Development of a medium for<i>in vitro</i>culture of<i>Galanthus</i>species based on the mineral composition of bulbs

Staikidou, I.; Selby, Christopher P.; Hanks, Gordon R.

doi:10.1080/14620316.2006.11512100

Cited by 11 publications

(20 citation statements)

References 2 publications

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“…Additionally, along with NRM, NAM contains much more Cu than MS, DKW, and WPM. The benefits of using higher amounts of Cu in the culture medium have been presented for both herbaceous and woody plants (Sahrawat et al, 1999;Nas and Read, 2004a;Staikidou et al, 2006). On the other hand, microbial contaminants may survive in vitro for years and they can hold back or promote culture growth.…”

Section: Discussionmentioning

confidence: 99%

“…A strong species × medium interaction effect on bulblet production was related to important differences between mineral compositions of Galanthus species; this difference suggested that it might be better to calculate separate formulae for individual species (Staikidou et al, 2006). In the current study, the differences in growth among almond cultivars on NAM could be a result of their ability to utilize nutrients.…”

Section: Discussionmentioning

confidence: 99%

“…As a result, to optimize a culture medium numerous studies have been repeated. Some researchers have suggested deciding the level of medium components based on composition of growing whole plants or developing tissues (Litvay et al, 1981;Morard and Henry, 1998;Monteiro et al, 2000;Goncalves et al, 2005;Staikidou et al, 2006;Ashrafi et al, 2010). However, the nutrient contents of growing plants and developing tissues are much influenced by growing conditions (Marschner, 1998).…”

Section: Discussionmentioning

confidence: 99%

“…Nutrient analyses of whole plants (Morard and Henry, 1998), leaves (Monteiro et al, 2000;Goncalves et al, 2005), in vitro-grown shoots (Ashrafi et al, 2010), developing seeds (Litvay et al, 1981), bulbs (Staikidou et al, 2006), and mature seeds (Nas and Read, 2004a) have been used for the formulation of the media.…”

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confidence: 99%

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Shortcut to long-distance developing of a tissue culture medium: micropropagation of mature almond cultivars as a case study

Nas¹,

Bolek²,

Sevgin³

2013

Turk J Bot

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Shortcut to long-distance developing of a tissue culture medium: micropropagation of mature almond cultivars as a case study

Nas¹,

Bolek²,

Sevgin³

2013

Turk J Bot

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“…Several tissue cultures of Amaryllidaceae involve the growth regulators NAA, IAA as auxins and BA as a cytokinin [7,10,27,28]. Auxin/cytokinin combinations involving growth regulators mentioned are potent inducers of embryogenic callus formation and/or mass plant propagation in this family.…”

Section: Discussionmentioning

confidence: 99%

Production and antioxidant capacity of tissue cultures from four Amaryllidaceae species

et al. 2017

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The aim of this study was (i) to produce tissue cultures capable of efficient plant regeneration from European naturally occurring protected and/or pharmacologically important Amaryllidaceae species and (ii) to test them for antioxidant activities in order to select tissue cultures that scavenge efficiently oxygen radicals. Bulb explants were collected from Galanthus woronowii, two Leucojum species, and Sternbergia lutea. Leucojum species were Hungarian isolates. Mostly α-naphthalene acetic acid (NAA) and benzyladenine (BA) were used as growth regulator combinations for the induction and maintenance of tissue cultures and further antioxidant activity studies. Galanthus woronowii and L. vernum cultures produced shoots or whole plants via micropropagation (callus stage was observed only sporadically and callus tissue did not contribute to regeneration), whereas L. aestivum and S. lutea produced efficiently whole plants or multiple shoots via embryogenic calli. Total phenolic content, % inhibition of ABTS radical (ABTS*) cation, and peroxidase activities on native polyacrylamide gels were studied and showed differences between cultures. No relationship could be detected between polyphenol content / radical scavenging capacities and H 2 O 2 reducing enzyme activities. For G. woronowii, S. lutea, and a culture line of L. vernum, polyphenol content and ABTS* cation scavenging activities were high and for G. woronowii, comparable to organs of the native plants used as explant sources. Bulbs of native plants showed low radical scavenging activities in general. For L. vernum and L. aestivum tissue cultures grown in the presence of NAA as the sole growth regulator, ABTS* cation scavenging showed low values. Enzymatic antioxidant (pyrogallol peroxidase) activities were low for all cultures and organs of native plants. This study shows the species conservation value of these cultures and highlights the high antioxidant capacity of G. woronowii and S. lutea, attributed to the presence of non-enzymatic scavengers.

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Regulating plant tissue growth by mineral nutrition

Niedz

Evens

2007

In Vitro Cell.Dev.Biol.-Plant

117

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The objective of this study was to determine if the growth of sweet orange (Citrus sinensis (L.) Osbeck cv. 'Valencia') nonembryogenic callus could be regulated and controlled via the mineral nutrient components of the medium. The 14 salts comprising Murashige and Skoog (MS) basal medium were subdivided into five component groups. These five groups constituted the independent factors in the design. A five-dimensional hypervolume constituted the experimental design space. Design points were selected algorithmically by D-optimality criteria to sample of the design space. Growth of the callus at each design point was measured as % increase of fresh weight at 14 d. An analysis of variance was conducted and a response surface polynomial model generated. Model validation was conducted by mining the polynomial for design points to two regions-"MS-like" growth and MS+25% growth and comparing callus growth to predicted growth. Five of the eight selected MS-like points and three of the six MS+25% growth points validated, indicating regions within the design space where growth was equivalent to MS, but the salt combinations were substantially different from MS, and a smaller region where growth exceeded MS by greater than 25%. NH 4 NO 3 and Fe were identified as important factors affecting callus growth. A second experiment was conducted where NH 4 NO 3 and Fe were varied, thus creating a two-dimensional slice through the region of greatest callus growth and provided increased resolution of the response.

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Development of a medium forin vitroculture ofGalanthusspecies based on the mineral composition of bulbs

Cited by 11 publications

References 2 publications

Shortcut to long-distance developing of a tissue culture medium: micropropagation of mature almond cultivars as a case study

Shortcut to long-distance developing of a tissue culture medium: micropropagation of mature almond cultivars as a case study

Production and antioxidant capacity of tissue cultures from four Amaryllidaceae species

Regulating plant tissue growth by mineral nutrition

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