Development of a multi-tiered approach to the in vitro prescreening of clay-based enterosorbents

Lemke, Shawna L.; Ottinger, Sean; Mayura, K.; Ake, Charles; Pimpukdee, Komkrich; Wang, N; Phillips, Timothy D.

doi:10.1016/s0377-8401(01)00272-3

Cited by 58 publications

(44 citation statements)

References 20 publications

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“…Many studies have been performed to evaluate different commercial products intended to be used as mycotoxin binding agents and various aspects have been discussed [21,26,[43][44][45][46][47][48][49][50]. Masoero et al [49] concluded that physical processing of feed, such as pelleting, had a significant effect on the ability of added sequestering agent to reduce levels of AFM1 in milk when cattle was fed with AFB1 contaminated feed [49].…”

Section: Detoxification Of Animal Feed From Mycotoxinsmentioning

confidence: 99%

“…However, all potential mycotoxin binding agents must be tested in vivo to confirm their efficacy and safety and lack of interactions with nutrients. An approach for an effective pre-screening mycotoxin/sorbent combinations before testing on animals has been suggested [50]. The in vitro testing system developed so far, involved several investigations:…”

Section: Detoxification Of Animal Feed From Mycotoxinsmentioning

confidence: 99%

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Evaluation of the effect of mycotoxin binders in animal feed on the analytical performance of standardised methods for the determination of mycotoxins in feed

Kolosova

Stroka

2012

Food Additives & Contaminants: Part A

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Section: Detoxification Of Animal Feed From Mycotoxinsmentioning

confidence: 99%

Section: Detoxification Of Animal Feed From Mycotoxinsmentioning

confidence: 99%

Evaluation of the effect of mycotoxin binders in animal feed on the analytical performance of standardised methods for the determination of mycotoxins in feed

Kolosova

Stroka

2012

Food Additives & Contaminants: Part A

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“…The in vitro study is often first step in the evaluation of the capacity of clays to adsorb aflatoxins because it is easy to perform in the laboratory without using animals. In most in vitro studies, the adsorption reaction used to be done in an aqueous environment at neutral pH (16,21,23,28,29) and only a few studies have been carried out in a dynamic gastrointestinal environment model (30,31,32). Unfortunately, some methods tend to oversimplify the complexity of the gastrointestinal tract by performing the adsorption process at only a single pH while others are too complex in attempting to mimic the digestive process, which is difficult to manage.…”

Section: Introductionmentioning

confidence: 99%

In vitro evaluation of the capacity of zeolite and bentonite to adsorb aflatoxin B1 in simulated gastrointestinal fluids

Thieu

Pettersson

2008

Mycotox Res

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Anin vitro study using single concentration and isotherm adsorption was carried out to evaluate the capacity of Vietnamese produced zeolite and bentonite to adsorb aflatoxin B1 (AFB1) in simulated gastrointestinal fluids (SGFs), and a commercial sorbent hydrated sodium calcium aluminosilicate (HSCAS) was used as reference. In this study, AFB1 solution was mixed with sorbents (0.3, 0.4 and 0.5% w/v) in SGFs at pH 3 and pH 7 and shaken for 8 h, centrifuged and the supernatant measured by Vicam fluorometer. Adsorption of AFB1 onto zeolite and bentonite varied according to the pH of SGFs and was lower than HSCAS. Linearity between the increased amount of AFB1 adsorbed on sorbents and the decrease of sorbent concentration was observed for bentonite and HSCAS, except for zeolite in SGFs at pH 7. The observed maximum amounts of AFB1 adsorbed on bentonite and HSCAS were 1.54 and 1.56 mg/g, respectively. The adsorption capacities of bentonite and HSCAS for AFB1 were 12.7 and 13.1 mg/g, respectively, from fitting the data to the Freundlich isotherm equation. Improvement in processing and purification for bentonite is needed to enhance the surface area, which would probably result in better adsorptive capacity for this sorbent.

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“…In vitro (Lemke et al, 2001) and in vivo (Harvey et al, 1989a(Harvey et al, ,b, 1991aBonna et al, 1991;Schell et al, 1993) studies have shown that one of the more promising approaches to this problem is to add sorbents to animal diets. Such sorbents can sequester mycotoxins, reducing their absorption from the gastrointestinal tract.…”

Section: Introductionmentioning

confidence: 99%

Aflatoxin B1 Binding to Sorbents in Bovine Ruminal Fluid

et al. 2005

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A recent approach to the problem of contamination of agricultural products by aflatoxin B(1) (AFB(1)) is to add non-nutritional adsorbents to animal diets in order to sequester ingested aflatoxins. We conducted in vitro experiments to develop a rapid and cheap model using ruminal fluid to assess the ability of sorbent materials to bind AFB(1). Seven sorbents (hydrated sodium calcium aluminosilicate; clinoptilolite; zeolite; two types of bentonite; sepiolite; and PHIL 75), commonly added to bovine diets were incubated in water and ruminal fluid in the presence of AFB(1). Hydrated sodium calcium aluminosilicate, sepiolite and one of the bentonites bound 100% of the AFB(1) in the presence of both ruminal fluid and water; clinoptilolite bound about 80% of AFB(1) in both liquids; whereas the affinities for the mycotoxin of zeolite (50%) and the other sample of bentonite (60%) in water seem to be increased by about 40% in ruminal fluid incubations. PHIL 75 had the poorest binding ability: about 30% in water and 45% in ruminal fluid. In view of the differences in toxin binding in water and ruminal fluid, it is preferable to use the ruminal fluid model for the in vitro pre-screening of sorbent materials potentially useful as adjuvants to ruminant feeds.

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Development of a multi-tiered approach to the in vitro prescreening of clay-based enterosorbents

Cited by 58 publications

References 20 publications

Evaluation of the effect of mycotoxin binders in animal feed on the analytical performance of standardised methods for the determination of mycotoxins in feed

Evaluation of the effect of mycotoxin binders in animal feed on the analytical performance of standardised methods for the determination of mycotoxins in feed

In vitro evaluation of the capacity of zeolite and bentonite to adsorb aflatoxin B1 in simulated gastrointestinal fluids

Aflatoxin B1 Binding to Sorbents in Bovine Ruminal Fluid

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