Development of a new biosensor based on functionalized SBA-15 modified screen-printed graphite electrode as a nano-reactor for Gquadruplex recognition

Bagheryan, Zahra; Raoof, Jahan Bakhsh; Ojani, Reza; Rezaei, Parizad

doi:10.1016/j.talanta.2013.09.052

Cited by 17 publications

(7 citation statements)

References 35 publications

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“…In the last years, researches have devoted their efforts in the study of SBA-15, which these molecular sieves because of their technological applications, such as drug delivery [5], biosensors [6], thermal energy storage [7], luminescence [8,9], and biomedical imaging [10]. Particularly, especial attention has been devoted to SBA-15 because of its 2D hexagonal pore structure (average diameters between 2 and 10 nm), high surface area (typically >800 m 2 /g) [11,12], and high capacity for adsorption of toxic heavy metals {Cu(II), Zn(II), Cd(II), Ni(II), and Pb(II)} [13,14].…”

Section: Introductionmentioning

confidence: 99%

Anatase TiO2 nanocrystals anchored at inside of SBA-15 mesopores and their optical behavior

Araújo

Silva

Sczancoski

et al. 2016

Applied Surface Science

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In this paper, a new synthesis method was proposed to obtain anatase titanium oxide (TiO 2) nanocrystals anchored into SBA-15 molecular sieve, as a matrix assigned by the in-situ anchoring (ISA) method. Pure SBA-15 and modified with TiO 2 nanocrystals at different Si/Ti molar ratios (R = 75, 50, and 25) were structurally characterized by X-ray diffraction (XRD), Micro-Raman and Fourier Transform infrared (FTIR) spectroscopies. Specific surface area, pore volume and average pore diameter were estimated using both Brunauer-Emmett-Teller (BET) and Barrett-Joyner-Halenda (BJH) methods, respectively. Morphological aspects of these samples were observed by means of field emission scanning electron microscopy (FE-SEM) and transmission electron microscopy (TEM). Optical properties were investigated by ultravioletvisible (UV-vis) diffuse reflectance spectroscopy. XRD patterns, Micro-Raman and FT-IR spectra indicate the TiO 2 nanocrystals crystallized in a tetragonal structure anchored into the SBA-15 mesopores. BET and BJH methods prove a large amount of TiO 2 nanocrystals were anchored inside of SBA-15 mesopores due to increase in surface area and average pore size of SBA-15 matrix. FE-SEM and TEM images showed the pure SBA-15 has an elongated hexagon-shaped microstructure, and an average size of 7.34 nm for 2D hexagonal mesopores. Moreover, ISA method was able to avoid blocking of mesopores, in addition promotes a significant increasing the impregnation rate of anatase TiO 2 nanocrystals in SBA-15 matrix. A growth mechanism was proposed in order to explain the stages involved in the formation of TiO 2-SBA mesoporous. UV-vis spectra revealed a dependence of the optical band gap energy (E gap) with the decreasing of Si/Ti molar ratios.

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Section: Introductionmentioning

confidence: 99%

Anatase TiO2 nanocrystals anchored at inside of SBA-15 mesopores and their optical behavior

Araújo

Silva

Sczancoski

et al. 2016

Applied Surface Science

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“…[13][14][15] Tam belongs to a class of drugs known as selective estrogen receptor modulators, and was one of the earliest medications employed for targeted therapies in breast cancer. 16 In continuation of our previous studies on the electrochemical features of G 4 DNA-ligand interaction, 17,18 in the present paper we describe a new nanobiosensor, constructed using G-quadruplex, for the electrochemical investigation of a number of ligands able to stabilize this structure. A graphite screen-printed electrode (SPE), which is low in cost and massproducible, 19,20 was modified with functionalized SiO 2 nanoparticles (SiO 2 @NPPNSH), and AuNPs were then introduced to the electrode surface, and the organic SH groups on SiO 2 @NPPNSH were able to bind to the AuNPs on the electrode surface.…”

