Development of a new rhodamine-based FRET platform and its application as a Cu2+ probe

Abstract

We have constructed a new rhodamine-based FRET platform, which was then used to develop a ratiometric fluorescent Cu(2+) probe. The novel Cu(2+) probe exhibits several favorable features including a large variation in the emission ratio, well-resolved emission peaks, high sensitivity, and high selectivity. Importantly, it is suitable for fluorescence imaging in living cells.

Highly sensitive and fast responsive ratiometric fluorescent probe for Cu2+ based on a naphthalimide-rhodamine dyad and its application in living cell imaging

“…Part 1 and part 2 was synthesized by reported methods (the detailed synthetic routes were shown in Supporting information) [29,36].…”

“…From this point of view, fluorescence resonance energy transfer (FRET) based sensors were the most valuable [21][22][23][24]. To the best of our knowledge, only a few FRET probes for detecting Cu 2+ based on rhodamine have been reported [25][26][27][28][29][30].…”

A phenothiazine–rhodamine ratiometric fluorescent probe for Hg2+ based on FRET and ICT

“…The location, variations in concentration, and environment of the probe may change the emission collection efficiency and excitation intensity, which may further influence the accuracy and reliability of measurements. 5,6 In order to overcome these influences, ratiometric fluorescent probes have been developed. 7 Ratiometric fluorescent probes measure changes in the ratio of emission intensities at two different wavelengths.…”

A colorimetric chemosensor for the sequential detection of copper(II) and cysteine

“…S2) [47]. This value is within those (10 3~1 0 12 ) previously reported for Cu 2+ -binding chemosensors [48][49][50][51][52][53]. Based on the result of UV-vis titration, the detection limit for 1-Cu 2+ complex was determined to be 2.4 µM on basis of 3σ/K (Fig.…”