Development of a nanoparticle-based system for the delivery of retinoic acid into macrophages

Almouazen, Eyad; Bourgeois, Sandrine; Boussaid, A.; Valot, Pascale; Malleval, Céline; Fessi, Hatem; Nataf, Serge; Briançon, Stéphanie

doi:10.1016/j.ijpharm.2012.03.025

Cited by 38 publications

(28 citation statements)

References 47 publications

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“…atRA quantification in nanoemulsion was monitored using a RP-HPLC method (Agilent 1200 series) as previously described by Almouazen et al [37]. Briefly, C18 column with 2.6 µm particle size (Phenomenex, Kinetex) was used as a stationary phase.…”

Section: High Performance Liquid Chromatographymentioning

confidence: 99%

Comparison of Three Processes for Parenteral Nanoemulsion Production: Ultrasounds, Microfluidizer, and Premix Membrane Emulsification

Alliod

Almouazen

Nemer

et al. 2019

Journal of Pharmaceutical Sciences

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Nanoemulsions are of great interest for pharmaceutical applications, including parenteral dosage forms. However, their production is still limited and requires more efficient and adaptive technologies. The more common systems are high-shear homogenization like microfludizers (MF) at industrial scale and ultrasounds at research scale, both based on high energy limiting their application for sensitive drugs. Recently, a process based on premix membrane emulsification (PME) was developed to produce nanoemulsions. These three processes have been compared for the production of a model parenteral nanoemulsion containing all-transretinoic acid, a thermolabile molecule which is used in the treatment of acute promyelocytic leukemia in a parenteral form. Droplet size and active integrity were studied because of their major interest for efficacy and safety assessment. Regarding droplet size, PME produced monodispersed droplets of 335 nm compared to the other processes which produced nanoemulsions of around 150 nm but with the presence of micron size droplets detected by laser diffraction and optical microscopy. No real difference between the three processes was observed on active degradation during emulsifcation. However, regarding stability, especially at 40 o C nanoemulsions obtained with the microfluidizer showed a greater molecule degradation and unstable nanoemulsion with a 4 times droplet size increase under stress conditions.

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Section: High Performance Liquid Chromatographymentioning

confidence: 99%

Comparison of Three Processes for Parenteral Nanoemulsion Production: Ultrasounds, Microfluidizer, and Premix Membrane Emulsification

Alliod

Almouazen

Nemer

et al. 2019

Journal of Pharmaceutical Sciences

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“…In the delivery of curcumin into glioma cells, early time points evaluated showed higher uptake for the non‐encapsulated drug, whereas for late time points (48 h or more), uptake was increased by nanoencapsulation . Nanocapsules are usually taken up together with the encapsulated substance into the cytosol, in the endosomal compartment or in the micronucleus . PLA‐nanocapsules were described to remain inside cells for a period of at least 7 days without complete degradation .…”

Section: Advantages Of Polymeric Nanocapsules For Drug Deliverymentioning

confidence: 99%

“…Nanocapsules are usually taken up together with the encapsulated substance into the cytosol, in the endosomal compartment or in the micronucleus . PLA‐nanocapsules were described to remain inside cells for a period of at least 7 days without complete degradation . After internalization, the drug is released by simple diffusion, by polymer enzymatic degradation, or by redox‐responsive degradation of the polymer .…”

Section: Advantages Of Polymeric Nanocapsules For Drug Deliverymentioning

confidence: 99%

Improving drug biological effects by encapsulation into polymeric nanocapsules

Frank

Contri

Beck

et al. 2015

WIREs Nanomed Nanobiotechnol

130

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This review is based on selected reports from 2004 to 2014 and provides a comprehensive and updated overview of the state of the art related to the drug delivery advantages of polymeric nanocapsules, which are a specific type of polymeric nanoparticles used for improvement of biological effects. Special attention is given to the application of nanocapsules to increase the chemical and photostability of drugs, to modulate the interaction with cells and tissues, to reduce adverse effects of drugs, and to increase the drug efficiency and/or bioavailability. Moreover, this review covers in vitro and in vivo studies, highlighting interesting examples of drugs from several therapeutic classes for which efficacy is improved by encapsulation in different types of nanocapsules, especially in lipid-core nanocapsules. We also briefly present the first results obtained so far attesting to the safety of using polymeric nanocapsules for drug delivery.

