Investigation of LED-based photodynamic therapy efficiency on breast cancer cells

Kamanlı, Ali Furkan; Yıldız, Mustafa Zahid; Özyol, Ebru; Özkan, Asuman Deveci; Kucukkara, Elif Sozen; Eskiler, Gamze Güney

doi:10.1007/s10103-020-03061-8

Cited by 9 publications

(5 citation statements)

References 42 publications

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“…PDT is usually defined as a powerful inducer of tumor-cell apoptosis [ 33 ]. The rate of apoptosis increased after ALAD-PDT, a sign that its administration causes an arrest in the proliferation of both cell lines but at different times.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, 5-ALA-based PDT can be a potential therapeutic strategy for the treatment of different tumors. In preclinical studies the anti-tumor efficacy of 5-ALA-mediated PDT has been observed in different cancer types such as skin cancer [ 30 ], esophageal cancer [ 31 ], colon cancer [ 28 ], human glioma [ 32 ], breast cancer [ 33 ], bladder cancer [ 34 ], and hepatocellular carcinoma [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

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Effect of 5-Aminolevulinic Acid (5-ALA) in “ALADENT” Gel Formulation and Photodynamic Therapy (PDT) against Human Oral and Pancreatic Cancers

D’Antonio,

Marchetti,

Pignatelli

et al. 2024

Biomedicines

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Oral squamous-cell and pancreatic carcinomas are aggressive cancers with a poor outcome. Photodynamic therapy (PDT) consists of the use of photosensitizer-induced cell and tissue damage that is activated by exposure to visible light. PDT selectively acts on cancer cells, which have an accumulation of photosensitizer superior to that of the normal surrounding tissues. 5-aminolevulinic acid (5-ALA) induces the production of protoporphyrin IX (PpIX), an endogenous photosensitizer activated in PDT. This study aimed to test the effect of a new gel containing 5% v/v 5-ALA (ALAD-PDT) on human oral CAL-27 and pancreatic CAPAN-2 cancer cell lines. The cell lines were incubated in low concentrations of ALAD-PDT (0.05%, 0.10%, 0.20%, 0.40%, 0.75%, 1.0%) for 4 h or 8 h, and then irradiated for 7 min with 630 nm RED light. The cytotoxic effects of ALAD-PDT were measured using the MTS assay. Apoptosis, cell cycle, and ROS assays were performed using flow cytometry. PpIX accumulation was measured using a spectrofluorometer after 10 min and 24 and 48 h of treatment. The viability was extremely reduced at all concentrations, at 4 h for CAPAN-2 and at 8 h for CAL-27. ALAD-PDT induced marked apoptosis rates in both oral and pancreatic cancer cells. Elevated ROS production and appreciable levels of PpIX were detected in both cell lines. The use of ALA-PDT as a topical or intralesional therapy would permit the use of very low doses to achieve effective results and minimize side effects. ALAD-PDT has the potential to play a significant role in complex oral and pancreatic anticancer therapies.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of 5-Aminolevulinic Acid (5-ALA) in “ALADENT” Gel Formulation and Photodynamic Therapy (PDT) against Human Oral and Pancreatic Cancers

D’Antonio,

Marchetti,

Pignatelli

et al. 2024

Biomedicines

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“…Recent research has shifted its focus towards LED (Light Emitting Diode) light sources for PTT due to their affordability and reduced side effects. LED-based PTT offers a more accessible option for research laboratories and clinical facilities 91 . In the past, the treatment of tumors primarily relied on the use of tightly focused, synchronized, and highly uniform beams of light with the potential for high power densities.…”

Section: Effect Of Led In Pttmentioning

confidence: 99%

Recent Trends in Bio-nanomaterials and Non-invasive Combinatorial Approaches of Photothermal Therapy against Cancer

Beniwal,

Verma,

Putta

et al. 2024

Nanotheranostics

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In 2020, approximately 10 million deaths worldwide were attributed to cancer, making it the primary cause of death globally. Photothermal therapy (PTT) is one of the novel ways to treat and abolish cancer. PTT significantly impacts cancer theranostics compared to other therapies like surgery, chemotherapy, and radiotherapy due to its remarkable binding capability to tumor sites and lower invasiveness into normal healthy tissues. PTT relies on photothermal agents (PTAs), which generate heat by absorbing the near-infrared (NIR) light and destroying cancer cells. Several PTT agents remain longer in the reticuloendothelial system (RES) and induce toxicity, restricting their use in the biomedical field. To overcome this problem, the usage of biodegradable nano-photothermal agents is required. This review has discussed the PTT mechanism of action and different types of novel bio-nanomaterials used for PTT. We also focussed on the combinatorial effects of PTT with other cancer therapies and their effect on human health. The role of LED lights and mild hypothermia in PTT has been discussed briefly in this review.

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“…Another study focused on the effect of 5-ALA-PDT mediated LED laser lights for the source of PDT application. LED irradiation was used on two breast cancer cell lines: MCF-7 and MDA-MB-231 [61]. The effectiveness of this therapy was checked by WST-1, annexin V, and acridine orange stain.…”

Section: Breast Cancermentioning

confidence: 99%

Reactive Oxygen Species Produced by 5-Aminolevulinic Acid Photodynamic Therapy in the Treatment of Cancer

Pignatelli,

Umme,

D’Antonio

et al. 2023

IJMS

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Cancer is the leading cause of death worldwide and several anticancer therapies take advantage of the ability of reactive oxygen species to kill cancer cells. Added to this is the ancient hypothesis that light alone can be used to kill cancer cells. 5-aminolevulinic acid-photodynamic therapy (5-ALA-PDT) is a therapeutic option for a variety of cutaneous and internal malignancies. PDT uses a photosensitizer that, activated by light in the presence of molecule oxygen, forms ROS, which are responsible for the apoptotic activity of the malignant tissues. 5-ALA is usually used as an endogenous pro-photosensitizer because it is converted to Protoporphyrin IX (PpIX), which enters into the process of heme synthesis and contextually becomes a photosensitizer, radiating a red fluorescent light. In cancer cells, the lack of the ferrochelatase enzyme leads to an accumulation of PpIX and consequently to an increased production of ROS. PDT has the benefit of being administered before or after chemotherapy, radiation, or surgery, without impairing the efficacy of these treatment techniques. Furthermore, sensitivity to PDT is unaffected by the negative effects of chemotherapy or radiation. This review focuses on the studies done so far on 5-ALA-PDT and its efficacy in the treatment of various cancer pathologies.

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Investigation of LED-based photodynamic therapy efficiency on breast cancer cells

Cited by 9 publications

References 42 publications

Effect of 5-Aminolevulinic Acid (5-ALA) in “ALADENT” Gel Formulation and Photodynamic Therapy (PDT) against Human Oral and Pancreatic Cancers

Effect of 5-Aminolevulinic Acid (5-ALA) in “ALADENT” Gel Formulation and Photodynamic Therapy (PDT) against Human Oral and Pancreatic Cancers

Recent Trends in Bio-nanomaterials and Non-invasive Combinatorial Approaches of Photothermal Therapy against Cancer

Reactive Oxygen Species Produced by 5-Aminolevulinic Acid Photodynamic Therapy in the Treatment of Cancer

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