Development of a modified artificial insemination technique combining penile vibration stimulation and the swim-up method in the common marmoset

Takabayashi, Shuji; Suzuki, Yuiko; Katoh, Hideki

doi:10.1016/j.theriogenology.2015.01.017

Cited by 4 publications

(2 citation statements)

References 24 publications

(34 reference statements)

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“…Marmoset semen is often processed using a swim‐up technique due to the low volume of seminal fluid and the presence of coagulum. This method reduces concentration and hinders the capture of a large proportion of sperm in some samples 49 . In the present study, evaluation of sperm kinematics throughout the duration of the sperm swim‐up incubation revealed no changes in overall sperm motility or individual sperm kinematic parameters during the incubation.…”

Section: Discussioncontrasting

confidence: 41%

Comparative computer‐assisted sperm analysis in non‐human primates

Schmidt¹,

Mean²,

Dusek³

et al. 2021

J of Medical Primatology

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Background Biomedical research has recently focused on developing new models of human disease by implementing genome‐editing strategies in non‐human primates (NHPs) to introduce relevant gene mutations. There is a need to establish objective semen evaluation methods to select sires for in vitro fertilization to perform germline editing in embryos. Methods Sperm motility kinematic parameters were evaluated using a computer‐assisted semen analysis (CASA) instrument for rhesus macaques (Macaca mulatta), cynomolgus macaques (Macaca fascicularis), and common marmosets (Callithrix jacchus). Results Normative sperm kinematic parameters were established, revealing differences between marmosets and macaques. The impact of season on rhesus macaque sperm motility was modest, where changes in sperm motility related to season were dependent on the individual male. Conclusions These data provide a baseline of normative kinematic parameters for three captive NHP species, in which implementation of CASA may serve as a tool to evaluate NHP semen quality.

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Section: Discussioncontrasting

confidence: 41%

Comparative computer‐assisted sperm analysis in non‐human primates

Schmidt¹,

Mean²,

Dusek³

et al. 2021

J of Medical Primatology

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“…We were able to achieve an embryo cleavage rate of 80.9% and a transgene expression rate of 89.6% after subzonal injection of the lentiviruses, indicating the efficient integration of these proviruses into the marmoset genome with minimal interference on embryonic viability. In addition, recent progresses made on the adaptation of assisted reproductive techniques for marmoset monkeys, including controlled ovarian stimulation protocols, in vitro maturation and fertilization of oocytes, non-invasive sperm collection, embryo collection from donor females and transfer into recipient females, have strongly facilitated the production of transgenic marmosets and enhanced their value as experimental animal models 23 44 45 46 47 48 49 . By implementing these techniques, we were able to repeatedly recover pre-implantation stage marmoset NAT embryos noninvasively without any residual impact to the donor female’s reproductive health.…”

Section: Discussionmentioning

confidence: 99%

Generation of transgenic marmosets expressing genetically encoded calcium indicators

Park

Zhang

Choi

et al. 2016

Sci Rep

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Chronic monitoring of neuronal activity in the living brain with optical imaging techniques became feasible owing to the continued development of genetically encoded calcium indicators (GECIs). Here we report for the first time the successful generation of transgenic marmosets (Callithrix jacchus), an important nonhuman primate model in neurophysiological research, which were engineered to express the green fluorescent protein (GFP)-based family of GECIs, GCaMP, under control of either the CMV or the hSyn promoter. High titer lentiviral vectors were produced, and injected into embryos collected from donor females. The infected embryos were then transferred to recipient females. Eight transgenic animals were born and shown to have stable and functional GCaMP expression in several different tissues. Germline transmission of the transgene was confirmed in embryos generated from two of the founder transgenic marmosets that reached sexual maturity. These embryos were implanted into six recipient females, three of which became pregnant and are in advanced stages of gestation. We believe these transgenic marmosets will be invaluable non-human primate models in neuroscience, allowing chronic in vivo monitoring of neural activity with functional confocal and multi-photon optical microscopy imaging of intracellular calcium dynamics.

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Normal Clinical and Biological Parameters of the Common Marmoset (Callithrix jacchus)

Kramer

Burns

2019

The Common Marmoset in Captivity and Biomedical Research

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Development of a modified artificial insemination technique combining penile vibration stimulation and the swim-up method in the common marmoset

Cited by 4 publications

References 24 publications

Comparative computer‐assisted sperm analysis in non‐human primates

Comparative computer‐assisted sperm analysis in non‐human primates

Generation of transgenic marmosets expressing genetically encoded calcium indicators

Normal Clinical and Biological Parameters of the Common Marmoset (Callithrix jacchus)

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