Development of a monoclonal antibody-based immunoaffinity chromatography and a sensitive immunoassay for detection of spinosyn A in milk, fruits, and vegetables

et al. 2019

Int J of Food Sci Tech

133

Molecular docking is a theoretical simulation method based on bioinformatics, which studies the interaction between molecules (such as ligands and receptors), and predicts their binding modes and affinity via a computer platform. This technology acts as a promising mean in medicinal chemistry such as structurebased rational drug design, which is accepted by researchers in the scientific community. During recent years, various fundamental studies involving biomolecular interaction in the food matrix have gradually emerged. The remarkable advantages of molecular docking such as predicting experiments are attracting increasing attention for its application potential in various fields. This review presents the theory and software development of molecular docking, and emphasises its application in the field of food science, including nutritional components and food safety. Moreover, the operational mechanisms of molecular docking are further summarised in this review.

Section: Food Safety Hazard Factorsmentioning

confidence: 99%

Recent developments in molecular docking technology applied in food science: a review

Tao

Huang

et al. 2019

Int J of Food Sci Tech

133

“…The protocols of immunization and cell fusion were performed as described earlier (Lan et al, 2019). Firstly, 0.5 ml of 1 mg/mL hapten-KLH as immunogen was emulsified with 0.5 mL complete Freund's adjuvant and injected intraperitoneally into five BalB/c mice (0.2 mL for each).…”

Section: Preparation Of Broad-specific Monoclonal Antibodymentioning

confidence: 99%

“…The protocol was performed according to a previous report with some modifications (Lan et al, 2019;Mukunzi, Suryoprabowo, Song, Liu, & Kuang, 2018). Briefly, the microplate was coated with 200 μL hapten-OVA diluted in coating buffer at 37°C for 3 h. The plate was then washed with PBST (0.1% (v/v) Tween-20 diluted in PBS) for 3 times, and blocked by adding non-fat milk/PBS for 30 min.…”

Section: Icelisamentioning

confidence: 99%

Simultaneous detection of carbofuran and 3-hydroxy-carbofuran in vegetables and fruits by broad-specific monoclonal antibody-based ELISA

Lan

Food and Agricultural Immunology

Ding

et al. 2019

Self Cite

Carbofuran is a highly toxic insecticide and has been banned or restricted for pest control in many countries. Being a potent acetylcholinesterase inhibitor, carbofuran and its metabolite 3hydroxycarbofuran (3-OH-CBF) are required for the analysis of their residues in food. However, an immunochemical method for simultaneous analysis of carbofuran and 3-OH-CBF is still not available. Herein, we report an enzyme-linked immunosorbent assay (ELISA) based on a broad-specific monoclonal antibody (mAb) for simultaneous detection of carbofuran and 3-OH-CBF. The mAb, designated as 2E3, against both carbofuran and 3-OH-CBF was used to develop an indirect competitive ELISA (icELISA) with 50% inhibition concentrations of 0.76 and 0.69 ng/mL, respectively. The developed icELISA was validated by UPLC-MS/MS and is suitable for the rapid and simultaneous detection of carbofuran and 3-OH-CBF in fruits and vegetables.

“…Young et al 28 detected SPI residues in 34 matrices using a magnetic particle-based immunoassay test kit, with a limit of quantitation of 0.1–50 ng g −1 . Lan et al 29 developed an ic-ELISA for the detection of spinosyn A in milk, fruits, and vegetables, with a half maximal inhibitory concentration (IC 50 ) of 4.11 ng mL −1 and LOD of 0.63 ng mL −1 . While these methods have adequate sensitivity for the determination of SPI, there are also some limitations.…”

Section: Introductionmentioning

confidence: 99%

Quantitative and rapid detection of spinosad and spinetoram by a gold nanoparticle-based immunostrip

Lei

et al. 2022

Anal. Methods