Development of a High-Performance Liquid Chromatography Method for Quantification of Fluazuron in Cattle Tissues

Zhang, Yumei; Lu, Xinlun; Hu, Chenyun; Cui, Weibo; Liu, Xue

doi:10.1080/10826076.2012.723090

Cited by 2 publications

(1 citation statement)

References 4 publications

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“…Steroids, such as estradiol and progesterone, are related to follicle growth and atresia, also having an effect on oocytes. In fact, the FF has a variety of functions related to the oocyte including maintaining meiotic arrest, protection against proteolysis, extrusion during ovulation and protection against adverse blood influences (SOHEL & CINAR, 2014) and, therefore, based on their importance physiological and the fact that residues of veterinary drugs can be found in various tissues (XIN-LUN et al, 2013;ZHANG et al, 2013), this research was conducted, however, without success in detection.…”

Section: Journal Of Agricultural Studiesmentioning

confidence: 99%

Fluazuron Influences the in vitro Production Embryos of Wagyu Cow

Silva

Cavalieri

Andreazzi

et al. 2022

JAS

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Imported breeds raised in tropical countries demand greater use of chemical control of ectoparasites, however, this practice can compromise the results of biotechnologies of reproduction. Thus, the objective of this study was to evaluate the presence of chemical residues of tick or their metabolites in the blood and in the follicular fluid and to verify the in vitro embryo production (IVEP) of Wagyu cows, raised in a tropical country, submitted to fluazuron based tick treatment. Twenty adult Wagyu bovine females were used, donors of oocytes, divided into 2 groups: G1 - animals that were not submitted to tick control and G2, animals that were submitted to chemical control of ticks with fluazuron based product (2.5 mg kg-1 of body weight). After application (D0), all cows were submitted to estrous synchronization and, in four moments (D12, D33, D54 and D75) the aspirations of the follicular fluid from the dominant follicles were performed, the oocytes were collected for IVEP and were collected blood samples for extraction and analysis of the presence of chemical residues, using GC-MS. Plasma residues of fluazuron were detected up to 54 days after application of the tick, but no residues were detected in the follicular fluid. Group 1 had a higher number of total and viable oocytes (p<0.0001), however, the viability rate and the blastocyst rate was lower (p<0.0001), showing that the use of ticks compromised IVEP.

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Section: Journal Of Agricultural Studiesmentioning

confidence: 99%

Fluazuron Influences the in vitro Production Embryos of Wagyu Cow

Silva

Cavalieri

Andreazzi

et al. 2022

JAS

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Bioanalytical Method to Measure Fluazuron in Bovine Plasma and its Application in Pharmacokinetic Studies

Ferreira¹,

Lima²,

Magalhães³

et al. 2019

Rev. Virtual Quim

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Método Bioanalítico para o Fluazuron em Plasma Bovino e Sua Aplicação em Estudos de FarmacocinéticaResumo: Fluazuron é um regulador de crescimento de insetos pertencente à classe da benzofeniluréia que é comercializado para o controle do carrapato bovino. Embora os reguladores de crescimento sejam uma classe amplamente estudada, o número de artigos descrevendo métodos analíticos para determinar o fluazuron em amostras de plasma animal é reduzido. Para avaliar a característica farmacocinética do fluazuron em bovinos, foi avaliado e validado um método CL-UV. As condições experimentais foram otimizadas pelo estudo comparativo de três métodos de extração: ELL, EFS e DMFS. A separação cromatográfica foi realizada na coluna Kromasil C18, com fase móvel de acetonitrila: água (80:20, v / v) na vazão de 1,0 mL/min-1. O método foi validado de acordo com as exigências da ANVISA dentro da faixa de 20 a 150 ng mL-1 (r > 0,99). O LD e LIQ para o fluazuron foram 10 e 20 ng mL -1 no plasma. A precisão e exatidão inter / intra-ensaio foram ≤ 9,48 % (CV) e ≤ 10,3 % (EPR), respectivamente. Como resultado, foram obtidos parâmetros farmacocinéticos de C max (74,4 ± 3,52 ng mL -1 ), AUC 0-t (1007 ± 33,5 ng*d mL -1 ) e t 1/ 2 (14,6 ± 2,97 d) para fluazuron após administração tópica em bovinos. O método bioanalítico foi adequado para a aplicação nos estudos de farmacocinética, uma vez que os valores de LIQ para o fluazuron permitiram que a quantificação atingisse a AUC 0-t superior a 80 % da AUC 0-∞.

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Development of a High-Performance Liquid Chromatography Method for Quantification of Fluazuron in Cattle Tissues

Cited by 2 publications

References 4 publications

Fluazuron Influences the in vitro Production Embryos of Wagyu Cow

Fluazuron Influences the in vitro Production Embryos of Wagyu Cow

Bioanalytical Method to Measure Fluazuron in Bovine Plasma and its Application in Pharmacokinetic Studies

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