Development of a colorimetric nucleic acid-based lateral flow assay with non-biotinylated capture DNA

Javani, Atefeh; Javadi‐Zarnaghi, Fatemeh; Rasaee, Mohammad Javad

doi:10.1007/s13765-017-0321-9

Cited by 17 publications

(10 citation statements)

References 43 publications

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“…Nowadays, the development of nucleic-acid-based lateral flow assays (NALFAs) is attracting increasing interest. NALFAs are widely used for the detection of several targets, such as DNA sequences, biomarkers, metal ions, and drugs of abuse [ 22 , 23 , 24 , 25 ]. Most of the reported NALFAs are designed with aptamers or single-strand DNAs as sensing probes, and they are commonly used for hybridization-based platforms, where the detection is performed by the complementary recognition of two oligonucleotide strands.…”

Section: Resultsmentioning

confidence: 99%

A Lateral Flow Device for Point-of-Care Detection of Doxorubicin

2022

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A simple, rapid, and sensitive point-of-care (POC) device for the on-site detection of doxorubicin was developed. The proposed method relies on the naked-eye detection of the intrinsic fluorescence of the drug in a lateral flow device (LFD) configuration, exploiting the biological recognition of DNA probes and avoiding the use of expensive antibodies and sophisticated instrumentations. The POC assay does not require any pre-treatment or purification step and provides an immediate visual readout, achieving a limit of detection as low as ca. 1 ng doxorubicin, outperforming several laboratory-based instrumental techniques. The POC method was proven useful for the detection of trace amounts of the drug both in the case of water solutions (to simulate surface contaminations) and in urine samples, opening promising perspectives for routine monitoring of doxorubicin, with potential benefit to healthcare workers and personalized chemotherapies.

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Section: Resultsmentioning

confidence: 99%

A Lateral Flow Device for Point-of-Care Detection of Doxorubicin

2022

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“…The composition of the running solution can strongly affect LFA performances, partly because undesirable interfacial problems can occur. In particular, nonspecific interactions with capture or detection antibodies or steric hindrance on the NP or nitrocellulose membrane can modify the background signal on the membrane as well as the assay sensitivity. , Based on empirical observations, it is generally accepted that a relatively high salt concentration increases the reproducibility and sensitivity of the LFA by controlling the pH and ionic strength of the running solution, thus avoiding protein denaturation. , On the other hand, zwitterionic or nonionic detergents, such as CHAPS or Tween 20, (i) stabilize proteins (particularly membrane proteins), (ii) can renature antibody epitopes, (iii) allow the sample to flow through the strip, (iv) elute antigens adsorbed nonspecifically on the membrane, and (v) act as blocking agents to saturate free binding sites, alone or in combination with proteins (BSA, ovalbumin, casein, etc), thereby reducing background on the membrane. − The addition of sodium azide also protects buffers from microbial contamination …”

Section: Methodsmentioning

confidence: 99%

Multititration: The New Method for Implementing Ultrasensitive and Quantitative Multiplexed In-Field Immunoassays Despite Cross-Reactivity?

Mousseau,

Féraudet Tarisse,

Simon

et al. 2023

Anal. Chem.

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“…The nucleic-acid-based LFAs offer a unique platform for detecting specific DNA or RNA sequences, which is difficult to be performed with immunoassay-based LFAs. [39] There are two strategies in LFAs commonly used for detecting nucleic acid targets: direct DNA sequence detection and nucleic acid lateral flow immunoassay (NALFIA). [40,41] The nucleic acid direct detection method in LFA involves the immobilization of nucleic acid probes in the test and or control zone, which will capture the nucleic acid targets.…”

Section: Lfasmentioning

confidence: 99%

Point‐of‐Care Testing (POCT) Devices for DNA Detection: A Comprehensive Review

Mahardika,

Naorungroj,

Khamcharoen

et al. 2023

Advanced NanoBiomed Research

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DNA chips play a crucial role in point‐of‐care diagnostics by enabling rapid and accurate detection of genetic information. These chips offer high sensitivity and selectivity, allowing for the identification of specific DNA sequences associated with diseases and pathogens. Integration into lab‐on‐chip platforms streamlines and miniaturizes diagnostic workflows, paving the way for cost‐effective, portable, and user‐friendly testing devices that can revolutionize healthcare delivery. In this review, a comprehensive description of the platforms utilized in DNA analysis, including microfluidic devices and integrated DNA chips, is provided. It explores the selection and immobilization of DNA probes for improved selectivity. Additionally, it covers diverse detection techniques such as optical detection (colorimetry and fluorescence) and electrochemical techniques. In these discussions, it is aimed to provide a thorough understanding of the current state of the art in DNA biosensor‐integrated lab‐on‐chip technology for point‐of‐care testing. The continued advancements in DNA chip technology hold immense promise for the development of next‐generation point‐of‐care diagnostics, where integrated sample preparation and rapid results generation can further enhance patient outcomes and contribute to the effective management of diseases.

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Development of a colorimetric nucleic acid-based lateral flow assay with non-biotinylated capture DNA

Cited by 17 publications

References 43 publications

A Lateral Flow Device for Point-of-Care Detection of Doxorubicin

A Lateral Flow Device for Point-of-Care Detection of Doxorubicin

Multititration: The New Method for Implementing Ultrasensitive and Quantitative Multiplexed In-Field Immunoassays Despite Cross-Reactivity?

Point‐of‐Care Testing (POCT) Devices for DNA Detection: A Comprehensive Review

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