Development of an immunochromatographic test strip for the detection of ochratoxin A in red wine

Abstract

Humans and animals are exposed to mycotoxins naturally present in foods. Ochratoxin A (OTA), one of the most abundant and toxic mycotoxins, is ubiquitous in nature and harmful to humans and animals. In this study, we developed a rapid, simple, specific, and sensitive lateral-flow immunochromatographic assay for detection of OTA in 0.01 M phosphate-buffered saline solution (PBS) (pH 7.4) and in red wine. We produced sensitive and selective monoclonal antibody against OTA. Under optimized conditions, the cut-off limit of the test strip was 10 ng/mL OTA in 0.01 M PBS (pH 7.4) and in red wine. The results were obtained within 10 min. Therefore, our developed method is useful for the detection of OTA in red wine. ARTICLE HISTORY

The end user sensor tree: An end-user friendly sensor database

“…This could be altered if a smartphone camera is used as a detector which has been the case for both pesticides (Comina et al, 2016) and mycotoxins (Machado et al, 2018). In fact when reviewing the portable axes of the TEST system, colorimetric techniques currently prevail with microorganisms (Liu et al, 2015) (Wu et al, 2015) (Jiang et al, 2016) and mycotoxins (Zhang et al, 2018) (Liu et al, 2017) (Song et al, 2014) being the most mentioned targets. As for the semi-portable section almost all reports fall in the semi-quantitative group and of these more than half of the reports used a variant of nanotechnology often using EC technology such as Palmsens for detection.…”

Development of an immunochromatographic strip test for rapid detection of sodium nifurstyrenate in fish

“…Enzyme-linked immunosorbent assays are useful in the case when high amounts of samples have to be tested. It has high specificity, requires simple sample preparation steps, and is cost- effective (Lei, Xu, Song, Liu, & Kuang, 2018;Liu, Xu, Suryoprabowo, Song, & Kuang, 2018a;Mukunzi, Suryoprabowo, Song, Liu, & Kuang, 2018;. However, long analysis times are needed to accommodate long incubation times for enzyme-substrate reactions, and unfortunately, this characteristic is not suitable for detection on-site.…”

Ultrasensitive anti-melamine monoclonal antibody and its use in the development of an immunochromatographic strip

“…China urgently needs to carry out high-throughput, rapid detection methods for the detection of melamine residues. Alternative assay methods such as colloidal gold immunoassay strips have the advantages of being rapid, sensitive, and highly efficient, and is therefore suitable for rapid on-site detection of drug residues (Liu, Xu, Suryoprabowo, Song, & Kuang, 2018). In recent years, ICT strips have been reported for the detection of melamine in milk.…”