Development of a monoclonal antibody assay and a lateral flow strip test for the detection of paromomycin residues in food matrices

Abstract

A monoclonal antibody (MAb) was produced against paromomycin and used in the development of an immunoassay and a lateral flow strip test for the detection of paromomycin residues in food matrices. The MAb had 25.1% cross-reactivity with neomycin, but was insensitive to other aminoglycosides. Tested under optimized conditions in 0.01 M phosphate-buffered saline, the half maximum inhibitory concentration (IC 50 ) of the MAb was 0.61 ng/ml for paromomycin and 2.43 ng/ml for neomycin, with results obtained within 90 min. The mean recoveries from spiked food matrices were within the range of 64.56-105.85% for paromomycin and 54.08-100.55% for neomycin. The strip test results for different food matrices were obtained within 5 min and showed visual detection limits of 2.5-20 ng/ml (µg/kg) for paromomycin and 10-30 ng/ml for neomycin. Therefore, the developed immunoassay and strip test can be used in food analysis for routinely monitoring not only paromomycin but also neomycin residues. ARTICLE HISTORY

Development of an immunochromatographic strip test for rapid detection of sodium nifurstyrenate in fish

“…Similarly, few studies have reported NFS determination using high-performance liquid chromatography and/or liquid chromatography tandem mass spectrometry (Horie et al, 1991;Segawa & Ikeda, 2000;Tao et al, 2010). Critically, these methods cannot meet current legislative requirements, due to their relative low sensitivities, their time consuming and expensive, clean-up procedures and their sophisticated analytical instruments (Ge, Suryoprabowo, Zheng, & Kuang, 2017;Isanga et al, 2017;Lin et al, 2018;Liu, Suryoprabowo, Zheng, Song, & Kuang, 2017;Suryoprabowo, Liu, Peng, Kuang, & Xu, 2015).…”

Hapten synthesis and production of specific antibody against 3-amino-5-morpholinomethyl-2-oxazolidone for immunoassay without derivatisation

“…In the development of immunoassays for small organic molecules, as well known, hapten design plays a significant role. However, for some molecules with super-low molecular weight or simple structure, the desired effect cannot be always achieved simply by altering the structure of hapten (Isanga et al, 2017;Luo et al, 2014;Romestand et al, 2010;Xu et al, 2013). At these cases, antibodies were always produced against the derivative but not the small analyte, which means that a pre-derivatisation of the small analyte is necessary prior to analysis.…”

Development of an immunochromatographic test strip for the detection of ochratoxin A in red wine

“…Even though it can simultaneously detect major ochratoxins, HPLC is expensive, time consuming, and unsuitable for on-site detection (Gu, Liu, Song, Kuang, & Xu, 2016;Liu, Suryoprabowo, Zheng, Song, & Kuang, 2017;Suryoprabowo, Liu, Peng, Kuang, & Xu, 2014). TLC, the national standard test method, is rarely used because of its low sensitivity and specificity (Isanga et al, 2017;Khaemba et al, 2016;Suryoprabowo, Liu, Peng, Kuang, & Xu, 2015).…”