Development of a cytochrome c-based screen-printed biosensor for the determination of the antioxidant capacity of orange juices

Cortina‐Puig, Montserrat; Muñoz-Berbel, Xavier; Rouillon, Régis; Calas‐Blanchard, Carole; Marty, Jean‐Louis

doi:10.1016/j.bioelechem.2009.04.004

Cited by 50 publications

(27 citation statements)

References 27 publications

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“…The biosensors were used for the determination of antioxidant capacity in wines, the results being consistent with those obtained by spectrophotometry [112,113] or in orange juices, by biosensors based on screen-printed electrodes [114]. For the analysis of commercial red wines, a multi-walled nanotube ionic liquid electrode with immobilized tyrosinase was used [113].…”

Section: Biosensors Methodssupporting

confidence: 64%

Methods for Total Antioxidant Activity Determination: A Review

Pisoschi¹,

Negulescu²

2012

Biochem & Anal Biochem

325

302

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Section: Biosensors Methodssupporting

confidence: 64%

Methods for Total Antioxidant Activity Determination: A Review

Pisoschi¹,

Negulescu²

2012

Biochem & Anal Biochem

325

302

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“…This difficulty has been overcome by using electrode modifiers such as metal oxides, advanced carbon materials, DNA or lipid membranes [20][21][22][23][24][25][26] . In particular, the modification of surfaces with organic thin films like self-assembled monolayers (SAM), has been extensively used to manipulate the electrode surface, promoting the appropriate orientation of the protein and thus enhancing its electroactivity [27][28][29][30][31][32][33][34][35][36] .…”

Section: Introductionmentioning

confidence: 99%

Direct Electron Transfer to Cytochrome c Induced by a Conducting Polymer

et al. 2017

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The direct electron transfer (DET) to redox proteins has become a central topic in the development of biotechnological devices. The present work explores the mechanisms of the direct electrochemistry between cytochrome c and a conducting polymer, PEDOT-PSS. This polymer has been electrosynthesized from its monomers in aqueous solution on gold electrodes and its capabilities for DET to cyt c have been examined by electrochemical and spectroelectrochemical methods. The polymer was electrodeposited with controlled thickness and we determined the electron transfer rate constant for cyt c oxidation was about 2 orders of magnitude higher than those obtained at conventional electrodes. Spectroelectrochemical measurements allowed to evaluate the redox state of the polymer as a function of the potential and, in addition, the observation of intrinsic cyt c redox activity upon electron transfer from the conducting polymer. During the oxidation process of this protein, lysine residues placed near the heme crevice interact electrostatically with the anionic polyelectrolyte PSS. This interaction favors the orientation of the heme group towards the chains of PEDOT backbone, which is the eventual responsible for the electron transfer to the protein.3

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“…Unauthenticated Download Date | 5/11/18 12:03 AM An amperometric biosensor employing two biorecognition elements, enabled the estimation of antioxidant activity of juices: the biosensor was developed by covalently co-immobilizing cyt c and xanthine oxidase on a mercaptoundecanol/mercaptoundecanoic acid self-assembled monolayer-modified screen-printed gold electrode. The viability of this technique was proved by estimating the antioxidant capacity of reference recognized antioxidants, such as ascorbic acid and Trolox as well as orange juices [132]. A further development of amperometric cytochrome c -based sensors involved cytochrome c and xanthine oxidase co-immobilization on the electrode by employing a composition-optimized self assembled monolayer of long-chain thiols.…”

Section: Amperometrymentioning

confidence: 99%

“…Though, even if characterized by a wide linear range, particularly in the case of nonenzymatic methods, the method suffers from the difficulty to electro-oxidize at constant potential high molecular mass antioxidants, and the measured signal does not always give account on their contribution [122]. Lowering the working potential is possible by changing the biocatalytical component or by using electrode modification with improved analytical characteristics in the case of high antioxidant contentsamples, such as juices, wines or teas [132,134,138].…”

Section: Critical and Comparative Considerations On The Electrochemicmentioning

confidence: 99%

Electrochemical Methods for Total Antioxidant Capacity and its Main Contributors Determination: A review

2015

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Abstract:Backround: The present review focuses on electrochemical methods for antioxidant capacity and its main contributors assessment. The main reactive oxygen species, responsible for low density lipoprotein oxidation, and their reactivity are reminded. The role of antioxidants in counteracting the factors leading to oxidative stress-related degenerative diseases occurence, is then discussed. Antioxidants can scavenge free radicals, can chelate pro-oxidative metal ions, or quench singlet oxygen. When endogenous factors (uric acid, bilirubin, albumin, metallothioneins, superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione-

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Development of a cytochrome c-based screen-printed biosensor for the determination of the antioxidant capacity of orange juices

Cited by 50 publications

References 27 publications

Methods for Total Antioxidant Activity Determination: A Review

Methods for Total Antioxidant Activity Determination: A Review

Direct Electron Transfer to Cytochrome c Induced by a Conducting Polymer

Electrochemical Methods for Total Antioxidant Capacity and its Main Contributors Determination: A review

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