Development of a fast capillary electrophoresis method for the determination of propranolol—Total analysis time reduction strategies

Micke, Gustavo Amadeu; Costa, Ana Carolina Oliveira; Heller, Melina; Barcellos, Michelle; Piovezan, Marcel; Caon, Thiago; Oliveira, Marcone Augusto Leal de

doi:10.1016/j.chroma.2009.08.083

Cited by 25 publications

(14 citation statements)

References 17 publications

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“…The detection limit was better than those of previous reports (from 5.1 to 4.97 mg/L). [1][2][3][4][5][6][7][8][9][10] The relative standard deviations (RSD) of the ECL intensity and the migration time for six consecutive injections of 1.0 μg/mL PRO were 2.6 and 2.1%, respectively.…”

Section: Linearity Detection Limit and Reproducibilitymentioning

confidence: 99%

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Pharmacokinetics of Propranolol Hydrochlorid in Human Urine by Capillary Electrophoresis Coupled with Electrochemiluminescence

et al. 2011

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Section: Linearity Detection Limit and Reproducibilitymentioning

confidence: 99%

“…1,2 Owing to its sedative effect due to reducing cardiac frequency and contraction force, it has been used as a doping agent in sports. The International Olympic Committee has forbidden it in some events such as shooting and weightlifting.…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetics of Propranolol Hydrochlorid in Human Urine by Capillary Electrophoresis Coupled with Electrochemiluminescence

et al. 2011

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“…[5][6][7] PRO and 4'OH PH exhibit natural fluorescence properties, permitting the use of HPLC methods with fluorescence detectors for both compounds. Other methods that have been reported include capillary electrophoresis, [8][9][10] spectrofluorimetric 11,12 and electrochemical methods. [13][14][15] A polarographic method was used to determine the quantity of PRO in pharmaceutical formulations.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical behavior of Propranolol and its major metabolites, 4'-hydroxypropranolol and 4'-hydroxypropranolol Sulfate, on glassy carbon electrode

Baranowska¹,

Koper²

2011

J. Braz. Chem. Soc.

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Foi estudado o comportamento eletroquímico do propranolol (PRO) e seus principais metabólitos. Medidas voltamétricas foram realizadas em eletrodo de carbono vítreo (GCE). Todos os compostos examinados sofrem oxidação via eletroquímica. Em todos os casos foi observada uma separação dos picos de oxidação, sugerindo a possibilidade de eletroanálise simultânea de propranolol e seus metabólitos. A linearidade das curvas de calibração foi obtida para concentrações entre 4,22×10 -6 -1,35×10 -4 mol L -1 para PRO, 4,00×10 -6 -4,81×10 -5 mol L -1 para 4'-hidroxipropranolol (4'OH PH) e 3,52×10 -6 -4,22×10 -5 mol L -1 para o sulfato de 4'-hidroxipropranolol (4'OH PS). O método descrito é rápido e simples, e pode ser aplicado para determinar os compostos mencionados acima em amostras biológicas. Os resultados obtidos foram verificados por cromatografia líquida de ultra-alta eficiência (ultra HPLC).The electrochemical behavior of propranolol (PRO) and its major metabolites was studied. Voltammetric measurements were made on glassy carbon electrode (GCE). All examined compounds were electrochemically oxidized. In all cases, a separation of oxidation peaks was observed, suggesting the possibility of simultaneous electroanalysis of propranolol and its metabolites. The linearity of the calibration curves was obtained for concentrations between 4.22×10 -6 -1.35×10 -4 mol L -1 for PRO, 4.00×10 -6 -4.81×10 -5 mol L -1 for 4'-hydroxypropranolol (4'OH PH) and 3.52×10 -6 -4.22×10 -5 mol L -1 for 4'-hydroxypropranolol sulfate (4'OH PS). The described method is rapid and simple, and could be applied to determine the above mentioned compounds in biological samples. The obtained results were verified by ultra-high performance liquid chromatography (Ultra HPLC) method.

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“…Several methods have been reported for the determination of these drugs which includes spectrophotometry [10][11][12], spectrofluorimetry [13][14], gas chromatography-mass spectrometry (GC-MS) [15][16], high-performance liquid chromatography (HPLC) [17][18], liquid chromatography-mass spectrometry (LC-MS) [19][20][21], chemiluminescence [22][23], capillary electrophoresis [24][25] and voltammetry [26][27]. Spectrophotometric methods because of their inherent simplicity and rapidity of the procedure and low cost of the techniques find favour in most laboratories of limited means and consequently these methods continue to flourish.…”

Section: Introductionmentioning

confidence: 99%

Spectrophotometric Determination of Propranolol Hydrochloride and Metoprolol Tartrate in Pharmaceutical Dosage Forms, Spiked Water and Biological Fluids

Sharma

Singh

Raj

2018

Int J Pharm Pharm Sci

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Objective: A new spectrophotometric method for the determination of propranolol hydrochloride (PRO) and metoprolol tartrate (MTP), beta blocker drugs, has been developed for their analysis in pharmaceutical dosage forms for the purpose of quality control and water samples for monitoring impact on environmental water quality of natural sources and in biological fluids for ascertaining their physiological performance. Methods:The method is based on the derivatization of the amino function present in these drugs to the corresponding yellow copper (I) drug dithiocarbamate derivative through reaction with carbon disulphide, pyridine and copper (I) perchlorate in aqueous acetonitrile and measuring absorbance at 406 nm for propranolol and 400 nm for metoprolol. The different experimental parameters affecting the development and stability of the colour were carefully studied and optimized.Results: The Beer's law is obeyed in the range of 1.0-40.0 μg/ml of each drug solution with a correlation coefficient 0.999. The maximum relative standard deviations (RSDs) in the analysis of pure PRO and MTP were 1.01 and 1.52 % respectively. The recoveries of the drugs from pharmaceutical formulations, spiked water samples and biological fluids were in the range 98.0-100.5 % with RSDs in the range 0.23-1.94% indicating good accuracy and precision of the method. Conclusion:The instantaneous development of colour and its stability, well-established stoichiometry of the reaction and above simplicity and rapidity of procedures are some special attributes of the proposed method.

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Development of a fast capillary electrophoresis method for the determination of propranolol—Total analysis time reduction strategies

Cited by 25 publications

References 17 publications

Pharmacokinetics of Propranolol Hydrochlorid in Human Urine by Capillary Electrophoresis Coupled with Electrochemiluminescence

Pharmacokinetics of Propranolol Hydrochlorid in Human Urine by Capillary Electrophoresis Coupled with Electrochemiluminescence

Electrochemical behavior of Propranolol and its major metabolites, 4'-hydroxypropranolol and 4'-hydroxypropranolol Sulfate, on glassy carbon electrode

Spectrophotometric Determination of Propranolol Hydrochloride and Metoprolol Tartrate in Pharmaceutical Dosage Forms, Spiked Water and Biological Fluids

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