Development of a gold nanoparticle-based strip assay for detection of clopidol in the chicken

Chao, Mengjia; Liu, Liqiang; Song, Shanshan; Wu, Xiaoling; Kuang, Hua

doi:10.1080/09540105.2020.1737655

Cited by 10 publications

(7 citation statements)

References 29 publications

(19 reference statements)

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“…To meet the needs of on-the-spot, rapid, low-cost, and accurate detection, immunological detection methods have become the main research focus. The existing reports of immunological detection methods include gold nanoparticle-based strips ( 25 ), gold nanoparticle-based lateral-flow strips ( 29 , 30 ), enzyme-linked immunoassays ( 24 ), and high efficiency chemiluminescent immunoassays ( 15 ). The accuracy and stability of immunological methods mainly depend on the antibody sensitivity.…”

Section: Resultsmentioning

confidence: 99%

Generation and Characterization of an Anti-diclazuril Monoclonal Antibody and Development of a Diagnostic Enzyme-Linked Immunosorbent Assay for Poultry

Shen

Sun

et al. 2022

Front. Nutr.

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An anti-diclazuril monoclonal antibody (mAb) was developed for use in enzyme-linked immunosorbent assay (ELISA)-based detection of diclazuril with high sensitivity and specificity, which can be used to measure anti-coccidial drug residues. The anti-diclazuril mAb had a half-maximal inhibitory concentration of 0.449–0.517 ng/mL. The mAb cross-reactivity with toltrazuril, toltrazuril 18 sulfone, clozaril, monesin, madurmycin, and salinomycin was very minimal (< 0.1%). The detection limit of the ELISA using this mAb was 0.10 ng/mL and the sensitivity was 0.05 ng/mL. A standard curve generated in the range of 0.05–16.2 ng/mL had a linear correlation coefficient value of ≥ 0.99. The average recoveries of diclazuril from chicken and duck samples ranged from 85.0 to 102.5%.Intra- and inter-assay coefficients of variation ranged from 5.9 to 8.5% and 9.2 to 12.6%, respectively. Using the International Immunogenetics Information System®, the VH domain of the mAb was found to be encoded by an IGHV3 family gene and had the following complementarity determining region (CDR) sequences: GFTFSRY (CDR1), SRGGS (CDR2), and GDDNYAFAY (CDR3). The VL domain was encoded by an IGKV1 family gene and had the following CDR sequences: KSSQSLLNSRTRKNYLA (CDR1), WASTRES (CDR2), and KQSYNLHT (CDR3). This study provides a method to generate anti-diclazuril mAbs and determine their variable region sequences. The diagnostic ELISA developed using this mAb may drive additional studies on the monitoring and detection of food and veterinary drug residues.

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Section: Resultsmentioning

confidence: 99%

Generation and Characterization of an Anti-diclazuril Monoclonal Antibody and Development of a Diagnostic Enzyme-Linked Immunosorbent Assay for Poultry

Shen

Sun

et al. 2022

Front. Nutr.

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“…GNPs were prepared by reduction with sodium citrate as previously described in our laboratory. , One percent sodium citrate solution was added to chloroauric acid solution and stirred quickly until the mixture turned a fuchsia color. Then, the mixture was stored at 4 °C for future use.…”

Section: Methodsmentioning

confidence: 99%

Sensitive Lateral Flow Immunoassay for the Residues of Imidocarb in Milk and Beef Samples

Wang

Guo

et al. 2021

ACS Omega

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Based on the successful derivation of a hapten, we prepared and optimized a murine monoclonal antibody against imidocarb, with an IC 50 of 2.22 ng/mL and a limit of detection (LOD) of 0.45 ng/mL. Cross-experiment results showed that the cross-over rate for 4,4′-dinitrocarbanilide was 18.12%, and the cross-reactivity with other analogues when using the ic ELISA was less than 0.1%. We used the developed ic-ELISA to detect the addition and recovery of imidocarb in milk and beef samples, and values were 86.0−93.5 and 84.5−101.2%, respectively. The preparation of an immunochromatographic test strip based on gold nanoparticles was used for the rapid identification of imidocarb in milk and beef samples. When assessed by the naked eye, the visual LOD for imidocarb in milk and beef samples was 5 and 10 ng/mL, and the cut-off values were 20 and 50 ng/mL, respectively. Because of its high sensitivity, specificity, and simplicity, the test strip can be used for on-site testing and rapid screening of imidocarb in food samples.

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“…The resulting ascites were collected and purified as previously described. [35][36][37] Finally, the isolated mAbs were stored at −20 °C.…”

Section: Production Of Mabsmentioning

confidence: 99%

A gold-based immunochromatographic strip for the detection of sirolimus in human whole blood

Jiang

Zeng

et al. 2022

Analyst

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Development of a gold nanoparticle-based strip assay for detection of clopidol in the chicken

Cited by 10 publications

References 29 publications

Generation and Characterization of an Anti-diclazuril Monoclonal Antibody and Development of a Diagnostic Enzyme-Linked Immunosorbent Assay for Poultry

Generation and Characterization of an Anti-diclazuril Monoclonal Antibody and Development of a Diagnostic Enzyme-Linked Immunosorbent Assay for Poultry

Sensitive Lateral Flow Immunoassay for the Residues of Imidocarb in Milk and Beef Samples

A gold-based immunochromatographic strip for the detection of sirolimus in human whole blood

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