Development of a high-throughput method for the determination of ethosuximide in human plasma by liquid chromatography mass spectrometry

Bhatt, Mitesh; Shah, Sanjay; Shivprakash,

doi:10.1016/j.jchromb.2010.03.019

Cited by 13 publications

(2 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further work has shown that this pathway converges in the activation of YAP and that YAP activation is critical for the oncogenic potential of UM (Feng et al, 2014a(Feng et al, , 2014bYu et al, 2014a). The Hippo/YAP cascade is a key growth-regulating pathway in normal cellular physiology (Bhatt et al, 2010;Moroishi et al, 2015;Yu et al, 2015). Unsurprisingly, dysregulation of the Hippo pathway is seen frequently in cancer; however, its core components are rarely mutated (Martin et al, 2018;Moroishi et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

A Platform of Synthetic Lethal Gene Interaction Networks Reveals that the GNAQ Uveal Melanoma Oncogene Controls the Hippo Pathway through FAK

et al. 2019

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

A Platform of Synthetic Lethal Gene Interaction Networks Reveals that the GNAQ Uveal Melanoma Oncogene Controls the Hippo Pathway through FAK

et al. 2019

View full text Add to dashboard Cite

show abstract

“…There are different analytical methods for the quantification of ETX in biological matrix. These methods include gas chromatography (GC) with flame ionization detector or mass spectrometry (MS), high-performance liquid chromatography (HPLC) with Ultra-Violet (UV) or MS, and ultra-performance liquid chromatography (UPLC) with MS. [8][9][10][11][12][13][14] However, most of the published literature deals with the determination of ETX in plasma or serum samples, while few methods have been proposed for the saliva. Among the routine process of bioanalysis, sample preparation is the foremost step in cleaning a complex biological matrix.…”

mentioning

confidence: 99%

Sensitive determination of ethosuximide in human fluids by electromembrane extraction coupled with high performance liquid chromatography ultraviolet spectroscopy

Nabizadeh

Mohammadi

Dolatabadi

et al. 2022

J Chinese Chemical Soc

View full text Add to dashboard Cite

Ethosuximide (ETX) is a common antiepileptic drug in the first line of absence epilepsy. In this study, for the first time, an economical and efficient electro‐membrane (EME) method for determination of ETX in a complex biological matrix using HPLC‐UV has been developed. Factors affecting conventional EME were evaluated. 1‐Octanol was immobilized in a polypropylene membrane and a voltage of 35 V was applied between two platinum electrodes for 15 min. The pH of acceptor and donor phases for ionization of ETX was adjusted to 13 and 11, respectively. Under optimal microextraction conditions, the enrichment factor was 21.02 and the linear range of ETX was 0.25 to 8.00 μg/mL with an acceptable R2 ≥ 0.9986. Inter‐day and intra‐day precision and accuracy of the suggested method were calculated with RSD < 9.5% and relative error <7.0%, respectively. The mean relative recovery of ETX in the human saliva and plasma samples was 81.68% and 74.47, respectively; while limit of detection and quantification concentrations were 0.08 and 0.25 μg/mL, respectively. Furthermore, to evaluate the application of the method, plasma and saliva samples of volunteers administering a single dose of ETX were analyzed successfully by EME‐HPLC‐UV method.

show abstract

Green Chromatography

2012

Handbook of Green Analytical Chemistry

View full text Add to dashboard Cite

Greening the university laboratories 3.2Green laboratory experiments 3.2.1Green methods for sample pretreatment 3.2.2 Green separation using liquid-liquid, solid-phase and solventiess extractions 3.2.3Green alternatives for chemical reactions 3.2.4Green spectroscopy 3.3The place of Green Analytical Chemistry in the future of our laboratories References vi Contents 4 Publishing in Green Analytical Chemistry 55 Salvador G'arrigues and Miguel de la Guardia 4.1 A bibliometric study of the literature in Green Analytical Chemistry 56 4.2 Milestones of the literature on Green Analytical Chemistry 57 4.

show abstract

Development of a high-throughput method for the determination of ethosuximide in human plasma by liquid chromatography mass spectrometry

Cited by 13 publications

References 20 publications

A Platform of Synthetic Lethal Gene Interaction Networks Reveals that the GNAQ Uveal Melanoma Oncogene Controls the Hippo Pathway through FAK

A Platform of Synthetic Lethal Gene Interaction Networks Reveals that the GNAQ Uveal Melanoma Oncogene Controls the Hippo Pathway through FAK

Sensitive determination of ethosuximide in human fluids by electromembrane extraction coupled with high performance liquid chromatography ultraviolet spectroscopy

Green Chromatography

Contact Info

Product

Resources

About