Development of a cryopreservation protocol for long-term storage of black tiger shrimp (Penaeus monodon) spermatophores

Assessment of viability of sperm cells of Litopenaeus vannamei on cryopreservation

“…Of a total of 74 spermatophores, two were obtained by cryopreservation of the sperm suspension without any cryoprotectant agent and used as control. The remaining 72 spermatophores were divided in the treatments with two distinct cryoprotectants after a selection based on cryoprotectant toxicity tests carried out for penaeidae by diverse authors (Gwo, 2000;Alfaro et al 2001;Lezcano et al 2004;Vuthiphandchai et al 2007). One group of spermatophores was submitted to DMSO and the other to EG, both at 10% concentration and an equilibration time of 10 minutes, based on their prior toxicity tests.…”

“…Semen cryopreservation of fish is already possible and many manipulation techniques are applied in some species, with special highlights for the salmonidae (Scott and Baynes 1980), siluridae (Legendre et al 1996), cyprinids (Billard et al 2004), and characidae (Carosfeld et al 2003). However, there are only a few studies on crustaceans and penaeidae concerning this matter (Lezcano et al 2004, Vuthiphandchai et al 2007Salazar et al 2008). The development of methods of semen preservation and the implementation of effective artificial reproduction protocols require a good knowledge of the reproductive biology and seminal characteristics of the targeted species (Ninhaus-Silveira et al 2006).…”

“…The development of methods of semen preservation and the implementation of effective artificial reproduction protocols require a good knowledge of the reproductive biology and seminal characteristics of the targeted species (Ninhaus-Silveira et al 2006). For marine shrimps, cryoprotectants dimethyl sulfoxide (DMSO) and ethylene glycol (EG) are widely used (Anchordoguy et al 1988;Barth et al 2006;Vuthiphandchai et al 2007). DMSO, when compared with glycerol, proline and trehalose, presents low toxicity to the spermatic cells of Sicyonia ingentis (Anchordoguy et al 1988).…”

“…DMSO, when compared with glycerol, proline and trehalose, presents low toxicity to the spermatic cells of Sicyonia ingentis (Anchordoguy et al 1988). For Penaeus monodon, Vuthiphandchai et al (2007) reported that DMSO, etylenoglicol and sucrose had low toxicity to embryos, with survival ranging of 68.0 to 82.3%. DMSO, etylenoglicol and methanol were used to cryopreserve the spermatophores of the same specie for 48h, and the higher survival (79.7 ± 0.4%) was observed with DMSO (5%) (Bart et al 2006).…”

Induction of acrosome reaction of spermatozoa in Eriocheir sinensis by low temperature

“…Temperature, which is one of the most important factors that affect the physiology of cell (Boon et al 2008;Fan et al 2008;Jong et al 2009;Oguchi et al 2006), is one of them (Martin et al 2001;Nagao and Munehara 2007). Low temperature has been widely used for in vitro spermatozoa storage and artificial fertilization of aquatic crustaceans (Bart et al 2006;Magda et al 2004;Nimrat et al 2006;Thomas et al 1988;Vuthiphandchai et al 2007). However, the information on the effects of temperature on AR and capacitation in crabs are sparse (Bhavanishankar and Subramoniam1997;Kang et al 2009) although aquaculture of crabs has become very important.…”