Development of a Disposable Electrochemical Immunosensor for Detection of the Herbicide Acetochlor

Solná, Renáta; Skládal, Petr; Eremin, Sergei A.

doi:10.1080/0306731021000050877

Cited by 12 publications

(9 citation statements)

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“…A simplification on the assay procedure in terms of operator intervention can be achieved coupling immunosensor devices with flow systems [91,109,137,138,142,146,149,155]. Working with a flow system the procedure can be automated and the analysis performed continuously and quickly, but the systems become instrumentally more complex and none of the assay steps are removed.…”

Section: Immunosensor Formatsmentioning

confidence: 99%

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Recent Advances in Electrochemical Enzyme Immunoassays

2005

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Enzyme immunoassays (EIAs) are currently the predominant analytical technique for the quantitative determination of a broad variety of analytes in clinical, medical, biotechnological, and environmental significance. Although the most common detection methods for EIAs are based on spectroscopic measurements, electrochemical techniques, due to their high sensitivity, selectivity, simplicity and low cost, have emerged as a very attractive alternative to carry out the detection step in this kind of assays. The intention of this review is to cover the progress and development in integrating electrochemical detection methods with EIAs, over the past five years.

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Section: Immunosensor Formatsmentioning

confidence: 99%

“…The second strategy is most frequently used, but also requires the use of extreme pH solutions such as citric acid [146], NaOH À NaCl pH 12 [158,159] , citrate -phosphate pH 4.6 [110], phosphate pH 2.1 [109], pepsin pH 2.1 [155] or fluoride acid [160].…”

Section: Immunosensor Regenerationmentioning

confidence: 99%

Recent Advances in Electrochemical Enzyme Immunoassays

2005

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“…Earlier, we developed immunochemical methods for the determination of acetochlor by ELISA [22] and with the use of an immunosensor [23] or a piezosensor [24]. However, these are multistep procedures, and the time of analysis is 2 to 3 h. Moreover, ELISA includes a number of steps of pipetting and washing with the use of several specific reagents; it requires qualified personnel.…”

Section: Acetochlormentioning

confidence: 99%

Determination of the herbicide acetochlor by fluorescence polarization immunoassay

et al. 2005

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Acetochlor[2-chloro-N -(ethoxymethyl)-N -(2-ethyl-6-methylphenyl)acetamide] belongs to chloroacetanilide herbicides, which have been widely introduced into the world agricultural practice [1][2][3]. Chloroacetanilide herbicides are relatively unstable in the environment and undergo degradation within six months; this is a valuable property of these herbicides. However, they can be detected in reservoirs, fish, and products of plant origin in amounts higher than maximum permissible levels as a result of their agricultural use. Acetochlor is a comparatively new pesticide; it was first registered in the United States in 1994. It has now replaced related compounds because of its better biodegradability and a relatively small carcinogenic effect. However, as well as the majority of pesticides, acetochlor is genotoxic, as was exemplified in plants, rats, and human lymphocytes [1]. Therefore, acetochlor residues should be monitored in natural samples such as water and soil.Chromatographic techniques, such as gas chromatography with atomic emission [4] or mass-spectrometric detection [5, 6], high-performance liquid chromatography with various detection techniques [7,8], and capillary electrophoresis [9] are commonly used for determining herbicides, in particular, acetochlor. These techniques are highly sensitive and accurate; however, they are also expensive and time-consuming. At the same time, it is necessary to perform simple and rapid screening in a great number of samples in order to detect herbicide-contaminated areas.Immunochemical techniques based on the specific binding of an analyte to corresponding specific antibodies are well suitable for this purpose. Enzyme immunoassay (EIA) has received the widest acceptance; the theory and practice of EIA was described in detail [10]. The use of EIA for the determination of pesticides has been reported in many reviews [11][12][13][14][15][16].EIA techniques are highly sensitive and simple; they can be performed under field conditions at the sampling site [17]. Since the 1990s, EIA has been used with increasing frequency for the screening of pesticides in natural and food samples. The current status, applicability, and prospects for the widespread use of immunochemical techniques for the determination of pesticides in the environment were considered in a review [18]. Recent data on the development and use of EIA for pesticides in Russia were published in [19][20][21].Earlier, we developed immunochemical methods for the determination of acetochlor by ELISA [22] and with the use of an immunosensor [23] or a piezosensor [24]. However, these are multistep procedures, and the time of analysis is 2 to 3 h. Moreover, ELISA includes a number of steps of pipetting and washing with the use of several specific reagents; it requires qualified personnel. Therefore, the recent trend has been toward the development of nonseparation (homogeneous) immunoassay techniques, which significantly improve socalled high-throughput screening (HTS) [25]. Although an optimum immunoassay procedure for...

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“…Uma variedade de moléculas doadoras de elétrons pode reagir rapidamente com os compostos I e II, sendo que, para a enzima HRP pode-se utilizar como mediador de elétrons: hidroquinona [9,10], ácido ascórbico [11], ferroceno [12,13], iodeto [14,15,16], entre outros. O ácido 5-aminossalicílico (5-ASA) também tem sido usado como mediador para a enzima HRP em imunoensaios com detecção ótica [17], potenciométrica [18] e amperométrica [19,20,21], sendo que nos trabalhos utilizando a técnica amperométrica, a HRP foi imobilizada na superfície dos diferentes eletrodos.…”

Section: (5)unclassified

“…A molécula do mediador escolhido deve reagir rapidamente com a peroxidase oxidada. Uma variedade de moléculas doadoras de elétrons pode reagir rapidamente com os compostos I e II, sendo que, para a enzima HRP pode-se utilizar como mediador de elétrons: hidroquinona [9,10] [19,20,21], sendo que nos trabalhos utilizando a técnica amperométrica, a HRP foi imobilizada na superfície dos diferentes eletrodos.O presente trabalho apresenta um estudo sobre o comportamento eletroquímico da enzima HRP sobre eletrodo de grafite utilizando o peróxido de hidrogênio como substrato enzimático e o 5-ASA como mediador da transferência eletrônica. …”

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Estudo do comportamento eletroquímico da enzima peroxidase na presença de peróxido de hidrogênio e ácido 5-aminossalicílico

2008

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The electrochemical behavior of the enzyme peroxidase (HRP) was investigated using the hydrogen peroxide as enzymatic substrate and the 5-aminosalicylic acid (5-ASA) as mediator of electrons on graphite electrodes. Several parameters were optimized, namely, the applied potential to the amperometric technique fixed in -0.125V, the 0.1 mol L-1 phosphate-citrate buffer at pH 5.0 as supporting electrolyte and the proportion between the 5-ASA and H2O2 in 1:7, among others. It was observed the catalysis of the oxidation reaction of the H2O2 in the presence of the enzyme HRP and 5-ASA. The oxidation product was reduced in the electrode surface, evidencing a significant increase in the intensity of the cathodic current.

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Development of a Disposable Electrochemical Immunosensor for Detection of the Herbicide Acetochlor

Cited by 12 publications

References 0 publications

Recent Advances in Electrochemical Enzyme Immunoassays

Recent Advances in Electrochemical Enzyme Immunoassays

Determination of the herbicide acetochlor by fluorescence polarization immunoassay

Estudo do comportamento eletroquímico da enzima peroxidase na presença de peróxido de hidrogênio e ácido 5-aminossalicílico

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