Loop-Mediated Isothermal Amplification (LAMP) as a Promising Point-of-Care Diagnostic Strategy in Avian Virus Research

Padzil, Faiz; Mariatulqabtiah, Abdul Razak; Tan, Wen Siang; Ho, Ken; Isa, Nurulfiza Mat; Lau, Han Yih; Abu, Jalila; Chuang, Kui-Hao

doi:10.3390/ani12010076

Cited by 18 publications

(21 citation statements)

References 89 publications

(170 reference statements)

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“…Another pair of “cyclic primers” can further accelerate the reaction. In addition to replication activity, polymerases with high chain displacement activity are required for amplification ( Garafutdinov et al, 2020 ; Padzil et al, 2022 ). Liu et al (2022) developed a LAMP method for LM detection using SYTO9 staining and image processing techniques.…”

Section: Molecular Biology Detection Technologymentioning

confidence: 99%

Research progress on detection techniques for point-of-care testing of foodborne pathogens

Zhao

Huang

et al. 2022

Front. Bioeng. Biotechnol.

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The global burden of foodborne disease is enormous and foodborne pathogens are the leading cause of human illnesses. The detection of foodborne pathogenic bacteria has become a research hotspot in recent years. Rapid detection methods based on immunoassay, molecular biology, microfluidic chip, metabolism, biosensor, and mass spectrometry have developed rapidly and become the main methods for the detection of foodborne pathogens. This study reviewed a variety of rapid detection methods in recent years. The research advances are introduced based on the above technical methods for the rapid detection of foodborne pathogenic bacteria. The study also discusses the limitations of existing methods and their advantages and future development direction, to form an overall understanding of the detection methods, and for point-of-care testing (POCT) applications to accurately and rapidly diagnose and control diseases.

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Section: Molecular Biology Detection Technologymentioning

confidence: 99%

Research progress on detection techniques for point-of-care testing of foodborne pathogens

Zhao

Huang

et al. 2022

Front. Bioeng. Biotechnol.

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“…Recent advanced methods in NAATs are based on isothermal amplification (IA), which overcomes the need for thermal cycling given the ability to amplify the sequence of interest at constant temperatures. , By eliminating the need for thermocyclers, amplification techniques are set free from energy-demanding and technologically complex systems, opening the door to POCT and in-field applications. − IA techniques are thus suitable for laboratories lacking expensive PCR equipment and for low-resource settings. In addition, IA methods require less sample and reagent preparation, and the results can be read through different approaches (e.g., turbidimetry, LF), enhancing their accessibility and ease-of-use. ,, …”

Section: Nucleic Acid Amplification Techniquesmentioning

confidence: 99%

“…LAMP was first published in 2000, 108 after being patented, 105 and since then it has been successfully applied in the detection of a variety of pathogens, food control, and cancer-related mutations. 99,100,109 One of its most relevant achievements has been its approval by the WHO as an alternative molecular diagnostics test for pulmonary tuberculosis (TB). 99,110 LAMP consists of a single amplification period from 60 to 65 °C with a turnaround time (TAT) of less than 1 h. LAMP assays require 4 or 6 primers (these include inner and outer primers), which recognize 6−8 different regions of the target.…”

Section: ■ Nucleic Acid Amplification Techniquesmentioning

confidence: 99%

“…LAMP was first published in 2000, after being patented, and since then it has been successfully applied in the detection of a variety of pathogens, food control, and cancer-related mutations. ,, One of its most relevant achievements has been its approval by the WHO as an alternative molecular diagnostics test for pulmonary tuberculosis (TB). , …”

Section: Nucleic Acid Amplification Techniquesmentioning

confidence: 99%

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Toward Integrated Molecular Lateral Flow Diagnostic Tests Using Advanced Micro- and Nanotechnology

2022

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“…At the moment, scientists are discovering and describing more and more new viruses, owing to the accessibility of nucleic acid screening methods such as polymerase chain reaction (PCR), quantitative (real-time) polymerase chain reaction (qPCR) and reverse-transcription qPCR (RT-qPCR). The review from Padzil et al [ 24 ] describes past results on and future trends of major technologies and their principles, benefits and limitations as promising point-of-care diagnostic methodologies against avian viruses.…”

Section: Perspectives Of the “Avian Pathogens” Researchmentioning

confidence: 99%

Avian Pathogens: Editorial and the Perspectives of Research

Sharshov

2022

Microorganisms

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In the last ten years, humanity has faced new challenges in the field of human and animal health, including emerging viral infections. The widespread occurrence of emerging and re-emerging pathogens (e.g., highly pathogenic avian influenza virus (HPAIV), the new COVID-2019 of zoonotic nature, coronaviruses that cause severe acute respiratory syndrome (SARS), and Middle East respiratory syndrome (MERS)) presents a serious threat to humans. Avian pathogens cause devastating economic losses in poultry industries worldwide. Poultry is one of the main sources of protein in Asia. For instance, in China alone, with a population of more than 1.3 billion people, poultry meat consumption has increased to 19.6 kg/year per person (FAO). According to official estimates, this is over 25 million tons per year, or more than 12.7 billion birds. Therefore, poultry farming in Southeast Asia is considered one of the main “vessels” where avian pathogens can evolve. Wild bird migration makes the spread of these pathogens to other regions increasingly likely, as we have seen worldwide with the H5N8 influenza virus. In addition, avian pathogens can cause devastating epizootics among wild birds and threaten the planet’s bioresources. The importance of wild birds as potential vectors of disease has received recent renewed interest, especially in the frame of the global virome/microbiome concept. Understanding the spread of bacterial and viral pathogens in wild birds may serve as a useful model for examining the spread of other disease organisms, both among birds, and from birds to other taxa. This Special Issue aims to summarize recent advances and to attract new research articles that address existing pathogens in poultry and wild birds. It also aims to compare the epidemiological situation across avian pathogens or countries.

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Loop-Mediated Isothermal Amplification (LAMP) as a Promising Point-of-Care Diagnostic Strategy in Avian Virus Research

Cited by 18 publications

References 89 publications

Research progress on detection techniques for point-of-care testing of foodborne pathogens

Research progress on detection techniques for point-of-care testing of foodborne pathogens

Toward Integrated Molecular Lateral Flow Diagnostic Tests Using Advanced Micro- and Nanotechnology

Avian Pathogens: Editorial and the Perspectives of Research

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