Sensitive Lateral Flow Immunoassay for the Residues of Imidocarb in Milk and Beef Samples

Abstract

Based on the successful derivation of a hapten, we prepared and optimized a murine monoclonal antibody against imidocarb, with an IC 50 of 2.22 ng/mL and a limit of detection (LOD) of 0.45 ng/mL. Cross-experiment results showed that the cross-over rate for 4,4′-dinitrocarbanilide was 18.12%, and the cross-reactivity with other analogues when using the ic ELISA was less than 0.1%. We used the developed ic-ELISA to detect the addition and recovery of imidocarb in milk and beef samples, and values were 86.0−93.5 and 84.5−101.2%, respectively. The preparation of an immunochromatographic test strip based on gold nanoparticles was used for the rapid identification of imidocarb in milk and beef samples. When assessed by the naked eye, the visual LOD for imidocarb in milk and beef samples was 5 and 10 ng/mL, and the cut-off values were 20 and 50 ng/mL, respectively. Because of its high sensitivity, specificity, and simplicity, the test strip can be used for on-site testing and rapid screening of imidocarb in food samples.

Tailor-Made Immunochromatographic Test for the Detection of Multiple 17α-Methylated Anabolics in Dietary Supplements

“…The visual detectability of the developed methods is comparable to other ICTs for the analysis of low molecular weight compounds such as methiocarb, thiabendazole, forchlorfenuron, robenidine or imidocarb [21,36,[44][45][46]. At the same time, the obtained detection limit is sufficient for the detection of commonly used concentrations of AASs for the illegal fortification of dietary supplements.…”

An ultrasensitive NIR-IIa’ fluorescence-based multiplex immunochromatographic strip test platform for antibiotic residues detection in milk samples

“…The specificity of NIR-II-FNPs-based IST platform was evaluated by cross-activity rate of analytes (SA, QN, and LIN) with six other kinds of common antibiotic residues in animal body fluids, including aflatoxin B1, tetracycline, erythromycin, melamine, gentamicin, and chloramphenicol. Cross-activity rate (%) of analyte against each other antibiotic residue was calculated using [27] : Cross-activity rate (%) = (IC 50 of analyte/IC 50 of other antibiotic residue) × 100 %. …”

A gold-based immunochromatographic strip for the detection of sirolimus in human whole blood

“…In recent years, immunoassays have become very popular for both food 18 and drug 19 monitoring; these techniques include chemiluminescent enzyme immunoassays (CLEIAs), 20 chemiluminescence microparticle immunoassays (CMIAs), 21 colloidal gold immunochromatographic test strips 22,23 and indirect competitive enzyme-linked immunosorbent assays (ic-ELISAs). 24,25 Recently a type of chemiluminescent immunoassay using a polyclonal antibody against sirolimus has been reported. 26 However, compared to monoclonal antibodies (mAbs), its stability was worse.…”