2020
DOI: 10.20944/preprints202002.0321.v1
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Development of a Honey Bee RNA Virus Vector Based on the Genome of Deformed Wing Virus

Abstract: We developed a honey bee RNA-virus vector based on the genome of a picorna-like Deformed wing virus (DWV), the main viral pathogen of the honey bee (Apis mellifera). To test the potential of DWV to be utilized as a vector, the 717 nt sequence coding for the enhanced green fluorescent protein (eGFP), flanked by the peptides targeted by viral protease, was inserted into an infectious cDNA clone of DWV in-frame between the leader protein and the virus structural protein VP2 genes. The in vitro RNA transcripts fro… Show more

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Cited by 4 publications
(19 citation statements)
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“…The levels of DWV in these pupae, 10 10 − 10 11 GE ( Fig. 2c), were similar to those observed in the pupae infected with DWV-GFP by injection 40 . Therefore, such injection-infected pupae could be used as an adequate surrogate for Varroa-infected pupae in cannibalism experiments.…”
Section: Trophallactic Transmission Of the Virus Acquired By Pupal Casupporting
confidence: 79%
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“…The levels of DWV in these pupae, 10 10 − 10 11 GE ( Fig. 2c), were similar to those observed in the pupae infected with DWV-GFP by injection 40 . Therefore, such injection-infected pupae could be used as an adequate surrogate for Varroa-infected pupae in cannibalism experiments.…”
Section: Trophallactic Transmission Of the Virus Acquired By Pupal Casupporting
confidence: 79%
“…Experiment A tested if cannibalization of the pupae infected with DWV by Varroa mites could result in development of the virus infection in worker bees. It included injecting honey bee pupae with a ltered tissue extract containing DWV-GFP particles 40 , or by a phosphate buffer saline (PBS) control. After 48 hours (hr), when GFP uorescence had developed in the DWV-GFP-injected pupae indicating virus infection ( Fig.…”
Section: Acquisition Of Dwv By Worker Bees As a Results Of Cannibalismmentioning
confidence: 99%
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