Development of a duplex lateral flow dipstick test for the detection and differentiation of Listeria spp. and Listeria monocytogenes in meat products based on loop-mediated isothermal amplification

Ledlod, Sudarat; Bunroddith, Kespunyavee; Areekit, Supatra; Santiwatanakul, Somchai; Chansiri, Kosum

doi:10.1016/j.jchromb.2019.121834

Cited by 22 publications

(14 citation statements)

References 38 publications

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“…In optimal situations, the method LODs regarding pure culture and genomic DNA have been 20 CFU/mL and 900 femtograms (fg), respectively. The LAMP-DLFD approach has not demonstrated any cross-reactivity with 18 other pathogenic bacteria including S. aureus, Salmonella spp., Campylobacter coli, E. coli, Enterococcus faecalis, Lactobacillus aci-dophilus, V. cholerae, Pseudomonas aeruginosa, V. parahaemolyticus, C. jejuni, Shigella dysenteriae, B. cereus, S. flexneri, L. casei, and Pediococcus pentosaceus (Ledlod et al, 2020).…”

Section: Listeriamentioning

confidence: 99%

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State of the art: Lateral flow assays toward the point‐of‐care foodborne pathogenic bacteria detection in food samples

Sohrabi

Majidi

Khaki

et al. 2022

Comp Rev Food Sci Food Safe

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Diverse chemicals and some physical phenomena recently introduced in nanotechnology have enabled scientists to develop useful devices in the field of food sciences. Concerning such developments, detecting foodborne pathogenic bacteria is now an important issue. These kinds of bacteria species have demonstrated severe health effects after consuming foods and high mortality related to acute cases. The most leading path of intoxication and infection has been through food matrices. Hence, quick recognition of foodborne bacteria agents at low concentrations has been required in current diagnostics. Lateral flow assays (LFAs) are one of the urgent and prevalently applied quick recognition methods that have been settled for recognizing diverse types of analytes. Thus, the present review has stressed on latest developments in LFAs‐based platforms to detect various foodborne pathogenic bacteria such as Salmonella, Listeria, Escherichia coli, Brucella, Shigella, Staphylococcus aureus, Clostridium botulinum, and Vibrio cholera. Proper prominence has been given on exactly how the labels, detection elements, or procedures have affected recent developments in the evaluation of diverse bacteria using LFAs. Additionally, the modifications in assays specificity and sensitivity consistent with applied food processing techniques have been discussed. Finally, a conclusion has been drawn for highlighting the main challenges confronted through this method and offered a view and insight of thoughts for its further development in the future.

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Section: Listeriamentioning

confidence: 99%

“…aureus , Salmonella spp. , Campylobacter coli, E. coli, Enterococcus faecalis, Lactobacillus acidophilus, V. cholerae, Pseudomonas aeruginosa, V. parahaemolyticus, C. jejuni, Shigella dysenteriae, B. cereus, S. flexneri, L. casei , and Pediococcus pentosaceus (Ledlod et al., 2020).…”

Section: Determination Of Pathogenic Bacteria Using Lfasmentioning

confidence: 99%

State of the art: Lateral flow assays toward the point‐of‐care foodborne pathogenic bacteria detection in food samples

Sohrabi

Majidi

Khaki

et al. 2022

Comp Rev Food Sci Food Safe

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“…Labeled primers produce labeled amplification products, which are trapped by a biotin ligand and bound to an LF test strip. In recent studies, LAMP-LF has gained recognition in the detection of various microorganisms and substances (Liu et al 2019;Ledlod et al 2020;Allgöwer et al 2020;Jalandra et al 2020), but the use of this technology to accurately detect E. coli of serotype O157:H7 has not been reported to date. In this study, based on the z3276 genetic marker, we developed a z3276-LAMP-LF assay, combining the LAMP method with an LF test strip, which enabled faster and more specific identification of E. coli O157:H7 with a lower detection limit than previously established methods.…”

