Development of a loop-mediated isothermal amplification method for rapid detection of pigeon circovirus

Tsai, Shinn Shyong; Chang, Yeng Ling; Huang, Yen Li; Liu, Hung-Jen; Ke, Guan M.; Chiou, Chwei Jang; Hsieh, Yao Ching; Chang, Tsung Chou; Cheng, Li Ting; Chuang, Kui-Hao

doi:10.1007/s00705-013-1906-1

Cited by 11 publications

(10 citation statements)

References 23 publications

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“…A variety of methods have been employed to detect PiCV infection in clinical specimens. The original diagnose methods electron microscopy, histology, dot blot hybridization, and in situ hybridization are time consuming [ 8 – 11 ]. The molecular biology techniques, such as the standard PCR [ 10 , 12 ], Real-time PCR [ 13 ], NGS techniques [ 14 ], and the loop-mediated isothermal amplification method [ 11 ], enabled more rapid and accurate detection of PiCV infections.…”

Section: Introductionmentioning

confidence: 99%

Molecular detection and phylogenetic analysis of pigeon circovirus from racing pigeons in Northern China

et al. 2022

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Background Pigeon circovirus (PiCV) infections in pigeons (Columba livia) have been reported worldwide. Currently, pigeon racing is becoming increasingly popular and considered to be a national sport in China, and even, the greatest competitions of racing pigeons are taking place in China. However, there are still no epidemiologic data regarding PiCV infections among racing pigeons in China. The purpose of our study was to provide information of prevalence, genetic variation and evolution of PiCV from racing pigeons in China. Results To trace the prevalence, genetic variation and evolution of PiCV in sick and healthy racing pigeons, 622 samples were collected from 11 provinces or municipalities in China from 2016 to 2019. The results showed that the positive rate of PiCV was 19.3% (120/622) at the sample level and 59.0% (23/39) at the club level, thus suggesting that the virus was prevalent in Chinese racing pigeons. A sequence analysis revealed that the cap genes of the PiCV strains identified in our study displayed a high genetic diversity and shared nucleotide homologies of 71.9%–100% and amino acid homologies of 71.7%–100%. 28 and 36 unique amino acid substitutions were observed in the Cap and Rep proteins derived from our PiCV strains, respectively. A cladogram representation of PiCV strains phylogeny based on 90 cap gene sequences showed that the strains in this study could be further divided into seven clades (A, B, C, E, G, H, and I) and some of them were closely related to worldwide strains from different types of pigeons. A large number of recombination events (31 events) were also detected in the PiCV genomes from Chinese racing pigeons. Conclusions These findings indicate that PiCV strains circulating in China exhibit a high genetic diversity and also contribute to information of prevalence, genetic variation and evolution of PiCV from racing pigeons in China.

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Section: Introductionmentioning

confidence: 99%

Molecular detection and phylogenetic analysis of pigeon circovirus from racing pigeons in Northern China

et al. 2022

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“…Enclosed within the virions are circular genomes of single-stranded DNA, with sizes ranging from 1.8 kb to 2.2 kb according to the species that infect different mammalian hosts. Within the avian hosts, infections of circoviruses have been reported in the goose, canary, pigeon, swan, duck and parrot [ 23 , 24 , 66 , 67 , 68 ]. Circoviruses in avian hosts are identified to cause immunosuppression, making the hosts much more vulnerable to secondary infections and eventually leading to mortality [ 23 , 67 , 68 ].…”

Section: Lamp Application In Screening Of Avian Virusesmentioning

confidence: 99%

“…Within the avian hosts, infections of circoviruses have been reported in the goose, canary, pigeon, swan, duck and parrot [ 23 , 24 , 66 , 67 , 68 ]. Circoviruses in avian hosts are identified to cause immunosuppression, making the hosts much more vulnerable to secondary infections and eventually leading to mortality [ 23 , 67 , 68 ].…”

