Development of a molecular method for testing the effectiveness of UV systems on-site

Nizri, Limor; Vaizel-Ohayon, Dalit; Ben-Amram, Hila; Sharaby, Yehonatan; Halpern, Malka; Mamane, Hadas

doi:10.1016/j.watres.2017.10.022

Cited by 8 publications

(6 citation statements)

References 33 publications

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“…Despite the observation in this, and other studies that bacteria are affected differently by UV irradiation, biodosimetry tests are based on single target organisms. This makes it difficult to assess UV irradiation in full scale 21,24,64 . However, in this study families Chitinophagaceae, Pelagibacteraceae, Holophagaceae, Methylophilaceae, and Cytophagaceae showed sensitivity to UV in a linear correlation with dose (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Microbial inactivation of Pseudomonas aeruginosa and Enterococcus faecium by UV was assessed by cultivation and quantitative PCR (qPCR) 19 and impact of UV on adenovirus concentrations were measured by cell culture infectivity and long-range PCR with subsequent qPCR 23 . The impact of UV irradiation on the number of bacteria in drinking water has been quantified with 16s rRNA gene amplification 24 .…”

Section: Introductionmentioning

confidence: 99%

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Impact of UV irradiation at full scale on bacterial communities in drinking water

Pullerits

Ahlinder

Holmer³

et al. 2020

npj Clean Water

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Water in a full-scale drinking water treatment plant was irradiated with ultraviolet (UV) doses of 250, 400, and 600 J/m 2 , and the effect on bacterial communities investigated using 16s rRNA gene amplicon sequencing, heterotrophic plate counts (HPCs), coliform, and Escherichia coli counts. The bacteria in the irradiated water were also analyzed following storage for 6 days at 7°C, to approximate the conditions in the distribution system. The log 10 reduction of HPCs at 400 J/m 2 was 0.43 ± 0.12. Phylogenetic examination, including DESeq2 analysis, showed that Actinobacteria was more resistant to UV irradiation, whereas Bacteroidetes was sensitive to UV. Phylum Proteobacteria contained monophyletic groups that were either sensitive or resistant to UV exposure. The amplicon sequence variants (ASVs) resistant to UV irradiation had a greater average GC content than the ASVs sensitive to UV, at 55% ± 1.7 (n = 19) and 49% ± 2.5 (n = 16), respectively. Families Chitinophagaceae, Pelagibacteraceae, Holophagaceae, Methylophilaceae, and Cytophagaceae decreased linearly in relative abundance, with increasing UV dose (P < 0.05, Pearson's correlation). When irradiated water was stored, Chitinophagaceae, Comamonadaceae, and Flavobacteriaceae families decreased in relative abundance, whereas ACK-M1, Mycobacteriaceae, and Nitrosomonadaceae were increasing in relative abundance. This suggests that the impact of UV irradiation cannot only be considered directly after application but that this treatment step likely continues to influence microbial dynamics throughout the distribution system.npj Clean Water (2020) 3:11 ; https://doi.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Impact of UV irradiation at full scale on bacterial communities in drinking water

Pullerits

Ahlinder

Holmer³

et al. 2020

npj Clean Water

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“…The feasibility of a similar procedure has been demonstrated using PCR to verify the performance of a full scale flow through UV reactor treating groundwater. 26 Whilst the limited number of samples from real treatment works considered during this study are unable to unequivocally demonstrate the robustness of the ELISA, they demonstrate the potential of this approach. The following further work would extend the findings of this work: 1.…”

Section: Discussionmentioning

confidence: 74%

“…PCR based technology has previously been used by several authors to detect UV induced dimerisation in E. coli and P. aeruginosa, and in groundwater samples, 26,27 however PCR detection of dimers relies on a negative response to increasing UV dose and this may influence bias, and limit application to high UV doses. Therefore, a signal based on an increase in response to increasing UV dose may increase the range of doses over which useful information can be obtained and have benefit in addressing bias.…”

Section: Introductionmentioning

confidence: 99%

Immunological detection of thymine dimers in indigenous genomic DNA from pre-disinfection drinking water as an ultraviolet disinfection dosimeter

Blyth

Hazell

Templeton

2021

Environ. Sci.: Water Res. Technol.

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“…48 ATP monitoring has been shown to be useful for monitoring UV disinfection with assay modifications that include a culture step to account for repair. 49 ELISA-based methods 50 and qPCR 51 may also prove useful for monitoring, although molecular methods may require additional refinement to distinguish infective cells from inactivated ones. These data demonstrate the need for tools that accurately quantitate UV disinfection efficacy so that systems can be designed without overdosing that cripples the sustainability of UV disinfection, while also conservatively accounting for repair and regrowth that could negatively impact public and environmental health.…”

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confidence: 99%

Thinking Outside the Treatment Plant: UV for Water Distribution System Disinfection

2019

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This work critically evaluates the current paradigm of water distribution system management and juxtaposes that with the potential benefits of employing UV irradiation, which we hope will catalyze a critical re-evaluation of the current practices in water distribution system management and spur critical research and a new way of thinking about secondary disinfection across the extent of distribution systems. Given the recent advances in UV technology and the efficacy of UV disinfection against all pathogen classes, we now see UV applications for disinfection in many aspects of consumers lives: in water coolers, dishwashers, coffee makers, and disinfection of personal items like gym bags, water bottles, and toothbrushes. Public and regulatory concern over water quality and pathogens, especially the recent interest in building plumbing, calls out for new approaches to disinfection and distribution system management. We envision a new model for secondary disinfection in water distribution systems utilizing emerging germicidal UV LED-based disinfection. UV irradiation in water treatment can achieve high levels of disinfection of all pathogens and minimize or eliminate the formation of regulated disinfection by-products. So why is UV not considered as a secondary disinfectant for distribution systems? In this paper, we lay out the logic as to the benefits and practicality of adding distributed UV treatment to assist in protection of distribution systems and protect water quality for human exposure.

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Development of a molecular method for testing the effectiveness of UV systems on-site

Cited by 8 publications

References 33 publications

Impact of UV irradiation at full scale on bacterial communities in drinking water

Impact of UV irradiation at full scale on bacterial communities in drinking water

Immunological detection of thymine dimers in indigenous genomic DNA from pre-disinfection drinking water as an ultraviolet disinfection dosimeter

Thinking Outside the Treatment Plant: UV for Water Distribution System Disinfection

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