Development of a linear ion trap mass spectrometer capable of analyzing megadalton MALDI ions

Patil, Avinash A; Liu, Zhe-Xuan; Chiu, Yi-Pang; Lại, Thị Khánh Ly; Chou, Szu-Wei; Cheng, Chun-Yen; Su, Wen‐Min; Liao, Hong-Ting; Agcaoili, Justin Benedict A.; Peng, Wen-Ping

doi:10.1016/j.talanta.2023.124555

Cited by 3 publications

(3 citation statements)

References 53 publications

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“…The nondestructive approach gauges image charges as ions traverse a cylindrical charge-sensitive detector. ,,,,, This method readily discerns charges carried by nanometer- and micrometer-sized macroions [e.g., polystyrene (PS) beads, polyethylene glycol, amyloid fibrils, and viruses], typically ranging from ∼400 to ∼2000. Conversely, the destructive method employs a planar charge-sensitive device to gauge image charges upon ion impact. ,,− This method is applied to synthetic PS beads with diameters of 2–15 μm, revealing charge numbers of 1000–60,000, measured individually using a quadrupole ion trap mass analyzer …”

Section: Introductionmentioning

confidence: 99%

“…This detector is successful in nondestructively measuring PS beads and bacterial spores with charges >3000, achieving a detection limit of ±1500 e . In this study, we propose a destructive charge-sensing particle detector (CSPD) to directly and in real-time measure the charges of micrometer-sized particles. ,, The CSPD exhibits a low noise background of ±200 e , approximately 7.5 times lower than that of the PCB charge detector. Moreover, the CSPD was applied to monitor the charge distribution variation of ESI-generated PS beads measuring 0.75 and 3 μm in size and the freeze-dried bacteria, Staphylococcus aureus, Escherichia coli EC11303, and E.…”

Section: Introductionmentioning

confidence: 99%

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Real-Time Monitoring of the Evaporation and Fission of Electrospray-Ionized Polystyrene Beads and Bacterial Pellets at Elevated Temperatures

Liang,

Cua Estayan,

Hsieh

et al. 2024

Anal. Chem.

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This study uses real-time monitoring, at microsecond time scales, with a charge-sensing particle detector to investigate the evaporation and fission processes of methanol/ micrometer-sized polystyrene beads (PS beads) droplets and bacterial particles droplets generated via electrospray ionization (ESI) under elevated temperatures. By incrementally raising capillary temperatures, the solvent, such as methanol on 0.75 μm PS beads, experiences partial evaporation. Further temperature increase induces fission, and methanol molecules continue to evaporate until PS ions are detected after this range. Similar partial evaporation is observed on 3 μm PS beads. However, the shorter period of the fission temperature range is necessary compared to 0.75 μm PS beads. For the spherical-shaped bacterium, Staphylococcus aureus, the desolvation process shows a similar fission period as compared to 0.75 μm PS beads. Comparably, the rodshaped bacteria, Escherichia coli EC11303, and E. coli strain W have shorter fission periods than S. aureus. This research provides insights into the evaporation and fission mechanisms of ESI droplets containing different sizes and shapes of micrometer-sized particles, contributing to a better understanding of gaseous macroion formation.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Real-Time Monitoring of the Evaporation and Fission of Electrospray-Ionized Polystyrene Beads and Bacterial Pellets at Elevated Temperatures

Liang,

Cua Estayan,

Hsieh

et al. 2024

Anal. Chem.

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“…A miniature digital ion trap, driven by a square wave, could analyze ions from 650 to 70,000 Th. − Great efforts have been made to develop particle ion trap mass spectrometers driven by a sinusoidal frequency scanning technique. For example, Peng and coworkers measured the cell and microparticle mass distribution by the use of a high-voltage rf amplifier. − Nie and coworkers described a miniature ion trap mass spectrometer that could measure polystyrene spheres, silica particles, and mice red blood cells . Chen and coworkers realized the detection of biological macromolecules such as IgG and IgA by using a high-voltage rf amplifier with an output of 800 V p‑p over a scanning frequency range from 300 to 100 kHz. , These studies utilized high-voltage op-amps (e.g., PA194 or PA94) as rf amplifiers to drive ion traps, which was beneficial for detecting the mass of a large particle or biological molecule.…”

Section: Introductionmentioning

confidence: 99%

Development of a High-Field “Brick” Mass Spectrometer with Extended Mass Range and Capable of Characterizing Native Proteins

Zhang,

Jia,

Hong

et al. 2023

Anal. Chem.

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One of the main challenges of analyzing intact proteins on an ion trap mass spectrometer is the mass range limitation, especially for miniature mass spectrometers. In this study, a high-field frequency scanning ion trap miniature mass spectrometer, namely the high-field "Brick" mass spectrometer, was developed to analyze intact proteins. A high-voltage broadband radio frequency (rf) amplifier was designed with a maximum output of 900 V p-p over a frequency range of 130−700 kHz. Compared to the 600 V p-p rf amplifier equipped in the conventional "Brick" mass spectrometer, the mass range of the instrument could be extended from 2000 to over 8000 Th. Sensitivity and mass resolution for native protein analyses were also evaluated and compared. Various proteins as well as their mixtures were analyzed on the high-field "Brick" mass spectrometer. The noncovalent interaction between protein−ligand complexes, lysozyme with triN-acetylchitotriose, was also analyzed. In addition, a hybrid ion scan mode was explored to further expand the mass range of the instrument at both low-and high-mass ends. In the hybrid ion scan mode, both rf frequency and amplitude were tuned, and a mass range from 100 to 12,000 Th was realized. As a result, both small drugs and proteins could be analyzed in a single mass scan. As proof-of-concept demonstrations, a mixture of atenolol and bovine serum albuminand oligomers of transferrin were analyzed.

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MALDI sample preparation methods: A mini review

Patil,

Descanzo,

Dhisale

et al. 2024

International Journal of Mass Spectrometry

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Development of a linear ion trap mass spectrometer capable of analyzing megadalton MALDI ions

Cited by 3 publications

References 53 publications

Real-Time Monitoring of the Evaporation and Fission of Electrospray-Ionized Polystyrene Beads and Bacterial Pellets at Elevated Temperatures

Real-Time Monitoring of the Evaporation and Fission of Electrospray-Ionized Polystyrene Beads and Bacterial Pellets at Elevated Temperatures

Development of a High-Field “Brick” Mass Spectrometer with Extended Mass Range and Capable of Characterizing Native Proteins

MALDI sample preparation methods: A mini review

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