Development of a locus-specific marker and localization of the Rysto gene based on linkage to a catalase gene on chromosome XII in the tetraploid potato genome

Abstract

As a result of many decades of potato breeding in Keszthely, Hungary, numerous varieties resistant to the pathogens attacking potato have been developed using wild Solanum species as resistance sources. In order to simplify the time-consuming breeding process, the present research project involved the development of a SCAR marker correlating with the Ry sto gene, originating from the Solanum stoloniferun species and conferring extreme resistance to the potato Y virus (PVY), using sequence information from a RAPD marker linked to the gene. The usefulness of the marker was tested on 21 varieties resistant or susceptible to PVY and it was found to provide efficient identification of genotypes carrying the Ry sto gene. Based on its linkage to a new chromosome-specific anchor marker (Cat-in2), identified by means of the intron-targeting method not previously used in potato research, the Ry sto gene was localized on chromosome XII.

Genetic diversity assessment of bittersweet (Solanum dulcamara, Solanaceae) germplasm using conserved DNA-derived polymorphism and intron-targeting markers

“…This is because both CDDP and IT use primers based on allele sequences of functionally characterised genes, and thus specific banding patterns corresponding to plant phenotypes can be identified. In a previous study using IT markers, Cernák et al (2008) showed that a portion of the catalase gene is linked to Potato virus Y (PVY) resistance and that this gene might play a role in extreme defense mechanisms in solanaceous plants. However, the development of such markers depends on the availability of robust genomic databases.…”

Mapping and DNA sequence characterisation of the Rysto locus conferring extreme virus resistance to potato cultivar ‘White Lady’

“…Molecular markers co-segregating with Ry sto , e.g., STM0003, YES3-3A, Cat-in2 and ST1, were identified earlier [16,31-33,40]. The closest markers to Ry sto in the genetic map of ‘White Lady’ were STM0003 and ST1 in a distance of 2.95 and 0.53 cM, respectively [31].…”

“…andigena ( Ry adg ) and was mapped on chromosome XI [12]. Another one, derived from S. chacoense ( Ry chc ), is located on chromosome IX [13], while the third one, derived from S. stoloniferum ( Ry sto ), was mapped to chromosome XII [14–16]. A gene, designated Y-1 , co-segregating with Ry adg , was cloned and found to be structurally similar to gene N that confers HR to Tobacco mosaic virus (TMV) in Nicotiana spp.…”

Review Article: Molecular Mechanisms of Resistance to Potato virus X and Y in Potato

“…One of them is originated from the wild species S. stoloniferum (gene Ry sto ) and mapped to chromosome XII (Flis et al, 2005;Song et al, 2005;Cernák et al, 2008). Although the Ry sto gene has not been identified yet, it is very probable that, as the majority of characterised viral resistance genes from plants, it belongs to the NBS-LRR class of resistance genes (Gururani et al, 2012).…”