Development of a glucose sensor employing quick and easy modification method with mediator for altering electron acceptor preference

Hatada, Mika; Loew, Noya; Inose-Takahashi, Yuka; Okuda-Shimazaki, Junko; Tsugawa, Wakako; Mulchandani, Ashok; Sode, Koji

doi:10.1016/j.bioelechem.2018.02.001

Cited by 49 publications

(20 citation statements)

References 28 publications

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“…We previously reported that MTT alone did not function as the electron acceptor for a variety of redox enzymes. However, the presence of PMS facilitated the reduction of MTT by the oxidation substrate [26]. Considering that both PMS and MTT are negatively charged in their oxidative status, we assumed that the low reactivity of MTT as the primary electron acceptor might be attributed to its bulkiness when compared with PMS.…”

Section: Investigation Of the Availability Of Wild-type Aspergillus Nmentioning

confidence: 99%

“…Therefore, we have used the availability of MTT as the sole electron acceptor in the solution (MTT system) as the indication that PES was successively modified on the surface of redox enzymes, where internal electron transfer from a deeply buried cofactor to PES occurred. Consequently, bulky MTT can be reduced [26]. Since GOx was revealed that it did not utilize MTT as the primary electron acceptor, we followed our previous empirical experimental condition to analyze the modification of PES on the surface of GOx and evaluate the availability of the arPES modification procedure to prepare quasi-DET type GOx.…”

Section: Investigation Of the Availability Of Wild-type Aspergillus Nmentioning

confidence: 99%

“…GOxs (wild-type and mutant) were modified using arPES, as previously described [26] with some changes. GOx and arPES were mixed in 20 mM tricine buffer (pH 8.3) to 13 µM (based on catalytic centers) and 2.0 mM, respectively, and incubated at 25 • C for 2 h while shaking at 1200 rpm.…”

Section: Pes Modification Of Enzymesmentioning

confidence: 99%

“…In addition, no report has demonstrated the rational design of residues where redox mediators should be modified on GOx. To achieve a versatile and simple method for enzyme modification, we previously reported a rapid and single-step conjugation using 1-[3-(succinimidyloxycarbonyl)propoxy]-5-ethylphenazinium trifluoromethanesulfonate (or amine-reactive phenazine ethosulfate; arPES) [26,27] onto the surface of the redox enzyme. The attachment of these redox mediators to redox enzymes enables intramolecular electron transfer from the enzyme redox center to the mediator and intermolecular electron transfer from the mediator to an electrode.…”

Section: Introductionmentioning

confidence: 99%

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Engineered Glucose Oxidase Capable of Quasi-Direct Electron Transfer after a Quick-and-Easy Modification with a Mediator

Suzuki

Lee

Loew

et al. 2020

IJMS

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Glucose oxidase (GOx) has been widely utilized for monitoring glycemic levels due to its availability, high activity, and specificity toward glucose. Among the three generations of electrochemical glucose sensor principles, direct electron transfer (DET)-based third-generation sensors are considered the ideal principle since the measurements can be carried out in the absence of a free redox mediator in the solution without the impact of oxygen and at a low enough potential for amperometric measurement to avoid the effect of electrochemically active interferences. However, natural GOx is not capable of DET. Therefore, a simple and rapid strategy to create DET-capable GOx is desired. In this study, we designed engineered GOx, which was made readily available for single-step modification with a redox mediator (phenazine ethosulfate, PES) on its surface via a lysine residue rationally introduced into the enzyme. Thus, PES-modified engineered GOx showed a quasi-DET response upon the addition of glucose. This strategy and the obtained results will contribute to the further development of quasi-DET GOx-based glucose monitoring dedicated to precise and accurate glycemic control for diabetic patient care.

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Section: Investigation Of the Availability Of Wild-type Aspergillus Nmentioning

confidence: 99%

Section: Investigation Of the Availability Of Wild-type Aspergillus Nmentioning

confidence: 99%

Section: Pes Modification Of Enzymesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Engineered Glucose Oxidase Capable of Quasi-Direct Electron Transfer after a Quick-and-Easy Modification with a Mediator

Suzuki

Lee

Loew

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…In electrochemical measurements, an electrode mediator or a modified electrode surface is generally required. A variety of electron mediators and electron conductive polymers have been employed [28][29][30][31][32], among which PMS has been found to be the most efficient [26]. Despite the popularity of electrochemical sensors, different shortcomings of these devices are still persistent, like leakage of the enzyme from immobilized layers or denaturation of the enzyme on the electrode surface.…”

Section: Introductionmentioning

confidence: 99%

Pyrroloquinoline quinone glucose dehydrogenase adopted in thermometric analysis for enhancement of glucose determination

Xie

Bülow

Xie

2018

J Therm Anal Calorim

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A broad measurement range of glucose is often required in clinical analysis, especially for diabetic patients where glucose levels can be very high. Pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) has previously been used in electrochemical quantification of glucose with an extended linear range. However, in real sample determination of glucose, interferences from electroactive substances in blood are unavoidable. Calorimetric biosensors, e.g., the Enzyme Thermistor, are insensitive to either directly electroactive or optical interferences often present in real clinical samples. This paper describes a novel analytical strategy where the intrinsic advantages of PQQGDH are combined with the Enzyme Thermistor as biosensor using calorimetric detection as general measurement principle. When compared with the most frequently used enzyme glucose oxidase, PQQGDH has a higher catalytic efficiency and is insensitive to the availability of oxygen. The use of calorimetry in this context resulted in a broad linear range of glucose measurements, from 0.009 to 100 mM, an excellent specificity and insignificant side effects of compounds present in blood at high concentrations, such as lactate and urea.

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Light‐Addressable Amperometric Sensor with Counter and Working Electrodes of the Same Material

Kosugi

Hasegawa

Uchida

2021

IEEJ Transactions Elec Engng

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High‐throughput screening is important in various fields, and electrochemical approaches are effective because of advantages such as fast response time and electrode array. Hence, we developed a two‐dimensional electrochemical array sensor. However, its signal intensities for lower concentrations of the redox pair (100 μM–100 pM [Fe[CN]6]3−/4−) were unstable; Therefore, in this study, we investigated and demonstrated a method for improving the signal stability at lower concentrations. Two types of counter electrodes were prepared to assess their influence; the material of the first electrode was the same as that of the working electrode (same material condition), and the other was prepared from a different material (different material condition). First, the differences in the electric potentials between different materials were investigated at lower concentrations. Furthermore, the concentration dependence was investigated using our newly developed light‐addressable amperometric sensor for the two conditions. In the different condition, it was observed that differences in the electric potentials between the electrodes at lower concentrations. It was also observed that the signal intensity was more stable for the same material condition than the different material condition. Limit of detection(LOD) for the same material condition was lower than that under the different material condition. The proposed method using the same materials is thus an effective means to measure low concentrations of redox ions and expected to contribute to a fabrication of a smaller and more stable device without reference electrode. © 2021 Institute of Electrical Engineers of Japan. Published by Wiley Periodicals LLC.

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Development of a glucose sensor employing quick and easy modification method with mediator for altering electron acceptor preference

Cited by 49 publications

References 28 publications

Engineered Glucose Oxidase Capable of Quasi-Direct Electron Transfer after a Quick-and-Easy Modification with a Mediator

Engineered Glucose Oxidase Capable of Quasi-Direct Electron Transfer after a Quick-and-Easy Modification with a Mediator

Pyrroloquinoline quinone glucose dehydrogenase adopted in thermometric analysis for enhancement of glucose determination

Light‐Addressable Amperometric Sensor with Counter and Working Electrodes of the Same Material

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