu-A1, Glu-B1, and Glu-D1 loci confer unique bread-making properties of common wheat (Triticum aestivum L.). The identification of HMW-GS is the prerequisite for pyramiding high-quality glutenin genes. The present review is designed to list all published HMW-GS alleles in Triticum species and triticale (A- and B genomes), focusing on methods for their identification. T. monococcum is characterized by 37 alleles at the Glu-1 locus versus four alleles in T. thaoudar and 39 in T. urartu. In total, 80 alleles at Glu-A1 of diploid Triticum species and about 42 alleles found in polyploid wheat landraces and varieties (4x and 6x), including triticale, were listed. Allelic variation at the Glu-B1 locus is divided into 3 groups: a – z, aa – az, and ba – ct, comprising 121 alleles, of which 26 subunits have unspecified alleles. At least 51 allelic variants at locus Glu-D1 of Triticum species were indicated, along with carriers of the species level. In addition, subunit-specific genetic loci have been tagged, facilitating molecular marker development of high-gluten wheat cultivars through marker-assisted breeding.

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Section: Tetraploid and Hexaploid Triticum Speciesmentioning

confidence: 99%

Allelic Variation of High-Molecular-Weight Glutenin Genes in Triticum Species and Triticale (× Triticosecale Wittmack)

Spetsov,

Daskalova

2024

OBM Genet

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“…In these cases, although the linkage drag of deleterious alleles linked to desirable alleles in the exotic parent, as a result of the wild nature of this source material, might be a handicap [29], the notable variation in both technological quality aspects and adaptive traits could compensate for any negative impact of the linkage drag. In this respect, the development of DNA markers that facilitate the selection of alleles of interest in a breeding program by marker-assisted selection will be key [30,31].…”

Section: Introductionmentioning

confidence: 99%

Potential Use of Wild Einkorn Wheat for Wheat Grain Quality Improvement: Evaluation and Characterization of Glu-1, Wx and Ha Loci

et al. 2021

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Wild einkorn (Triticum monococcum L. ssp. aegilopoides (Link) Thell.) is a diploid wheat species from the Near East that has been classified as an ancestor of the first cultivated wheat (einkorn; T. monococcum L. ssp. monococcum). Its genome (Am), although it is not the donor of the A genome in polyploid wheat, shows high similarity to the Au genome. An important characteristic for wheat improvement is grain quality, which is associated with three components of the wheat grain: endosperm storage proteins (gluten properties), starch synthases (starch characteristics) and puroindolines (grain hardness). In the current study, these grain quality traits were studied in one collection of wild einkorn with the objective of evaluating its variability with respect to these three traits. The combined use of protein and DNA analyses allows detecting numerous variants for each one of the following genes: six for Ax, seven for Ay, eight for Wx, four for Gsp-1, two for Pina and three for Pinb. The high variability presence in this species suggests its potential as a source of novel alleles that could be used in modern wheat breeding.

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Genomic and functional genomics analyses of gluten proteins and prospect for simultaneous improvement of end-use and health-related traits in wheat

Wang

Cao

et al. 2020

Theor Appl Genet

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Key message Recent genomic and functional genomics analyses have substantially improved the understanding on gluten proteins, which are important determinants of wheat grain quality traits. The new insights obtained and the availability of precise, versatile and high-throughput genome editing technologies will accelerate simultaneous improvement of wheat end-use and health-related traits. Abstract Being a major staple food crop in the world, wheat provides an indispensable source of dietary energy and nutrients to the human population. As worldwide population grows and living standards rise in both developed and developing countries, the demand for wheat with high quality attributes increases globally. However, efficient breeding of high-quality wheat depends on critically the knowledge on gluten proteins, which mainly include several families of prolamin proteins specifically accumulated in the endospermic tissues of grains. Although gluten proteins have been studied for many decades, efficient manipulation of these proteins for simultaneous enhancement of end-use and health-related traits has been difficult because of high complexities in their expression, function and genetic variation. However, recent genomic and functional genomics analyses have substantially improved the understanding on gluten proteins. Therefore, the main objective of this review is to summarize the genomic and functional genomics information obtained in the last 10 years on gluten protein chromosome loci and genes and the cis- and trans-factors regulating their expression in the grains, as well as the efforts in elucidating the involvement of gluten proteins in several wheat sensitivities affecting genetically susceptible human individuals. The new insights gathered, plus the availability of precise, versatile and high-throughput genome editing technologies, promise to speed up the concurrent improvement of wheat end-use and health-related traits and the development of high-quality cultivars for different consumption needs.

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Development of a new set of molecular markers for examining Glu-A1 variants in common wheat and ancestral species

Cited by 5 publications

References 50 publications

Allelic Variation of High-Molecular-Weight Glutenin Genes in <i>Triticum</i> Species and Triticale (× <i>Triticosecale</i> Wittmack)

Allelic Variation of High-Molecular-Weight Glutenin Genes in <i>Triticum</i> Species and Triticale (× <i>Triticosecale</i> Wittmack)

Potential Use of Wild Einkorn Wheat for Wheat Grain Quality Improvement: Evaluation and Characterization of Glu-1, Wx and Ha Loci

Genomic and functional genomics analyses of gluten proteins and prospect for simultaneous improvement of end-use and health-related traits in wheat

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