Development of a highly sensitive lateral flow immunoassay based on receptor-antibody-amorphous carbon nanoparticles to detect 22 β-lactams in milk

Zhang, Xiya; Zhao, Fang; Sun, Yi-Ming; Mi, Tiejun; Wang, Liye; Li, Qian; Li, Jiayin; Ma, Wentao; Liu, Wanjing; Zuo, Jingnan; Chu, Xinyu; Chen, Bing; Han, Wenxin; Mao, Yexuan

doi:10.1016/j.snb.2020.128458

Cited by 26 publications

(10 citation statements)

References 22 publications

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“…The bioconjugation protocol by physical adsorption is simpler and does not require an activation phase of the functional groups. Zhang X. et al (2020) obtained carbon nanoparticle-based conjugates by sonicating 1 mg of carbon NPs suspended in 1 ml of 0.01 M borate buffer for 10 min, then adding 16 µg of antibody and incubating for 30 min with slow stirring. 40 µl of blocking buffer containing 20% BSA was added under stirring for 30 min, followed by centrifugation at 8,000 g for 10 min.…”

Section: Conjugation Protocolsmentioning

confidence: 99%

Latest Trends in Lateral Flow Immunoassay (LFIA) Detection Labels and Conjugation Process

Mirica

Stan²,

Chelcea³

et al. 2022

Front. Bioeng. Biotechnol.

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LFIA is one of the most successful analytical methods for various target molecules detection. As a recent example, LFIA tests have played an important role in mitigating the effects of the global pandemic with SARS-COV-2, due to their ability to rapidly detect infected individuals and stop further spreading of the virus. For this reason, researchers around the world have done tremendous efforts to improve their sensibility and specificity. The development of LFIA has many sensitive steps, but some of the most important ones are choosing the proper labeling probes, the functionalization method and the conjugation process. There are a series of labeling probes described in the specialized literature, such as gold nanoparticles (GNP), latex particles (LP), magnetic nanoparticles (MNP), quantum dots (QDs) and more recently carbon, silica and europium nanoparticles. The current review aims to present some of the most recent and promising methods for the functionalization of the labeling probes and the conjugation with biomolecules, such as antibodies and antigens. The last chapter is dedicated to a selection of conjugation protocols, applicable to various types of nanoparticles (GNPs, QDs, magnetic nanoparticles, carbon nanoparticles, silica and europium nanoparticles).

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Section: Conjugation Protocolsmentioning

confidence: 99%

Latest Trends in Lateral Flow Immunoassay (LFIA) Detection Labels and Conjugation Process

Mirica

Stan²,

Chelcea³

et al. 2022

Front. Bioeng. Biotechnol.

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“…The antibody-directed particle accumulation generates a detectable signal on the membrane. There are various types of reporter particles applied in LFAs, such as colloidal gold nanoparticles, latex beads [66], carbon nanoparticles [67][68][69], composite nanoparticles [70], magnetic nanoparticles [71], liposomes [72], fluorescent probes [30,42], and enzymes [73]. However, LFA for phytoproducts shared a common reporter molecule (colloidal gold nanoparticles), whereas a few used quantum dots or carbon nanoparticles as reporters.…”

Section: Reporter For Lfamentioning

confidence: 99%

Lateral flow immunoassay for small-molecules detection in phytoproducts: a review

et al. 2022

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Phytoproducts are involved in various fields of industry. Small-molecule (Mw < 900 Da) organic compounds can be used to indicate the quality of plant samples in the perspective of efficacy by measuring the necessary secondary metabolites and in the perspective of safety by measuring the adulterant level of toxic compounds. The development of reliable detection methods for these compounds in such a complicated matrix is challenging. The lateral flow immunoassay (LFA) is one of the immunoassays well-known for its simplicity, portability, and rapidity. In this review, the general principle, components, format, and application of the LFA for phytoproducts are discussed.

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“…A similar problem can arise in the use of certain animal feeds and environmental medications [ 70 ]. Consequently, the literature contains many microfluidic devices for the detection of antibiotics and drugs, such as ampicillin [ 71 ], β-lactamase [ 72 , 73 ], 17β-estradiol [ 74 ], bacitracin zinc [ 75 ], chloramphenicol [ 76 , 77 ], clarithromycin [ 78 ], clenbuterol [ 79 ], ciprofloxacin [ 80 ], enrofloxacin [ 81 ], monensin [ 82 ], norfloxacin [ 83 ], streptomycin [ 84 ], and tylosin and tilmicosin [ 85 ].…”

Section: Microfluidic Platforms For Milk Sample Analysismentioning

confidence: 99%

“…Zhang et al [ 73 ] developed a microfluidic lab-on-paper lateral flow immunoassay device for the detection of β-lactams in milk samples. As shown in Figure 3 a, the immunoassay platform used amorphous carbon nanoparticles (ACNs) to label anti-receptor monoclonal antibodies (mAb) to form a complex with the unlabeled β-lactam receptors.…”

Section: Microfluidic Platforms For Milk Sample Analysismentioning

confidence: 99%

Recent Advances in Microfluidic Devices for Contamination Detection and Quality Inspection of Milk

Lee

Kung

et al. 2021

Micromachines

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Milk is a necessity for human life. However, it is susceptible to contamination and adulteration. Microfluidic analysis devices have attracted significant attention for the high-throughput quality inspection and contaminant analysis of milk samples in recent years. This review describes the major proposals presented in the literature for the pretreatment, contaminant detection, and quality inspection of milk samples using microfluidic lab-on-a-chip and lab-on-paper platforms in the past five years. The review focuses on the sample separation, sample extraction, and sample preconcentration/amplification steps of the pretreatment process and the determination of aflatoxins, antibiotics, drugs, melamine, and foodborne pathogens in the detection process. Recent proposals for the general quality inspection of milk samples, including the viscosity and presence of adulteration, are also discussed. The review concludes with a brief perspective on the challenges facing the future development of microfluidic devices for the analysis of milk samples in the coming years.

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Development of a highly sensitive lateral flow immunoassay based on receptor-antibody-amorphous carbon nanoparticles to detect 22 β-lactams in milk

Cited by 26 publications

References 22 publications

Latest Trends in Lateral Flow Immunoassay (LFIA) Detection Labels and Conjugation Process

Latest Trends in Lateral Flow Immunoassay (LFIA) Detection Labels and Conjugation Process

Lateral flow immunoassay for small-molecules detection in phytoproducts: a review

Recent Advances in Microfluidic Devices for Contamination Detection and Quality Inspection of Milk

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