Development of a Monoclonal Antibody Specific to Cooked Mammalian Meats

Abstract

Detection of species adulteration in ground meat products is important for consumer protection and food-labeling law enforcement. This study was conducted to develop monoclonal antibodies (MAbs) that can be used in an enzyme-linked immunosorbent assay (ELISA) for rapid detection of any cooked mammalian meats in cooked poultry products. Soluble muscle proteins extracted from cooked pork (heated at 100 degrees C for 15 min) were used as the antigen to immunized mice for developing the MAb. One that was developed, MAb 2F8 (IgG2b class), strongly reacted with cooked meat of five mammalian species (beef cattle, hogs, sheep, horse, and deer) but did not react with any cooked poultry (chicken, turkey, and duck) or raw meats. At least 0.5% by weight of pork, beef, lamb, and horse meats in a chicken-based mixture could not detect using the indirect ELISA with MAb 2F8. The MAb 2F8 is useful in a single initial screening test to detect the presence of five nonpoultry meat adulterants in cooked poultry products.

Identification of Different Animal Species in Meat and Meat Products: Trends and Advances

“…These methods have been suggested to resolve proteins of skeletal muscle based on the differences in their isoelectric point or molecular weight (Bauer and Hofmann, 1989;Käuffer et al, 1990;Di Lucciaet al, 1992;Hsieh, 2006) and could be used for mapping of the skeletal muscle proteins of different animal species such as cattle (Bouley et al, 2004;Chaze et al, 2006), swine (Kim et al, 2004;Hollung et al, 2009;Xu et al, 2009), poultry (Doherty et al, 2004) and sheep (Hamelin, 2001). The protein based methods has been reported to be non-suitable for species identification in heated meat products due to denaturation of protein by intensive heating during food processing which in turn lead to modifications in the antigenic activity of molecules and their mobility after electrophoresis ( Jemmi and Schlosser, 1991;Guoli et al, 1999;Giovannacci et al, 2004) consequently, change the ability of antibody to identify its target protein (Owusu-Apenten, 2002), moreover, the possible cross-reaction between closely related species (Hsieh et al, 1998). For these reasons protein-based methods have been replaced by DNA-based ones.…”

Authentication of meat from game and domestic species by SNaPshot minisequencing analysis

“…Those based on protein analysis such as electrophoresis and immunoenzymatic tests (Hsieh, Sheu, & Brifgman, 1998) have been abandoned because of their low specificity (Berger, Mageau, Schwab, & Johnston, 1988). Game species are difficult to discriminate, as they are closely related to each other and are phylogenetically similar to some domestic species.…”

Using species-specific repeat and PCR–RFLP in typing of DNA derived from blood of human and animal species

“…The conventional molecular methods for species identification have been mainly based on protein markers, immunological testing, electrophoresis, high-performance liquid chromatography analysis and DNA hybridization [5][6][7][8][9][10][11]. Application of such protocols has, however, failed to successfully differentiate closely related species, highlighting the need for a method with higher specificity and sensitivity.…”