Development of a multi-pathogen enrichment broth for simultaneous growth of five common foodborne pathogens

Abstract

The objective of the present study was to formulate a multi-pathogen enrichment broth which could support the simultaneous growth of five common foodborne pathogens (Salmonella enterica, Staphylococcus aureus, Shigella flexneri, Listeria monocytogenes and Escherichia coli O157:H7). The formulated broth SSSLE was composed of potassium tellurite, bile salt, lithium chloride, and sodium chloride as growth-inhibitors; glucose, esculin, mannitol and sodium pyruvate as growth-promoters. Compared with the respective specific selective enrichment broths, the individual growth pattern of each target pathogen in SSSLE was equal, or even better, except in the case of S. flexneri. In mixed-culture experiments, the gram-negative bacteria showed higher growth capabilities than the gram-positive bacteria after 8-h enrichment; however, the cell numbers after 24-h enrichment indicated that SSSLE could support the concurrent growth of five target pathogens irrespective of whether pathogens were inoculated initially at equal or unequal levels. For natural food samples under the high background flora, the final cell numbers enriched in SSSLE for five targets were enough to be detected by multiplex PCR. In conclusion, SSSLE was capable of supporting the growth of five target pathogens concurrently. The new broth formulated in this study has the potential of saving time, efforts and costs in multi-pathogen enrichment procedures.

Synergistic antimicrobial effects of activated lactoferrin and rosemary extract in vitro and potential application in meat storage

“…Large numbers of foodborne diseases originate from the presence of pathogenic bacteria in food that consequently cause serious health concerns and economic cost (Chen et al 2015). Although the application of several food preservation methods, proliferation of bacteria and production of several toxins result in food poisonings (Gonelimali et al 2018;Quinto et al 2019).…”

Development of an Enteric Bacterial Enrichment Broth and Its Performance for Isolation of Clinically Significant Bacterial Pathogens from Stool

“…Xiao et al (2010) developed an enrichment broth to support simultaneous growth of Salmonella, Vibrio parahaemolyticus, and Vibrio cholerae [26]. The most promising multiplex enrichment broth effort was the development of the SSSLE broth supporting Salmonella, Staphylococcus aureus, Shigella flexneri, Listeria monocytogenes, and E. coli O157:H7 recovery from beef and pork [27]. Our study contained a wider variance of enteric bacterial strains and was conducted with human stool specimens opposed to food matrices.…”

Simultaneous Detection of Four Main Foodborne Pathogens in Ready-to-Eat Food by Using a Simple and Rapid Multiplex PCR (mPCR) Assay

“…Among the foodborne outbreaks that represent a cause for concern, most come from animal origin food, including beef meat, poultry, eggs, raw milk and milk products [5][6][7][8], and vegetables such as radishes, cucumbers, carrots, leafy foods such as lettuce, spinach, sprouts, cabbage, or even fruits [9][10][11][12][13][14][15], which may be contaminated by multiple pathogens such as Listeria monocytogenes (L. monocytogenes), Staphylococcus aureus (S. aureus), Salmonella enterica (S. enterica), and Escherichia coli (E. coli) [10,15,16]. Those microorganisms are defined 2 of 18 as significant foodborne pathogen due to the severity of diseases and the number of illness cases caused [15,[17][18][19].…”

“…Unfortunately, the presence of several selective agents may have serious repercussions on the growth balance between the different target pathogens, which can favor one target and inhibit the growth or delay the recovery of the others at the same time [19,33]. In addition, most of those synthetic media developed to be selective, are not specific to the target pathogens [5,11,30,[33][34][35].…”