Section: Lamp Application In Screening Of Avian Virusesmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification (LAMP) as a Promising Point-of-Care Diagnostic Strategy in Avian Virus Research

Padzil

Mariatulqabtiah

Tan

et al. 2021

Animals

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Over the years, development of molecular diagnostics has evolved significantly in the detection of pathogens within humans and their surroundings. Researchers have discovered new species and strains of viruses, while mitigating the viral infections that occur, owing to the accessibility of nucleic acid screening methods such as polymerase chain reaction (PCR), qualitative (real-time) polymerase chain reaction (qPCR) and reverse-transcription qPCR (RT-qPCR). While such molecular detection methods are widely utilized as the benchmark, the invention of isothermal amplifications has also emerged as a reliable tool to improvise on-field diagnosis without dependence on thermocyclers. Among the established isothermal amplification technologies are loop-mediated isothermal amplification (LAMP), recombinant polymerase amplification (RPA), strand displacement activity (SDA), nucleic acid sequence-based amplification (NASBA), helicase-dependent amplification (HDA) and rolling circle amplification (RCA). This review highlights the past research on and future prospects of LAMP, its principles and applications as a promising point-of-care diagnostic method against avian viruses.

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“…For more than two decades, one of the common problems in young racing pigeons is YPDS, which is associated with poor racing performance, and high morbidity and mortality rates among pigeons of age 3 to 20 weeks [5]. The strong immunosuppressive capacity of PiCV infections increases the probability of secondary infections that result in huge losses [6].…”

Section: Introductionmentioning

confidence: 99%

Immunogenicity and Protective Activity of Pigeon Circovirus Recombinant Capsid Protein Virus-Like Particles (PiCV rCap-VLPs) in Pigeons (Columba livia) Experimentally Infected with PiCV

et al. 2021

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Pigeon circovirus (PiCV) is the most recurrent virus diagnosed in pigeons and is among the major causative agents of young pigeon disease syndrome (YPDS). Due to the lack of an established laboratory protocol for PiCV cultivation, development of prophylaxis is hampered. Alternatively, virus-like particles (VLPs), which closely resemble native viruses but lack the viral genetic material, can be generated using a wide range of expression systems and are shown to have strong immunogenicity. Therefore, the use of VLPs provides a promising prospect for vaccine development. In this study, transfected human embryonic kidney (HEK-293) cells, a mammalian expression system, were used to express the PiCV capsid protein (Cap), which is a major component of PiCV and believed to contain antibody epitopes, to obtain self-assembled VLPs. The VLPs were observed to have a spherical morphology with diameters ranging from 12 to 26 nm. Subcutaneous immunization of pigeons with 100 µg PiCV rCap-VLPs supplemented with water-in-oil-in-water (W/O/W) adjuvant induced specific antibodies against PiCV. Observations of the cytokine expression and T-cell proliferation levels in spleen samples showed significantly higher T-cell proliferation and IFN- γ expression in pigeons immunized with VLPs compared to the controls (p < 0.05). Experimentally infected pigeons that were vaccinated with VLPs also showed no detectable viral titer. The results of the current study demonstrated the potential use of PiCV rCap-VLPs as an effective vaccine candidate against PiCV.

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Development of a loop-mediated isothermal amplification method for rapid detection of pigeon circovirus

Cited by 11 publications

References 23 publications

Molecular detection and phylogenetic analysis of pigeon circovirus from racing pigeons in Northern China

Molecular detection and phylogenetic analysis of pigeon circovirus from racing pigeons in Northern China

Loop-Mediated Isothermal Amplification (LAMP) as a Promising Point-of-Care Diagnostic Strategy in Avian Virus Research

Immunogenicity and Protective Activity of Pigeon Circovirus Recombinant Capsid Protein Virus-Like Particles (PiCV rCap-VLPs) in Pigeons (Columba livia) Experimentally Infected with PiCV

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