Development of a Genetic Linkage Map for Genes Associated With Resistance and Susceptibility to Fusarium Oxysporum F. Sp. Cubense From an F1 Hybrid Population of Musa Acuminata Ssp. Malaccensis

Kayat, Fatimah; Bonar, N.; Waugh, Robert; Rajinder, S.; Rahimah, A. R.; Rashid, Kamaludin; Othman, Rofina Yasmin

doi:10.17660/actahortic.2009.828.34

Cited by 12 publications

(9 citation statements)

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“…However, DH ‘Pahang’ has never been used for in mapping studies; thus, there is a possibility that this reference banana may not even have the resistance gene(s) identified in this study. Kayat et al ( 2009 ) used progenies of Mam generated at the University of Malaya for AFLP-based mapping analysis. However, this was unsuccessful due to the limited progeny size.…”

Section: Discussionmentioning

confidence: 99%

“…Thus far, advanced genetic analyses have mostly focused on TR4 (Fraser-Smith et al 2016 ; Kayat et al 2004 , 2009 ; Peraza-Echeverria et al 2009 ). Kayat et al ( 2009 ) used AFLP markers, and Fraser-Smith et al ( 2016 ) used a selfed Mam pollination, but both groups never provided mapping data on TR4 resistance. After the preparative studies of Peraza-Echeverria et al ( 2008 ), Dale et al ( 2017 ) cloned RGA2 which provided resistance to TR4 that was validated by transferring it to the Cavendish cultivar ‘Grand Nain’.…”

Section: Discussionmentioning

confidence: 99%

“…Faure et al ( 1993 ) built such a genetic map from an F 2 population of two wild M. acuminata varieties based on RFLP, isozyme and RAPD markers, demonstrating 36% segregation distortion of the male-specific alleles. The linkage maps reported by Kayat et al ( 2009 ) were based on two F 1 populations of selfed Mam using AFLP, STMS and RAPD markers, resulting in 32 and 37 linkage groups, many more than the expected 11 groups of diploid banana (2 n = 2 x = 22). Kilian et al ( 2012 ) generated a large number of markers by Diversity Array Technology (DArT) that were used by Hippolyte et al ( 2010 ) in combination with SSR markers to build a linkage map from F 1 progeny of a hybrid between M. acuminata ‘Borneo’ and ‘Pisang Lilin’ that resulted in the expected 11 linkage groups.…”

Section: Introductionmentioning

confidence: 99%

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Genetic mapping of Fusarium wilt resistance in a wild banana Musa acuminata ssp. malaccensis accession

et al. 2020

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Banana is an important fruit and food crop, but is threatened by Fusarium wilt, one of the most devastating soil-borne fungal diseases. Only host resistance facilitates banana cultivation in infested soils around the world, but the genetic basis of Fusarium wilt of banana (FWB) is unknown. We selfed a heterozygous wild banana accession Musa acuminata ssp. malaccensis (Mam, AA, 2n = 22) to generate a mapping population and to investigate the inheritance of resistance to Race 1 and tropical race 4 (TR4) that cause FWB. Phenotyping (N = 217) revealed segregation for resistance, and genotyping by sequencing resulted in 2802 high-quality single-nucleotide polymorphic markers (SNPs) that were used for genetic mapping. Combined analyses of these data showed that a single dominant resistance locus controls resistance to Race 1 and maps near the distal part of chromosome 10. Recombinants, together with the position of the putative resistance gene, were further analysed using graphical genotyping, which retrieved markers flanking a 360 kb genetic region that associates with Race 1 resistance. The region contains 165 putative genes on the reference genome, including 19 leucine-rich repeat receptor-like kinase-like genes. At the same position and phase, we also identified a QTL for TR4 resistance, showing that the locus for resistance against Race 1 provided partial resistance to TR4. However, this effect was far less significant and hence not included in the mapping. These data support the breeding of new banana varieties with resistance to Fusarium wilt.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic mapping of Fusarium wilt resistance in a wild banana Musa acuminata ssp. malaccensis accession

et al. 2020

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“…Pisang liar Musa acuminata var. malaccensis diketahui merupakan salah satu jenis yang tahan terhadap penyakit layu Fusarium (Kayat et al, 2009), sedangkan pisang liar M. acuminata var. zebrina dan M. acuminata var.…”

Section: Penyakitunclassified

“…Pengujian pada pisang liar M. acuminata var. malaccensis menunjukkan hasil yang berbeda dibandingkan penelitian yang telah dilakukan oleh Javed et al (2004) dan Kayat et al (2009) (Poerba et al, 2017). Ketahanan pisang Rejang#2 terhadap Foc TR4 diduga karena tanaman mempunyai suatu gen atau mekanisme ketahanan tertentu (Sutanto et al, 2011) atau adanya simbiosis dengan cendawan endofit yang dapat menghambat pertumbuhan Foc dengan cara memproduksi metabolit volatil atau bukan volatil atau keduanya (Suciatmih et al, 2014).…”