Section: Introductionmentioning

confidence: 63%

A human telomeric G-quadruplex-based electronic nanoswitch for the detection of anticancer drugs

Bagheryan

Raoof

Ojani

et al. 2015

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An electronic nanoswitch is described based on the conformational change of the DNA sequence in the presence of stabilizing ligands. The new electrochemical biosensor was prepared by modifying a screen-printed graphite electrode (SPE) with functionalized SiO2 nanoparticles [(SiO2-N-propylpiperazine-N-(2-mercaptopropane-1-one) (SiO2@NPPNSH)] and Au nanoparticles (AuNPs). These nanoparticles are able to immobilize thiolated G-quadruplex DNA structures (SH-G4DNA). The SH groups on the SiO2@NPPNSH nanoparticles provide a good platform for stabilizing AuNPs on the surface of the electrode. This is due to the fact that AuNPs are able to bind to the organic SH groups on the SiO2@NPPNSH. The SH-G4DNA binds to the modified electrode by a AuNPs-S bond. The structure of SiO2@NPPNSH was characterized by scanning electron microscopy (SEM), thermo-gravimetric analysis (TGA) and infrared (IR) spectroscopy. The morphology of the modified electrode was characterized by SEM. The interaction between G4DNA and the anticancer drug, Tamoxifen (Tam), was studied in Tris-HCl buffer and [Fe(CN)6](3-) using cyclic (CV) and square wave voltammetry (SWV). The G-quadruplex formation and the interaction mechanism were identified by circular dichroism (CD) measurements. The CV current was seen to decrease with increasing concentration of Tam due to interaction between G4DNA and Tam. This biosensor is a simple and useful tool for selecting G-quadruplex-binding ligands.

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“…Our method does not need complicated facilities such as PCR amplication, electrophoresis and hybridization follow-up processing, it greatly reduced the risk of contamination [24,30]. Along with the progress of micromachining technology and nanotechnology, the molecular motor biosensor has been largely applied in the various detection fields [31][32][33][34][35]. Further miniaturization and portability of our method would allow expanding this technology to wider applications.…”

Section: Validation Of the Assay Methods With Actual Samplesmentioning

confidence: 99%

A rapidly new-typed detection of norovirus based on F0F1-ATPase molecular motor biosensor

Zhao¹,

Zhang²,

Xu³

et al. 2016

Biotechnol Bioproc E

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In order to adapt port rapid detection of food borne norovirus, presently we developed a new typed detection method based on F 0 F 1 -ATPase molecular motor biosensor. A specific probe was encompassed the conservative region of norovirus and F 0 F 1 -ATPase within chromatophore was constructed as a molecular motor biosensor through the "ε-subunit antibody-streptomycinbiotin-probe" system. Norovirus was captured based on probe-RNA specific binding. Our results demonstrated that the Limit of Quantification (LOQ) is 0.005 ng/mL for NV RNA and also demonstrated that this method possesses specificity and none cross-reaction for food borne virus. What's more, the experiment used this method could be accomplished in 1 h. We detected 10 samples by using this method and the results were consistent with RT-PCR results. Overall, based on F 0 F 1 -ATPase molecular motors biosensor system we firstly established a new typed detection method for norovirus detection and demonstrated that this method is sensitive and specific and can be used in the rapid detection for food borne virus.

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Development of a new biosensor based on functionalized SBA-15 modified screen-printed graphite electrode as a nano-reactor for Gquadruplex recognition

Cited by 17 publications

References 35 publications

Anatase TiO2 nanocrystals anchored at inside of SBA-15 mesopores and their optical behavior

Anatase TiO2 nanocrystals anchored at inside of SBA-15 mesopores and their optical behavior

A human telomeric G-quadruplex-based electronic nanoswitch for the detection of anticancer drugs

A rapidly new-typed detection of norovirus based on F0F1-ATPase molecular motor biosensor

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