Development of a microsatellite marker set applicable to genome-wide screening of cynomolgus monkeys (Macaca fascicularis)

Abstract

To develop a microsatellite marker set applicable to genome-wide screening of cynomolgus monkeys (Macaca fascicularis), 148 microsatellite markers were selected from the human genome database. The polymorphisms and inheritance of PCR products were determined by screening twenty unrelated monkeys and by analysis of three families, respectively. As a result, 106 primers (72%) gave PCR products of the size expected for humans and rhesus monkeys. Among these products, polymorphism and single-gene inheritance in cynomolgus monkeys was observed for 66 markers (62%). The average number of alleles at the 66 polymorphic loci was 5.86 (range 2-10), and average heterozygosity was 0.63 (range 0.10-0.88). This is the first report of microsatellite markers for cynomolgus monkeys. Chromosomal mapping of these markers is now in progress.

Genetic diversity and population structure of long‐tailed macaque (Macaca fascicularis) populations in Peninsular Malaysia

“…All of the loci employed in this study provided robust amplifications of polymorphic bands and were highly informative. The number of alleles and the observed and expected heterozygosity estimates were slightly lower than those reported in other studies .…”

“…Kikuchi et al. selected 148 STR markers from the human genome database to develop a panel of STRs that was applicable for genome‐wide screening of long‐tailed macaques. After the cross‐species amplifications, 66 of 148 of the crosses showed polymorphisms and single‐gene inheritance.…”

“…STR markers isolated in a species can be cross-species amplified in other related species with a variable degree of achievement [11]. Kikuchi et al [21] selected 148 STR markers from the human genome database to develop a panel of STRs that was applicable for genome-wide screening of long-tailed macaques. After the cross-species amplifications, 66 of 148 of the crosses showed polymorphisms and single-gene inheritance.…”

Microsatellite Variation in Two Subspecies of Cynomolgus Monkeys (Macaca fascicularis)

“…In addition, high genetic homogeneity permits the use of fewer subjects and cost-efficient research programs. Microsatellite markers have also been developed for some cynomolgus monkeys [Higashino et al, 2009;Kikuchi et al, 2007]. Assessment of the genetic variability of different subspecies of cynomolgus monkeys is also important in the conservation of genetic resources and inbreeding management.…”

“…In the present study, we selected 26 microsatellite markers from previous reports [Andrade et al, 2004;Kikuchi et al, 2007;Morin et al, 1997;Rogers et al, 2005;Smith et al, 2000]. The polymorphism of all the loci was first genotyped in 12 cynomolgus monkeys.…”