Section: Hasil Dan Pembahasanunclassified

Deteksi Awal Ketahanan Beberapa Aksesi Pisang Lokal dan Hasil Persilangan terhadap Penyakit Layu Fusarium (Foc VCG 01213/16 TR4)

Handayani¹,

Martanti²,

Poerba³

et al. 2018

J Hort Indonesia

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ABSTRACTEarly detection for Fusarium wilt resistant of banana plants can be done at seedling phase under greenhouse condition. The purpose of early detection was to screen and evaluate some number of local and hybrid accession of banana plants for resistance to Fusarium oxysporum f. sp. cubense (Foc VCG 01213/16 TR4 or Foc TR4). The treatments were done by immersing the root seedling in Fusarium inoculant at concentration of 106 conidia mL-1, for 2 hours, then planted in sterile sand media and placed in container with "double tray system". Experiments were performed using 15 accessions of banana i.e. 1 accession of wild musa (Musa acuminata var. malaccesis), 7 local accessions and 7 hybrid bananas. Based on greenhouse bioassay, banana Rejang#2 (AA) showed resistance to Foc TR4. The results were consistent in the field condition. Tetraploid plants for pisang Rejang#2 (AAAA) also showed resistant to Foc TR4. Cultivar Cavendish (AAA) and Madu (AA) showed highly susceptible; Ustrali (AAAB) and Goroho (AAA) showed susceptible to Foc TR4. Wild banana (Musa acuminata var. malaccensis) and Mas Jambe 4x (AAAA) showed moderate susceptible. Of the total 7 hybrids accession; 3 hybrids showed moderate susceptible, and 4 other hybrids showed susceptible to Foc TR4.Keywords: banana plants, early detection, Foc TR4, hybrid, local ABSTRAK Deteksi awal tingkat ketahananan tanaman pisang terhadap penyakit layu Fusarium oxysporum f. sp. cubense (Foc) dapat dilakukan pada masa pembibitan di rumah kaca. Penelitian bertujuan untuk evaluasi awal tingkat ketahanan beberapa aksesi pisang liar, pisang lokal dan pisang hasil persilangan terhadap penyakit layu Fusarium. Akar bibit pisang pada air yang berisi inokulan Fusarium (Foc VCG 01213/16 atau Foc TR4) pada konsentrasi 106 konidia mL-1, selama 2 jam yang selanjutnya ditanam pada media pasir steril dan diletakkan pada kontainer dengan pengaturan “double tray system”. Lima belas aksesi pisang yang terdiri atas 1 aksesi pisang liar (Musa acuminata var. malaccensis), 7 aksesi pisang budidaya dan 7 nomor pisang hasil persilangan dievaluasi tingkat ketahanan terhadap Foc TR4. Dari hasil pengujian di rumah kaca, pisang Rejang#2 (AA) menunjukkan status tahan terhadap penyakit layu Fusarium Foc VCG 01213/16 TR4 yang hasilnya sesuai dan stabil dengan tingkat ketahanannya di lapangan. Pisang Rejang#2 tetraploid (AAAA) juga menunjukkan status tahan terhadap Foc TR4. Pisang Madu (AA) dan Cavendish (AAA) menunjukkan hasil sangat rentan, sedangkan pisang Goroho (AAA) dan pisang Ustrali (AAAB) menunjukkan status rentan. Pisang liar Musa acuminata var. malaccensis (AA) dan pisang Mas Jambe 4x (AAAA) menunjukkan status agak rentan. Pada seleksi pisang hasil persilangan, didapatkan 3 nomor pisang hibrid dengan status agak rentan yakni 816 MDRK, 1060 MDRK, 2284 MMRK, sedangkan 4 nomor hibrid lainnya menunjukkan status rentan.Kata kunci: deteksi dini, Foc TR4, hibrid, lokal, pisang

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Genomic Designing for Biotic Stress Resistant Banana

Backiyarani

Anuradha

Uma

2022

Genomic Designing for Biotic Stress Resistant Fruit Crops

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Development of a Genetic Linkage Map for Genes Associated With Resistance and Susceptibility to Fusarium Oxysporum F. Sp. Cubense From an F1 Hybrid Population of Musa Acuminata Ssp. Malaccensis

Cited by 12 publications

References 0 publications

Genetic mapping of Fusarium wilt resistance in a wild banana Musa acuminata ssp. malaccensis accession

Genetic mapping of Fusarium wilt resistance in a wild banana Musa acuminata ssp. malaccensis accession

Deteksi Awal Ketahanan Beberapa Aksesi Pisang Lokal dan Hasil Persilangan terhadap Penyakit Layu Fusarium (Foc VCG 01213/16 TR4)

Genomic Designing for Biotic Stress Resistant Banana

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