Development of a highly sensitive chemiluminescence enzyme immunoassay using enhanced luminol as substrate

Tao, Xiaoqi; Wang, Wenjun; Wang, Zhanhui; Cao, Xingyuan; Zhu, Jinghui; Niu, Lanlan; Wu, Xiaoping; Jiang, Haiyang; Shen, Jianzhong

doi:10.1002/bio.2544

Cited by 18 publications

(23 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We applied the chemiluminescent detection using luminol as a probe for evaluation H 2 O 2 scavenging capacity of PFCs. The method has been recommended by several groups of researchers as an assay of good selectivity . The mechanism of the CL generated during oxidation of luminol is well characterized, and O • 2 − have been reported as a crucial intermediate in this process .…”

Section: Resultsmentioning

confidence: 99%

“…Effect of PFCs on the CL response of H 2 O 2 ‐induced oxidation of luminol was examined using the CL assay given by Tao et al A reaction mixture was prepared to contain 100 μM of luminol, 5 mM H 2 O 2 , and phosphate buffer pH 7.0. The reaction was initiated by an addition of H 2 O 2 , and 15 sec after the start of the light emission and reaching maximal intensity, 0.5 mM of a test compound DMSO solution or the same volume of alone DMSO was added to the reaction mixture.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Synthesis and in vitro antioxidant activity study of some new piperazinyl flavone compounds

et al. 2017

View full text Add to dashboard Cite

Flavones exhibit a variety of beneficial effects and are well known for their medicinal importance in several diseases, including cardiovascular, neurodegenerative and cancer. The inclusion of the piperazine ring to the flavone backbone is an important strategy in drug discovery but only a few studies have synthesized piperazinyl flavone compounds to test their biological activity. While there is a major focus on the antioxidant properties of drugs in therapy of several diseases of inflammatory origin, we synthesized a series of the novel piperazinyl flavone analogues bearing the phenyl ring with different substituents. The analogues were evaluated for in vitro antioxidant activity against superoxide anion radical, hydroxyl radical, 2,2-diphenyl-1-picrylhydrazyl radical, and hydrogen peroxide scavenging properties. The total antioxidant status based on the absorbance of the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical cation (ABTS ) and total antioxidant capacity using the Fe(III)-ferrozine complex were also monitored. The results of the above studies showed that the compounds synthesized were found possessed moderate radical scavenging potential, and that their interaction with reactive oxygen species is complex and depends on their structural conformation and the type of substituent R in the piperazine ring being attached. Best antiradical activity were found for the compounds with methoxy groups on the phenyl ring of substituent R, whereas the presence of methoxy or trifluoromethyl groups in substituent R resulted in higher ABTS and ion Fe(III) reduction. These compounds are promising molecules to be used for their antioxidant properties and may be regarded, after improvement of the antioxidant potential, to control diseases of free radical etiology.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Synthesis and in vitro antioxidant activity study of some new piperazinyl flavone compounds

et al. 2017

View full text Add to dashboard Cite

show abstract

“…In order to evaluate the scavenger activity of the tested pyruvate derivatives against hydrogen peroxide, a previously described CL method based on H 2 O 2 ‐induced oxidation of luminol was used . The reaction mixture contained different concentrations of the examined compound, 100 μM luminol, phosphate buffer pH 7.0 and 5 mM H 2 O 2 .…”

Section: Methodsmentioning

confidence: 99%

“…Hydrogen peroxide scavenging activity. In order to evaluate the scavenger activity of the tested pyruvate derivatives against hydrogen peroxide, a previously described CL method based on H 2 O 2 -induced oxidation of luminol was used (19 Hydroxyl radical scavenging activity. The Fenton reaction (Fe(II) + H 2 O 2 ) was used as a source of hydroxyl radicals (20,21).…”