Section: Introductionmentioning

confidence: 99%

Rapid Detection of Escherichia coli O157:H7 by Loop-Mediated Isothermal Amplification Coupled with a Lateral Flow Assay Targeting the z3276 Genetic Marker

et al. 2021

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The most commonly reported serotype of Shiga toxin-producing Escherichia coli (STEC) is O157:H7. This pathogen presents a threat to public health and is a cause of foodborne illness worldwide. The efficient and sensitive detection of E. coli O157:H7 remains a challenge for food safety. In this report, we developed a sensitive and specific loop-mediated isothermal amplification (LAMP) reaction coupled with a lateral flow (LF) assay to rapidly detect E. coli O157:H7. Six specific primers were targeted to the genetic marker z3276. Two loop primers were labeled with either fluorescein isothiocyanate (FITC) or digoxin (Dig), which facilitated analysis by the LF assay. The LAMP-LF assay detected E. coli O157:H7, and no crossreactivity was observed with the other bacterial strains tested, including non-O157 STEC and other E. coli. The detection limit of the LAMP-LF assay was 1.3 CFU/mL for E. coli O157:H7 in broth culture, which was of equal or higher sensitivity than the other amplicon detection methods tested (agarose electrophoresis and dye). Analysis of samples from slaughterhouses indicted that the z3276-LAMP-LF assay was superior in terms of sensitivity, accuracy, and rapidity in detecting E. coli O157:H7 compared with conventional PCR. A further advantage of this method is that it is not dependent upon specialized equipment or techniques and therefore has wide potential applications in the field.

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“…The test results can be obtained at least 3-5 days after the entire process has been completed. Molecular biological detection methods for pathogens have been widely studied and applied (2)(3)(4)(5)(6); however, these require professional expertise and expensive experimental equipment. This is not conducive to the rapid detection of Salmonella or the identification of infections when there is a shortage of personnel and equipment, or at primary medical institutions, incident scenes, and disaster areas.…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of Salmonella based on loop-mediated isothermal amplification

Wang

Gao

et al. 2021

Ann Palliat Med

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Background: Salmonella enterica is a zoonotic pathogen of substantial concern to human and animal health and is a leading cause of morbidity and mortality in people worldwide. Loop-mediated isothermal amplification (LAMP) technology is a new type of nucleic acid amplification technology, which has the characteristics of high specificity, high sensitivity, simple operation, convenience, and low cost. This study aims to establish a rapid detection method for Salmonella based on LAMP technology. Methods: Primers were designed for Salmonella's specific conservative invA gene. Through primer screening and optimization of reaction conditions, and a LAMP method for detecting Salmonella with realtime fluorescence and visual observation results was established. The sensitivity and specificity of the method were assessed, and the accuracy was evaluated through the testing of Salmonella-contaminated and noncontaminated clinical samples. Results:The optimal reaction temperature of LAMP was 60-65 ℃, and the optimal reaction time was 25-30 minutes. The detection limits of real-time fluorescence and visual observation were both 1.4 pg/μL. There was no cross-reactivity observed with 22 non-Salmonella species, and the specificity was 100%.Additionally, 30 samples contaminated with Salmonella, 30 samples not contaminated with Salmonella, and 8 clinical samples identified as positive by bacterial culture and microbial mass spectrometry were tested. The positive coincidence rate of the detection system was 97.4% by real-time fluorescence and 89.5% by visual observation, the negative coincidence rate was 100%, and the total coincidence rate was 98.5% and 94.1%, respectively.Conclusions: In the scene of infection, primary hospital, disaster area treatment and other scenarios, the conditions of environment, equipment and personnel was limited, therefore, the established real-time fluorescence and visual lamp method can provide a powerful means for the rapid detection of Salmonella.

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Development of a duplex lateral flow dipstick test for the detection and differentiation of Listeria spp. and Listeria monocytogenes in meat products based on loop-mediated isothermal amplification

Cited by 22 publications

References 38 publications

State of the art: Lateral flow assays toward the point‐of‐care foodborne pathogenic bacteria detection in food samples

State of the art: Lateral flow assays toward the point‐of‐care foodborne pathogenic bacteria detection in food samples

Rapid Detection of Escherichia coli O157:H7 by Loop-Mediated Isothermal Amplification Coupled with a Lateral Flow Assay Targeting the z3276 Genetic Marker

Rapid detection of Salmonella based on loop-mediated isothermal amplification

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