Development of a new invasion and metastasis model of human oral squamous cell carcinomas

Kawashiri, Shin‐ya; Kumagai, Shinya; Kojima, Kazuhiko; Harada, Hiroki; Yamamoto, Etsuhide

doi:10.1016/0964-1955(95)00027-f

Cited by 55 publications

(46 citation statements)

References 18 publications

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“…127 Kawashiri et al compared intralingual, floor of mouth and ectopic subcutaneous implantation of two human HNSCC cell lines. 145,146 More aggressive behavior with regard to regional and distant metastasis was seen in the orthotopic models when compared with the ectopic subcutaneous implantation model. Likewise, Myers et al utilizing different oral SCC cell lines showed a more aggressive growth pattern for tumor cells injected intralingually when compared with similar cells injected subcutaneously.…”

Section: Orthotopic Modifications Of the Original Nude Mouse Modelmentioning

confidence: 99%

Animal models for the study of squamous cell carcinoma of the upper aerodigestive tract: A historical perspective with review of their utility and limitations. Part A. Chemically‐induced de novo cancer, syngeneic animal models of HNSCC, animal models of transplanted xenogeneic human tumors

Smith

Thomas

2005

Intl Journal of Cancer

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Understanding the complex histological, genetic and molecular changes that lead to malignant transformation of squamous epithelia of the head and neck will likely guide the development of methods for improved diagnosis, monitoring and treatment of head and neck squamous cell carcinoma (HNSCC). The development and use of animal models that closely mimic the histopathology and molecular pathogenesis of HNSCC in humans would greatly expand the research possibilities and provide a means of testing potential therapeutic agents. However, many available animal models of HNSCC fall short of this objective. In order for investigators to select the appropriate model to answer scientific questions, it is important to understand the benefits and limitations of available animal models for the study of HNSCC. The purpose of this work is to give an overview of the most pertinent animal models of HNSCC, and to discuss future directions of research in this field. ' 2005 Wiley-Liss, Inc.Head and neck squamous cell carcinoma (HNSCC) involves the upper aerodigestive tract and can destroy the structure and function of organs involved in voice, speech, taste, smell and hearing, as well as vital structures necessary for survival. Approximately half of all patients with HNSCC have advanced stage disease at the time of diagnosis, with an expected 5-year survival rate between 10 and 40%.1 Despite treatments that may consist of mutilating surgery, radiotherapy and/or chemotherapy, overall long-term survival remains low due to uncontrollable persistent or recurrent disease. The survival rate of patients with locoregional and distant recurrences has highlighted the need for new approaches for diagnosis and treatment. To this end, a better understanding of the genetic, molecular and immunoregulatory processes leading to neoplastic transformation and progression of HNSCC is needed, so that novel and more effective diagnostic and therapeutic approaches can be designed.Although in vitro study systems are useful for studying the interactions between different cell populations in culture and are convenient and simple to use, they do not reproduce the complexity of HNSCC. For this reason, the development of new therapies will require clinically relevant animal models of HNSCC. Adequate animal models are necessary to test the translational potential of preventive and therapeutic agents, as well as to understand the biological mechanisms of epithelial carcinogenesis and progression. The ideal experimental animal tumor model for HNSCC must reproduce the characteristic features of the disease, including the complicated patterns of tumor-host interactions such as immune response, angiogenesis, invasion and metastasis. In the ideal model, clinical symptoms and laboratory abnormalities should be similar to the human disease and therapeutic agents should be as effective in both systems. The ideal model must also be easy to use, accessible to genetic and immunologic manipulations, reproducible and progress rapidly to allow timely investigations.Animal mod...

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Section: Orthotopic Modifications Of the Original Nude Mouse Modelmentioning

confidence: 99%

Animal models for the study of squamous cell carcinoma of the upper aerodigestive tract: A historical perspective with review of their utility and limitations. Part A. Chemically‐induced de novo cancer, syngeneic animal models of HNSCC, animal models of transplanted xenogeneic human tumors

Smith

Thomas

2005

Intl Journal of Cancer

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“…HLA-A2 ϩ SCCHN cell lines, PCI-13 and PCI-1, were established from tumor biopsies as described (15). OSC-19 is an SCCHN cell line donated by E. Yamamoto, Kanazawa Univ., Kanazawa, Japan (16). PCI-13 cells were transfected with the hIL-2 gene, using the retroviral DFG-IL-2-Neo vector (PCI-13͞IL-2), as described (14).…”

Section: Methodsmentioning

confidence: 99%

Human tumor-derived genomic DNA transduced into a recipient cell induces tumor-specific immune responsesexvivo

Whiteside

Gambotto

Albers

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

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“…In contrast, when transplanted into the oral cavities of nude mice, the tumours showed invasive growth. 9 Another biological characteristic is that regional neck lymph node metastases are detected in more than 80% of cases. The histological characteristics are diffuse invasion into the surrounding tissue with cord-like microtumour nests, well-differentiated squamous cell carcinomas, and fibroblastic reaction and mononuclear cell infiltrates around the tumour nest.…”

Section: Micementioning

confidence: 99%

USPIO-enhanced MRI of highly invasive and highly metastasizing transplanted human squamous cell carcinoma: an experimental study

Tetsumura

Nakamura²,

Yoshino³

et al. 2012

Dentomaxillofacial Radiology

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Objective: The aim of this study was to investigate the signal intensity characteristics of highly invasive and highly metastasizing transplanted human squamous cell carcinoma using ultra-small super-paramagnetic iron oxide (USPIO)-enhanced MRI and to correlate them with USPIO distribution to tumour components revealed by histological examination. Methods: 13 nude mice with transplanted human squamous cell carcinoma in the oral cavity were imaged before and 24 hours after intravenous administration of USPIO. The difference in signal intensity between pre-contrast and post-contrast MR images was visually evaluated. For quantitative analysis, signal intensity within a region of interest was measured. Histological findings were correlated with MR findings. The approximate USPIO concentration was evaluated using USPIO phantoms. Results: Seven tumours had an area showing signal intensity increase on post-contrast T 1 weighted images. Histopathologically, six of those tumours contained a small amount of iron particles in the stroma. The USPIO concentration was presumed low. Two tumours had an area showing signal intensity decrease on post-contrast T 1 and T 2 weighted images. The areas had a large amount of iron particles in the stroma and the USPIO concentration was presumed high. There was a minimal amount of iron particles in tumour parenchymal cells. Conclusions: The amount of USPIO accumulation into tumour stroma was considered to affect MR signal intensity. A small amount increases T 1 weighted signal intensity, whereas a large amount decreases T 1 and T 2 weighted intensity. The USPIO accumulation into the tumour parenchyma was not thought to affect MR signal intensity.

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Development of a new invasion and metastasis model of human oral squamous cell carcinomas

Cited by 55 publications

References 18 publications

Animal models for the study of squamous cell carcinoma of the upper aerodigestive tract: A historical perspective with review of their utility and limitations. Part A. Chemically‐induced de novo cancer, syngeneic animal models of HNSCC, animal models of transplanted xenogeneic human tumors

Animal models for the study of squamous cell carcinoma of the upper aerodigestive tract: A historical perspective with review of their utility and limitations. Part A. Chemically‐induced de novo cancer, syngeneic animal models of HNSCC, animal models of transplanted xenogeneic human tumors

Human tumor-derived genomic DNA transduced into a recipient cell induces tumor-specific immune responsesexvivo

USPIO-enhanced MRI of highly invasive and highly metastasizing transplanted human squamous cell carcinoma: an experimental study

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