Development of a new multi-mode NIR laser system for photodynamic therapy

Kamanlı, Ali Furkan; Yıldız, Mustafa Zahid; Arslan, Halil; Çetinel, Gökçen; Lim, Necla Kenar; Lim, Hyun Kyoung

doi:10.1016/j.optlastec.2020.106229

Cited by 8 publications

(2 citation statements)

References 34 publications

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“…The system has three modes to choose from: CW, pulse, and burst pulse. The modes were developed considering the application modalities indicated in the literature [ 23 , 24 ]. The user can select the experiment duration and mode-specific parameters for each mode separately.…”

Section: Methodsmentioning

confidence: 99%

Development of a novel laboratory photodynamic therapy device: automated multi-mode LED system for optimum well-plate irradiation

Yildiz,

Kamanli,

Eskiler

et al. 2024

Lasers Med Sci

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Photodynamic therapy (PDT) is a targeted treatment method that utilizes a photosensitizer (PS) to induce cytotoxicity in malignant and non-malignant tumors. Optimization of PDT requires investigation of the selectivity of PS for the target tissues, irradiating light source, irradiation wavelengths, fluence rate, fluence, illumination mode, and overall treatment plan. In this study, we developed the Multi-mode Automatized Well-plate PDT LED Laboratory Irradiation System (MAWPLIS), an innovative device that automates time-consuming well plate light dosage/PS dose measurement experiment. The careful control of LED current and temperature stabilization in the LED module allowed the system to achieve high optical output stability. The MAWPLIS was designed by integrating a 3-axis moving system and motion controller, a quick-switching LED controller unit equipped with interchangeable LED modules capable of employing multiple wavelengths, and a TEC system. The proposed system achieved high optical output stability (1 mW) within the range of 0-500 mW, high wavelength stability (5 nm) at 635 nm, and high temperature stability (0.2 °C) across all radiation modes. The system’s validation involved in vitro analysis using 5-ALA across varying concentrations, incubation periods, light exposures, and wavelengths in HT-29 colon cancer and WI-38 human lung fibroblast cell lines. Specifically, a combination of 405 nm and 635 nm wavelengths was selected to demonstrate enhanced strategies for colon cancer cell eradication and system validation. The MAWPLIS system represents a significant advancement in photodynamic therapy (PDT) research, offering automation and standardization of time-intensive experiments, high stability and precision, and improved PDT efficacy through dual-wavelength integration.

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Section: Methodsmentioning

confidence: 99%

Development of a novel laboratory photodynamic therapy device: automated multi-mode LED system for optimum well-plate irradiation

Yildiz,

Kamanli,

Eskiler

et al. 2024

Lasers Med Sci

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“…The device, detailed information about which can be found in Ref. [18], was designed for use together with the photo-sensitizers that have relatively higher absorption around 635 nm. For example, 5-aminolevulinic acid (5-ALA) is medically used effectively together with 635 nm light sources [19,20] .…”

Section: Methodsmentioning

confidence: 99%

Untitled

2021

中国光学快报

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In this study, the effects of purification, dehydration, and coagulation processes on the absorption and reduced scattering coefficients of chicken liver tissues have been investigated by using a single integrating sphere system. The purification process performed on the tissue samples to remove blood residue has been found to cause a slight change in the optical parameters. Although the dehydration process brings about an increase in the absorption coefficient due to the water loss, no direct relationship has been observed between the reduced scattering coefficient and the dehydration level of the tissue. In addition, it has been observed that there was a relatively small increase in the absorption coefficient and a significant increase in the reduced scattering coefficient after the coagulation process. Therefore, it can be said that the optical penetration depth decreased significantly after dehydration and coagulation processes unlike blood purification. Moreover, fluence rate distributions inside the fresh, blood purified, dehydrated, and coagulated tissue models have been investigated by using the Monte Carlo modeling of photon transport in multilayered tissues simulation code.

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