Development of a faster determination of 10 anabolic steroids residues in animal muscle tissues by liquid chromatography tandem mass spectrometry

Ultra-high performance liquid chromatography–tandem mass spectrometry in high-throughput confirmation and quantification of 34 anabolic steroids in bovine muscle

“…These high S/N values provide evidence that detection and confirmation is surely possible at or below the CCˇvalues. Compared to values reported in literature for a smaller number of analytes [18][19][20][21][22], the calculated CC˛and CCˇvalues from this study are equal (in most cases) indicating high sensitivity of the reported methodology. In some cases higher values are reported but this may be explained by the higher ALs applied for these compounds, in line with the Belgian National Reference Laboratory's recommendations and the fact that the signal relative standard deviation ((R.S.D.)…”

“…1). This gradient allowed quantitative determination of a much higher number of steroids in meat than so far reported in literature [18][19][20][21][22].…”

“…versus the analyte concentrations. The estimated noise average of the pool of blanks (n = 20) was used as a forced intercept [20]. Good linearity was obtained; all correlation coefficients (R 2 ) were ≥0.990.…”

“…10.006 other matrices. Several methods on the determination of anabolic steroids in muscular tissue of animal origin have been reported [18][19][20][21][22], monitoring a selection of the existing anabolic steroids (10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20), sometimes in combination with corticosteroids.…”

Natural Hormones in Food-Producing Animals:Legal Measurements and Analytical Implications

“…Although normally the levels of steroids that accumulate in animal tissues are lower than in other matrices, many effective methods are known currently for the determination of these compounds in muscle tissue, sometimes monitoring a wide range of anabolic compounds (Courant et al, 2008;Kaklamanos et al, 2007;Vanhaecke et al, 2011;Yang et al, 2009). Since the number of growth promoters is high and includes natural and synthetic compounds, the use of multianalyte techniques is becoming more interesting (Vanhaecke et al, 2011;Xu et al, 2006;Yang et al, 2009). The use of ultra-resolution liquid chromatography techniques (UPLC), coupled to mass spectrometry devices, provides a rapid separation of analytes, shortening analytical times and improving the simultaneous detection of multiple steroids (Stolker et al, 2008;Vanhaecke et al, 2011).…”