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“…[1][2][3] In cancer therapeutics, polymeric nanocarriers offer the added advantage of specific organ and subcellular targeting either into the cytoplasm, nucleus, or other specialized organelles. This makes polymeric nanocarriers ideal candidates for the subdelivery of both bio-and chemotherapeutic agents as they enhance clinical efficacy while minimizing the incidence of side effects.…”

Section: Introductionmentioning

confidence: 99%

Polycaprolactone/maltodextrin nanocarrier for intracellular drug delivery: formulation, uptake mechanism, internalization kinetics, and subcellular localization

Korang-Yeboah¹,

Gorantla²,

Paulos³

et al. 2015

IJN

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Prostate cancer (PCa) disease progression is associated with significant changes in intracellular and extracellular proteins, intracellular signaling mechanism, and cancer cell phenotype. These changes may have direct impact on the cellular interactions with nanocarriers; hence, there is the need for a much-detailed understanding, as nanocarrier cellular internalization and intracellular sorting mechanism correlate directly with bioavailability and clinical efficacy. In this study, we report the differences in the rate and mechanism of cellular internalization of a biocompatible polycaprolactone (PCL)/maltodextrin (MD) nanocarrier system for intracellular drug delivery in LNCaP, PC3, and DU145 PCa cell lines. PCL/MD nanocarriers were designed and characterized. PCL/MD nanocarriers significantly increased the intracellular concentration of coumarin-6 and fluorescein isothiocyanate-labeled bovine serum albumin, a model hydrophobic and large molecule, respectively. Fluorescence microscopy and flow cytometry analysis revealed rapid internalization of the nanocarrier. The extent of nanocarrier cellular internalization correlated directly with cell line aggressiveness. PCL/MD internalization was highest in PC3 followed by DU145 and LNCaP, respectively. Uptake in all PCa cell lines was metabolically dependent. Extraction of endogenous cholesterol by methyl-β-cyclodextrin reduced uptake by 75%±4.53% in PC3, 64%±6.01% in LNCaP, and 50%±4.50% in DU145, indicating the involvement of endogenous cholesterol in cellular internalization. Internalization of the nanocarrier in LNCaP was mediated mainly by macropinocytosis and clathrin-independent pathways, while internalization in PC3 and DU145 involved clathrin-mediated endocytosis, clathrin-independent pathways, and macropinocytosis. Fluorescence microscopy showed a very diffused and non-compartmentalized subcellular localization of the PCL/MD nanocarriers with possible intranuclear localization and minor colocalization in the lysosomes with time.

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Development of a nanoparticle-based system for the delivery of retinoic acid into macrophages

Cited by 38 publications

References 47 publications

Comparison of Three Processes for Parenteral Nanoemulsion Production: Ultrasounds, Microfluidizer, and Premix Membrane Emulsification

Comparison of Three Processes for Parenteral Nanoemulsion Production: Ultrasounds, Microfluidizer, and Premix Membrane Emulsification

Improving drug biological effects by encapsulation into polymeric nanocapsules

Polycaprolactone/maltodextrin nanocarrier for intracellular drug delivery: formulation, uptake mechanism, internalization kinetics, and subcellular localization

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Development of a nanoparticle-based system for the delivery of retinoic acid into macrophages

Cited by 38 publications

References 47 publications

Comparison of Three Processes for Parenteral Nanoemulsion Production: Ultrasounds, Microfluidizer, and Premix Membrane Emulsification

Comparison of Three Processes for Parenteral Nanoemulsion Production: Ultrasounds, Microfluidizer, and Premix Membrane Emulsification

Improving drug biological effects by encapsulation into polymeric nanocapsules

Polycaprolactone/maltodextrin nanocarrier for intracellular drug delivery: formulation, uptake mechanism, internalization kinetics, and&nbsp;subcellular localization

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Polycaprolactone/maltodextrin nanocarrier for intracellular drug delivery: formulation, uptake mechanism, internalization kinetics, and subcellular localization