Development of A High-Speed, Multiplexed Sample-Delivery Instrument For Lc–MS/MS Bioanalysis

Abstract

Development of the sample delivery platform and its application in high-throughput and gradient LC will be described.

A high‐speed liquid chromatography/tandem mass spectrometry platform using multiplexed multiple‐injection chromatography controlled by single software and its application in discovery ADME screening

“…The developed system and methods using multiplexed LC coupled with MIC data acquisition significantly reduced the injection‐to‐injection cycle time (23 s) than our previously reported platform. This cycle time approaches those reported for the new ADDA system and RapidFire system, with both running direct online SPE in MIC mode. In comparison, advantages of this platform reported herein include its relatively lower cost, its compatibility with various LC and MS components from major vendors, and its single point software control by Aria.…”

“…One additional complicating factor for ADME bioanalytical platforms is the integration with hardware/software solution for automated SRM method optimization, as that is the pre‐requisite for SRM quantitation of a large number of compounds being profiled. The recently introduced Apricot Designs Dual Arm (ADDA) is the first commercially available system with all these features by integrating with DiscoveryQuant (DQ), a software tool by AB Sciex, to enable streamlined compound optimization, acquisition method creation and sample submission …”

High-Throughput Mass Spectrometry for Hit Identification: Current Landscape and Future Perspectives

“…The bottom traces represent time events in the staggered parallel separation. Reproduced with permission from de Biasi et al 10 and Wu, 12 respectively.…”

Acoustic Ejection/Full-Scan Mass Spectrometry Analysis for High-Throughput Compound Quality Control

“…8 In an effort to minimize MS idle time, the multiplexed LC approach combined with multiple injection chromatography for data acquisition yields a typical analysis speed of 0.5 min per sample. 9,10 An even faster injection-to-injection cycle time of 5–10 s has been achieved on the RapidFire system (Agilent Technologies, Santa Clara, CA), where the chromatographic separation is simply reduced to a minimum of online solid-phase extraction. 11,12 Sample pooling for cassette LC-MS analysis has also been seen for high-throughput nanoscale synthesis 3 and various in vitro profiling assays 13,14 to alleviate the analytical workload.…”