Development of a High-Speed Real-Time Polymerase Chain Reaction System Using a Circulating Water-Based Rapid Heat-Exchange

Terazono, Hideyuki; Hattori, Akira

doi:10.1143/jjap.49.06gm05

Cited by 16 publications

(12 citation statements)

References 12 publications

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“…By using an infrared (IR) laser to irradiate DNA, DNA denaturation can be achieved [16][17][18][19][20] and applied to targetsequence detection. 19 Because lasers enable the rapid heating of substances, they can be employed to induce microdroplet PCR [21][22][23] and to investigate PCR within microchannels. 24 After PCR, the delay before product analysis can affect the target-sequence detection rate.…”

Section: Introductionmentioning

confidence: 99%

Laser-induced heating integrated with a microfluidic platform for real-time DNA replication and detection

Hung

Ho²,

Chen

2016

J. Biomed. Opt

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This study developed a microfluidic platform for replicating and detecting DNA in real time by integrating a laser and a microfluidic device composed of polydimethylsiloxane. The design of the microchannels consisted of a laser-heating area and a detection area. An infrared laser was used as the heating source for DNA replication, and the laser power was adjusted to heat the solutions directly. In addition, strong biotin–avidin binding was used to capture and detect the replicated products. The biotin on one end was bound to avidin and anchored to the surface of the microchannels, whereas the biotin on the other end was bound to the quantum dots (Qdots). The results showed that the fluorescent intensity of the Qdots bound to the replicated products in the detection area increased with the number of thermal cycles created by the laser. When the number of thermal cycles was ≥10, the fluorescent intensity of the Qdots was directly detectable on the surface of the microchannels. The proposed method is more sensitive than detection methods entailing gel electrophoresis.

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Section: Introductionmentioning

confidence: 99%

Laser-induced heating integrated with a microfluidic platform for real-time DNA replication and detection

Hung

Ho²,

Chen

2016

J. Biomed. Opt

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“…We then cultivate and observe the cells under fully controlled conditions (e.g., cell population, network patterns, or nutrient conditions) using an on-chip single-cell cultivation chip (Inoue et al 2001;Inoue et al 2004;Wakamoto et al 2003;Wakamoto et al 2005;Wakamoto and Yasuda 2006;Matsumura et al 2003;Kawai-Noma et al 2006) or an on-chip agarose microchamber system exploiting photo-thermal etching technology, which can control the microstructure of microchambers even during cell cultivation (Moriguchi et al 2002;Hattori et al 2004;Sugio et al 2004;Suzuki et al 2005Suzuki and Yasuda 2007a, b;Kojima et al 2004Kojima et al , 2005Kojima et al , 2006. Finally, we undertake single-cell-based genome/proteome analysis through a set of nanoprobes and adaptive electron microscopy (Kim et al 2010), single-cell-based DNA/RNA release technology (Yasuda et al 2000), or a 3-min ultra-high-speed polymerase chain reaction (PCR) measurement technology (Terazono et al 2010).…”

Section: On-chip Cellomics Technology: Teconstructive Understanding Omentioning

confidence: 99%

Dominant rule of community effect in synchronized beating behavior of cardiomyocyte networks

Yasuda

2020

Biophys Rev

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Exploiting the combination of latest microfabrication technologies and single cell measurement technologies, we can measure the interactions of single cells, and cell networks from "algebraic" and "geometric" perspectives under the full control of their environments and interactions. However, the experimental constructive single cell-based approach still remains the limitations regarding the quality and condition control of those cells. To overcome these limitations, mathematical modeling is one of the most powerful complementary approaches. In this review, we first explain our on-chip experimental methods for constructive approach, and we introduce the results of the "community effect" of beating cardiomyocyte networks as an example of this approach. On-chip analysis revealed that (1) synchronized interbeat intervals (IBIs) of cell networks were followed to the more stable beating cells even their IBIs were slower than the other cells, which is against the conventional faster firing regulation or "overdrive suppression," and (2) fluctuation of IBIs of cardiomyocyte networks decreased according to the increase of the number of connected cells regardless of their geometry. The mathematical simulation of this synchronous behavior of cardiomyocyte networks also fitted well with the experimental results after incorporating the fluctuation-dissipation theorem into the oscillating stochastic phase model, in which the concept of spatially arranged cardiomyocyte networks was involved. The constructive experiments and mathematical modeling indicated the dominant rule of synchronization behavior of beating cardiomyocyte networks is a kind of stabilityoriented synchronization phenomenon as the "community effect" or a fluctuation-dissipation phenomenon. Finally, as a practical application of this approach, the predictive cardiotoxicity is introduced.

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“…Recently, systems using hot and cool water for heat transfer through metal blocks have achieved 5.25-and 4.6-s cycles (8,9 ). Infrared-mediated temperature cycling demonstrated 3-s cycles (10 ); vapor pressure-augmented, continuous-flow PCR, 3-s cycles (11 ); and PCR within glass capillaries submerged in heated and cooled liquid gallium, 2.7-s cycles (12 ).…”

confidence: 99%

Extreme PCR: Efficient and Specific DNA Amplification in 15–60 Seconds

2015

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Development of a High-Speed Real-Time Polymerase Chain Reaction System Using a Circulating Water-Based Rapid Heat-Exchange

Cited by 16 publications

References 12 publications

Laser-induced heating integrated with a microfluidic platform for real-time DNA replication and detection

Laser-induced heating integrated with a microfluidic platform for real-time DNA replication and detection

Dominant rule of community effect in synchronized beating behavior of cardiomyocyte networks

Extreme PCR: Efficient and Specific DNA Amplification in 15–60 Seconds

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