Section: Chemiluminescence Measurementsmentioning

confidence: 99%

Reactivity of pyruvic acid and its derivatives towards reactive oxygen species

et al. 2015

View full text Add to dashboard Cite

Pyruvic acid and its derivatives occurring in most biological systems are known to exhibit several pharmacological properties, such as anti-inflammatory, neuroprotective or anticancer, many of which are suggested to originate from their antioxidant and free radical scavenger activity. The therapeutic potential of these compounds is a matter of particular interest, due to their mechanisms of action, particularly their possible antioxidant behaviour. Here, we report the results of a study of the effect of pyruvic acid (PA), ethyl pyruvate (EP) and sodium pyruvate (SP) on reactions generating reactive oxygen species (ROS), such as superoxide anion radicals, hydroxyl radicals and singlet oxygen, and their total antioxidant capacity. Chemiluminescence (CL) and spectrophotometry techniques were employed. The pyruvate analogues studied were found to inhibit the CL signal arising from superoxide anion radicals in a dose-dependent manner with IC50 = 0.0197 ± 0.002 mM for EP and IC50 = 69.2 ± 5.2 mM for PA. These compounds exhibited a dose-dependent decrease in the CL signal of the luminol + H2O2 system over the range 0.5-10 mM with IC50 values of 1.71 ± 0.12 mM for PA, 3.85 ± 0.21 mM for EP and 22.91 ± 1.21 mM for SP. Furthermore, these compounds also inhibited hydroxyl radical-dependent deoxyribose degradation in a dose-dependent manner over the range 0.5-200 mM, with IC50 values of 33.2 ± 0.3 mM for SP, 116.1 ± 6.2 mM for EP and 168.2 ± 6.2 mM for PA. All the examined compounds also showed antioxidant capacity when estimated using the ferric-ferrozine assay. The results suggest that the antioxidant activities of pyruvate derivatives may reflect a direct effect on scavenging ROS and, in part, be responsible for their pharmacological actions.

show abstract

“…Chemiluminescent immunoassays (CLIAs) have some features such as non-radioactive wastes, simple instrumentation, the very low limit of detection (LOD), high sensitivity, wide dynamic range and rapid analysis time in only several seconds. Those characteristics make it broadly used in clinical, environment, food and factory analysis in recent years [14][15].…”

Section: Introductionmentioning

confidence: 99%

Gold Magnetic Nanoparticles-based Chemiluminescent Immunoassay for Detection of Chloramphenicol in Milk

et al. 2016

View full text Add to dashboard Cite

Abstract. chemiluminescent immunoassays (CLIA) based on gold magnetic nanoparticles (Au-MNPs) were developed for rapid analysis of chloramphen icol (CAP) in milk sample. Anti-CAP antibodies were immobilized on the surfaces of Au-MNPs, luminol (Method I) and 2',6'-DiMethylcarbonylphenyl-10-sulfopropylacridinium-9-carboxylate 4'-NHS Ester, (NSP-DMAE-NHS, Method II) were exploited in competitive CLIA for CAP detection in milk using a homemade luminescent measurement system. The sensitivities and limits of detection (LODs) of the two methods were obtained according to the inhibition curves. It indicated that NSP-DMAE-NHS as luminescence reagent (reaction II) was more sensitive and effective than luminol (reaction I). The LOD of reaction II reached 0.008 ng/ml while it was 4 ng/ml in reaction I. Moreover, the linear range of the inhibition curve of the former was wider than that of the latter. Such results indicated that the proposed CLIA stratery employing NSP-DMAE-NHS was more sensitive than other immunoassay method for CAP detection.

show abstract

Development of a highly sensitive chemiluminescence enzyme immunoassay using enhanced luminol as substrate

Cited by 18 publications

References 21 publications

Synthesis and in vitro antioxidant activity study of some new piperazinyl flavone compounds

Synthesis and in vitro antioxidant activity study of some new piperazinyl flavone compounds

Reactivity of pyruvic acid and its derivatives towards reactive oxygen species

Gold Magnetic Nanoparticles-based Chemiluminescent Immunoassay for Detection of Chloramphenicol in Milk

Contact Info

Product

Resources

About