Development of a Lab-on-a-Disk Platform with Digital Imaging for Identification and Counting of Parasite Eggs in Human and Animal Stool

Sukas, Sertan; Dorst, Bieke Van; Kryj, Agata; Lagatie, Ole; Malsche, Wim De; Stuyver, Lieven

doi:10.3390/mi10120852

Cited by 20 publications

(17 citation statements)

References 33 publications

(34 reference statements)

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“…A field evaluation of the LOD technology in human samples from Ethiopia revealed successful capture of STH eggs in the image viewing zone of the LOD device even in low infection intensity settings. 43 An almost perfect agreement with Mini-FLOTAC (Kappa score: 0.91) was seen; in fact, more eggs were counted in the LOD technique.…”

Section: Stool Parasite Concentration Methodsmentioning

confidence: 73%

“…This is a microfluidic platform based on both the centrifugation and flotation of eggs for concentration within an imaging zone, followed by image capture. 43 This technique holds potential for point-of-care application, however, adequate training and education of the users and quality management aspects in terms of device calibration and maintenance must be ensured. A field evaluation of the LOD technology in human samples from Ethiopia revealed successful capture of STH eggs in the image viewing zone of the LOD device even in low infection intensity settings.…”

Section: Stool Parasite Concentration Methodsmentioning

confidence: 99%

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Diagnostic Techniques for Soil-Transmitted Helminths – Recent Advances

Khurana¹,

Mewara²

2021

RRTM

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Soil-transmitted helminth (STH) infections (hookworms, Trichuris, Ascaris ) and Strongyloides spp. are associated with a substantial global burden and high morbidity. Sensitive and specific methods for diagnosis of these infections are essential for mapping the burden in communities, accurate assessment of infection levels, to guide interventions and monitoring the success of STH control programs. Despite considerable progress to control STH over several decades, we are still far from identifying a fully adequate diagnostic test. Conventional microscopy-based methods such as direct Kato–Katz smear or mounts after stool centrifugation/flotation-based concentration techniques have been the mainstay of diagnosis, especially in resource-poor countries where these infections abound. However, recently, these are being adapted to closed, easy to perform, digital formats, thereby improving the sensitivity as well as applicability in a remote, resource-limited setting. The use of image analysis systems to identify and quantify helminth eggs, with potential adaptation to smartphones, is also promising. Antibody detection tests have a limited role mostly in the case of Strongyloides hyperinfection. Coproantigen detection tests have been developed and used in veterinary practice for detection of STH, but these have not been evaluated for use in humans. More sensitive molecular diagnostics, including assays developed with new bioinformatic tools and techniques such as polymerase chain reaction (PCR), quantitative PCR (qPCR) and loop-mediated amplification assay, can help in the clear and precise assessment of STH burden during elimination phase and are of immense value for diagnosis in areas with low endemicity and in travelers to endemic regions. Moreover, the molecular techniques will help detect new species that may emerge. Sample preservation and efficient DNA extraction are critical and significantly affect the efficiency of molecular diagnostic tests. In addition to the diagnosis of clinical or asymptomatic infection in humans, detection of STH eggs in environmental samples is imperative to boost STH control efforts. Overall the diagnostic performance, cost-effectiveness, ease of performance, rapidity and in-field applicability of any test should be considered when choosing from the various diagnostic assays in areas with different endemicity, in addition to striving towards the development of novel technologies and optimization of existing methods.

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Section: Stool Parasite Concentration Methodsmentioning

confidence: 73%

Section: Stool Parasite Concentration Methodsmentioning

confidence: 99%

Diagnostic Techniques for Soil-Transmitted Helminths – Recent Advances

Khurana¹,

Mewara²

2021

RRTM

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“…This controversy suggests that the KK procedure is in principle a valid method, but improvements in the readout and reporting procedure could alleviate the shortcomings. The integration of egg detection and readout technologies have been prototyped in, e.g., the FECPAK G2 , KANKANET, spin-disc platforms, and mini-FLOTAC KUBIC [15][16][17][18]. However, the field experience of these technologies provide lessons around complexity and turnaround time as compared to the reference method (Table 2).…”

Section: Low Hanging Fruit To Improve the Current Reference Methodsmentioning

confidence: 99%

The role of diagnostic technologies to measure progress toward WHO 2030 targets for soil-transmitted helminth control programs

Stuyver

Levecke

2021

PLoS Negl Trop Dis

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“…Recently, a LOD device was proposed [ 18 ] for the detection of soil-transmitted helminths (STH) which are intestinal worms that infect humans and are spread through contaminated soil [ 19 , 20 ]. The device represents a centrifugal microfluidic platform based on centrifugation and flotation which isolates and collects eggs within an imaging zone using saturated sodium chloride as flotation solution.…”

Section: Introductionmentioning

confidence: 99%

Migration Behavior of Low-Density Particles in Lab-on-a-Disc Devices: Effect of Walls

et al. 2021

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The effect of the lateral walls of a Lab-On-a-Disc device on the dynamics of a model system of particles with a density lower than that of the solvent (modelling parasites eggs) is analyzed theoretically and experimentally. In the absence of lateral walls, a particle always moves in the direction of the centrifugal force, while its trajectory is deflected in the tangential direction by the inertial Coriolis and Euler forces. Lateral walls, depending on the angle forming with the radial direction, can guide the particle either in the same or in the opposite direction to the centrifugal force, thus resulting in unusual particle trajectories including zig-zag or backwards particle motion. The effect is pronounced in the case of short operation times when the acceleration of the angular rotation, and thus the Euler force, is considerable. The predicted unusual motion is demonstrated by numerically solving the equation of motion in the presence of lateral walls and verified in the experiment with particles of density lower than that of the solvent. Our analysis is useful for design and operational considerations of Lab-On-a-Disc devices aiming for or involving (bio)particle handling.

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Development of a Lab-on-a-Disk Platform with Digital Imaging for Identification and Counting of Parasite Eggs in Human and Animal Stool

Cited by 20 publications

References 33 publications

Diagnostic Techniques for Soil-Transmitted Helminths – Recent Advances

Diagnostic Techniques for Soil-Transmitted Helminths – Recent Advances

The role of diagnostic technologies to measure progress toward WHO 2030 targets for soil-transmitted helminth control programs

Migration Behavior of Low-Density Particles in Lab-on-a-Disc Devices: Effect of Walls

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