Development of a conductive biocomposite combining graphene and amniotic membrane for replacement of the neuronal network of tissue-engineered urinary bladder

Adamowicz, Jan; Pasternak, Iwona; Kloskowski, Tomasz; Gniadek, M.; Breda, S. Â. V. Van; Bühl, Monika; Balcerczyk, Daria; Gagat, Maciej; Grzanka, Dariusz; Strupiński, Włodek; Pokrywczyńska, Marta

doi:10.1038/s41598-020-62197-3

Cited by 21 publications

(12 citation statements)

References 45 publications

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“…The high reinforcing properties of graphene are due to the two-dimensional structure with high specific surface area of 2630 m 2 g −1 26 . Graphene, having good biological properties, has been extensively studied in biological applications, including drug delivery 27 , biosensing 28 , tissue engineering 29 , and bioimaging 30 .…”

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confidence: 99%

Improving the mechanical behavior of reduced graphene oxide/hydroxyapatite nanocomposites using gas injection into powders synthesis autoclave

Nosrati

Sarraf-Mamoory

et al. 2020

Sci Rep

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in this study, we show the synthesis of reduced graphene oxide/hydroxyapatite (rGo/HA) composites using a hydrothermal autoclave with argon-15% hydrogen gas injection. This both increases the hydrothermal pressure and uses hydrogen as a reductive agent in the process. The synthesized powders were then consolidated with spark plasma sintering method. The analysis of the consolidated samples included Vickers Indentation technique and cell viability. The results showed that injected gases in the autoclave produced powders with a higher crystallinity compared to synthesis without the gases. Also, hydrogen gas led to increased reduction of GO. The microscopic analysis confirmed existing graphene sheets with folding and wrinkling in the powders and indicated that various preferential directions played a role in the growth of hydroxyapatite crystals. The results showed that in general, graphene sheets increased the mechanical properties of HA. In the samples synthesized with injected gases, this increase was more significant. Interface analysis results indicate that reduced graphene oxide (rGO)/HA interface is likely coherent. These nanocomposites were biocompatible and showed some hydrophobicity compared to pure HA.

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confidence: 99%

Improving the mechanical behavior of reduced graphene oxide/hydroxyapatite nanocomposites using gas injection into powders synthesis autoclave

Nosrati

Sarraf-Mamoory

et al. 2020

Sci Rep

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“…GO is a single-atom layered material composed of carbon, hydrogen and oxygen molecules through the oxidation of inexpensive and abundant graphite crystals [ 30 , 44 , 45 ]. Furthermore, GO has good biological properties and has been widely used in biomedical field, including drug delivery [ 46 ], cancer therapy [ 47 , 48 ], biosensing [ 49 ], tissue engineering [ 50 ], and bioimaging [ 51 ]. The use of GO can enhance cell proliferation and differentiation characteristics.…”

Section: Discussionmentioning

confidence: 99%

Electrophoresis-Aided Biomimetic Mineralization System Using Graphene Oxide for Regeneration of Hydroxyapatite on Dentin

et al. 2021

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Graphene oxide (GO) is an emerging luminescent carbon nanomaterial with the ability to foster hydroxyapatite (HA). A specially designed electrophoresis system can be used to accelerate the mineralization process. The aim of this study was to promote HA crystal growth on demineralized dentin using a GO incorporated electrophoresis system. GO was successfully synthesized by carbonization of citric acid and its presence was confirmed by Fourier transform infrared and UV-visible spectrophotometry evaluation. Dentin slices were placed in demineralized solution and divided into control (without the electrophoresis system) and experimental group. Demineralized dentin slices in the experimental group were remineralized using the electrophoresis system for 8 h/1.0 mA, with one subgroup treated without GO and the other with GO. Energy dispersive spectroscopy evaluation showed that the calcium/phosphate ratio of the crystal formed in control and experimental group with addition of GO was close to natural hydroxyapatite. However, scanning electron microscopy evaluation showed that the exposed dentinal tubules were occluded with rod-like crystals, which is similar to native enamel morphology, in the experimental group with addition of GO compared to the flake-like crystal in the control group. Mechanical evaluation revealed that the nanohardness and modulus of remineralized dentin were significantly higher in the experimental group. In conclusion, GO is a promising material to remineralize dentin and the introduction of an electrophoresis system can accelerate its process.

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“…The constructs have been seeded with porcine UCs and SMCs derived from porcine detrusor. It has been demonstrated that the presence of graphene layer significantly increased electrical conductivity of the bio-composite and the contractile response indicated the effective SMCs stimulation [68].…”

Section: Mechanical Stimulation Of Scaffold Using a Bioreactormentioning

confidence: 99%

Bladder Substitution: The Role of Tissue Engineering and Biomaterials

2021

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Tissue engineering could play a major role in the setting of urinary diversion. Several conditions cause the functional or anatomic loss of urinary bladder, requiring reconstructive procedures on the urinary tract. Three main approaches are possible: (i) incontinent cutaneous diversion, such as ureterocutaneostomy, colonic or ileal conduit, (ii) continent pouch created using different segments of the gastrointestinal system and a cutaneous stoma, and (iii) orthotopic urinary diversion with an intestinal segment with spherical configuration and anastomosis to the urethra (neobladder, orthotopic bladder substitution). However, urinary diversions are associated with numerous complications, such as mucus production, electrolyte imbalances and increased malignant transformation potential. In this context, tissue engineering would have the fundamental role of creating a suitable material for urinary diversion, avoiding the use of bowel segments, and reducing complications. Materials used for the purpose of urinary substitution are biological in case of acellular tissue matrices and naturally derived materials, or artificial in case of synthetic polymers. However, only limited success has been achieved so far. The aim of this review is to present the ideal properties of a urinary tissue engineered scaffold and to examine the results achieved so far. The most promising studies have been highlighted in order to guide the choice of scaffolds and cells type for further evolutions.

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Development of a conductive biocomposite combining graphene and amniotic membrane for replacement of the neuronal network of tissue-engineered urinary bladder

Cited by 21 publications

References 45 publications

Improving the mechanical behavior of reduced graphene oxide/hydroxyapatite nanocomposites using gas injection into powders synthesis autoclave

Improving the mechanical behavior of reduced graphene oxide/hydroxyapatite nanocomposites using gas injection into powders synthesis autoclave

Electrophoresis-Aided Biomimetic Mineralization System Using Graphene Oxide for Regeneration of Hydroxyapatite on Dentin

Bladder Substitution: The Role of Tissue Engineering and Biomaterials

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