Development of a low-cost, simple, fast and quantitative lateral-flow immunochromatographic assay (ICA) strip for melatonin in health foods

Abstract

Melatonin (MT) is a hormone secreted by the pineal gland which regulates sleep rhythm and is widely used in health foods. However, MT is sometimes added illegally and excessively in certain health foods, which might cause undesirable side-effects. In this work, an easy-to-use immunochromatographic assay (ICA) based on mouse monoclonal antibody (mAb) for rapid detection of MT was developed for the purpose of safety. Under the optimal conditions, the developed ICA exhibited a visual limit of detection (VLOD) of 10,000 ng/mL and a LOD of 50 ng/mL (strip reader) with the linear range from 50 ng/mL to 5000 ng/mL. Good correlation was obtained between the results of ICA and standard liquid chromatography (LC) in matrix test and blind samples test. The developed ICA can be applied for rapid detection of samples within 30 min, and is potentially an excellent tool for screening of MT in health foods.

Recent Advances in Electrochemical and Optical Sensors for Detecting Tryptophan and Melatonin

“…Recently, an immunochromatographic assay (ICA) was developed for rapid quantitative melatonin detection using AuNPs conjugated to mouse monoclonal antibodies. 171 The ICA yielded a LOD of 0.185 µM as determined by eye, and a LOD of 215.25 nM as determined using a strip reader, for pharmaceutical melatonin samples measured under optimized experimental conditions. The linear detection range was from 215.25 nM to 42 μM, which was comparable to standard liquid chromatography.…”

“…On the other hand, SWV is usually applied in reversible systems and rapid reaction kinetics systems. [171] an electrochemical sensor containing nitrogen-doped ordered mesoporous carbon/Nafion/glassy carbon electrode (GCE) for the highly sensitive and selective detection of tryptophan. Cyclic voltammogram exhibited a linear increase in the oxidation current with tryptophan concentration in the range of 0.5-70.0 μM, with a LOD of 35.0 nM.…”

Development of a colloidal gold strip assay for the detection of total homocysteine in serum samples

“…The LFIA strip was assembled as previously reported. 29 As shown in Fig. 1a, the strip consisted of a PVC backing card, an NC membrane with a test line (T line) and a control line (C line), a release pad, a sample pad, and an absorbent pad.…”

Preparation of Anti-Aristolochic Acid I Monoclonal Antibody and Development of Chemiluminescent Immunoassay and Carbon Dot-Based Fluoroimmunoassay for Sensitive Detection of Aristolochic Acid I

“…The produced artificial antigens were used for animal immunization described in our previous study [32]. The production of mAb was followed by our previous publication [33]. The obtained mAb was purified by protein G and stored at −20 • C.…”