Development of a Molecular Marker for Fruiting Body Pattern in<i>Auricularia auricula-judae</i>

Yao, Fangjie; Lu, Lixin; Wang, Peng; Fang, Ming; Yu, Xiang; Chen, Ying; Zhang, Yan; Kong, Xianghui; Lee, Jia; Honda, Yoshio

doi:10.1080/12298093.2018.1454004

Cited by 19 publications

(15 citation statements)

References 17 publications

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“…Under wild conditions, A. heimuer grows mostly on fallen trees and stumps (Vilela et al 1982), indicating that A. heimuer is a wood-rotting fungus [4]. The sawdust of broad-leaved trees rich in lignin…”

Section: Introductionmentioning

confidence: 99%

Genome Sequence Analysis of Auricularia heimuer Combined with Genetic Linkage Map

Fang

Wang

Chen

et al. 2020

JoF

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Auricularia heimuer is one of the most popular edible fungi in China. In this study, the whole genome of A. heimuer was sequenced on the Illumina HiSeq X system and compared with other mushrooms genomes. As a wood-rotting fungus, a total of 509 carbohydrate-active enzymes (CAZymes) were annotated in order to explore its potential capabilities on wood degradation. The glycoside hydrolases (GH) family genes in the A. heimuer genome were more abundant than the genes in the other 11 mushrooms genomes. The A. heimuer genome contained 102 genes encoding class III, IV, and V ethanol dehydrogenases. Evolutionary analysis based on 562 orthologous single-copy genes from 15 mushrooms showed that Auricularia formed an early independent branch of Agaricomycetes. The mating-type locus of A. heimuer was located on linkage group 8 by genetic linkage analysis. By combining the genome sequence analysis with the genetic linkage map, the mating-type locus of A. heimuer was located on scaffold45 and consisted of two subunits, α and β. Each subunit consisted of a pair of homeodomain mating-type protein genes HD1 and HD2. The mapping revealed conserved synteny at the whole mating-type loci and mirror symmetry relations near the β subunit between A. heimuer and Exidia glandulosa. This study proposed the potential for the bioethanol production by consolidated bioprocessing of A. heimuer. It will promote understanding of the lignocellulose degradation system and facilitate more efficient conversion of the agricultural wastes used for mushroom cultivation. It also will advance the research on the fruiting body development and evolution of A. heimuer.

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Section: Introductionmentioning

confidence: 99%

Genome Sequence Analysis of Auricularia heimuer Combined with Genetic Linkage Map

Fang

Wang

Chen

et al. 2020

JoF

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“…4b, PC1), conforming to a previous report that A . auricula-judae strains with different fruiting body wrinkle characteristics belonged to two major types based on SRAP classification 28 . This observation was further supported by expression patterns of both annotated unigenes (Fig.…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analysis reveals relationship of three major domesticated varieties of Auricularia auricula-judae

Zhao

Wang

Zhang³

et al. 2019

Sci Rep

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Auricularia auricula-judae is an edible mushroom and a traditional medicine in China as well as the fourth largest cultivated mushroom species in the world. Here for the first time, we present comparative transcriptome analyses of the fruiting bodies of three morphologically distinguishable A. auricula-judae cultivated varieties (Wujin, smooth; Banjin, partially wrinkled; and Quanjin, fully wrinkled) collected from Jilin Province, China. Biological triplicates were performed to determine the expression levels of 13,937 unigenes. Among them, only 13 unigenes were annotated to A. auricula-judae, highlighting the lack of publicly available reference sequences for this economically important species. Principal component analysis (PCA) determined that the gene expression profile of Quanjin was unique when compared to those of Banjin and Wujin. Such relationships were further supported by analyses of annotated and unannotated unigenes, differentially expressed unigenes, gene ontology functions, and the family of peroxidase genes. Using the KEGG database, significant alternations in biological pathways were detected among the three cultivars. This work contributes a large set of A. auricula-judae sequences to public database, establishes the relationships among major cultivars, and provides molecular guidance for breeding and cultivation.

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“…SRAP have application in the fields of systematics, conservation, biogeography, and ecology, because they are easy to develop and use, are inexpensive, required small amounts of tissue, and can be used to detect high levels of polymorphism. For fungi, SRAP was initially used to analyze the genetic diversity of Ganoderma strains [ 40 ], then it was applied successfully in the studies of genetic diversity of some edible mushroom [ 13 – 15 , 17 , 18 ]. In this study, the genetic diversity of 72 samples of the wild edible mushroom Lepista nuda from Northeast China was investigated by use of ISSR and SRAP molecular markers.…”

Section: Discussionmentioning

confidence: 99%

“…Quél. [ 13 ], Lentinula edodes (Berk.) Pegler [ 14 ], Pleurotus citrinopileatus Singer [ 15 ], Pleurotus eryngii (DC.)…”

Section: Introductionunclassified

Genetic diversity of Lepista nuda (Agaricales, Basidiomycota) in Northeast China as indicated by SRAP and ISSR markers

Guo

et al. 2018

PLoS ONE

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Lepista nuda is a popular wild edible mushroom that grows in China. In this study, we used ISSR and SRAP molecular markers to analyze the genetic diversity of 72 samples of L. nuda from eight populations in Northeast China. In total, six ISSR primers and five pairs of SRAP primers that produced clear and polymorphic banding profiles were selected for assessing L. nuda genetic diversity. The results revealed a high level of genetic variation among the 72 samples (94.4% polymorphism) but a low degree of gene flow among the populations. Among L. nuda populations, genetic distance was not correlated significantly with geographic distance. The antioxidant activity of the samples from each population was also tested and the result showed that all the selected samples had more than 60% DPPH scavenging activities. Nonetheless, the antioxidant activity diversity is not coincident with both the genetic diversity and the geographic distribution. The results indicate that ISSR and SRAP molecular markers are useful for studying the genetic diversity of L. nuda. The results also suggest that L. nuda populations in Northeast China require protection.

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Development of a Molecular Marker for Fruiting Body Pattern inAuricularia auricula-judae

Cited by 19 publications

References 17 publications

Genome Sequence Analysis of Auricularia heimuer Combined with Genetic Linkage Map

Genome Sequence Analysis of Auricularia heimuer Combined with Genetic Linkage Map

Comparative transcriptome analysis reveals relationship of three major domesticated varieties of Auricularia auricula-judae

Genetic diversity of Lepista nuda (Agaricales, Basidiomycota) in Northeast China as indicated by SRAP and ISSR markers

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